Methanol-based biomanufacturing of fuels and chemicals using native and synthetic methylotrophs
Synthetic and Systems Biotechnology, 2023 Methanol has recently gained significant attention as a potential carbon substrate for the production of fuels and chemicals, owing to its high degree of reduction, abundance, and low price.
Arslan Sarwar, Eun Yeol Lee
doaj +1 more source
Production, purification and characterization of a milk clotting enzyme from Bacillus methanolicus LB-1. [PDF]
Food Sci Biotechnol, 2019 Bacillus methanolicus LB-1 isolated from traditional rice wine was found to produce a milk clotting enzyme (MCE), and its fermentation conditions were optimized using response surface methodology. Then the MCE was produced by ethanol precipitation, and further chromatography separation resulted in a 10.46-fold purification with a 59.28% recovery.
Li L +5 more
europepmc +4 more sources
Metabolic Engineering of Bacillus methanolicus MGA3 for Production of Acetoin [PDF]
, 2021 Being a pure, inexpensive, non-food chemical that can be sustainably produced, methanol has attracted great interest as a carbon feedstock for microbial production of commodity/specialty chemicals. In this thesis, methanol-based production of acetoin was
Chan, Dennis Tin Chat
core
Transcriptome analysis of thermophilic methylotrophic Bacillus methanolicus MGA3 using RNA-sequencing provides detailed insights into its previously uncharted transcriptional landscape. [PDF]
BMC Genomics, 2015 Background Bacillus methanolicus MGA3 is a thermophilic, facultative ribulose monophosphate (RuMP) cycle methylotroph. Together with its ability to produce high yields of amino acids, the relevance of this microorganism as a promising candidate for ...
Irla M +5 more
europepmc +4 more sources
Functional characterization of key enzymes involved in L-glutamate synthesis and degradation in the thermotolerant and methylotrophic bacterium Bacillus methanolicus. [PDF]
Appl Environ Microbiol, 2013 Krog A +3 more
europepmc +3 more sources
Characterization of two transketolases encoded on the chromosome and the plasmid pBM19 of the facultative ribulose monophosphate cycle methylotroph Bacillus methanolicus. [PDF]
BMC Microbiol, 2014 Markert B +3 more
europepmc +3 more sources
Establishing 3-hydroxypropionic acid production in Bacillus methanolicus MGA3 by heterologous expression of malonyl coenzyme A reductase and malonic semialdehyde reductase [PDF]
, 2022 3-Hydroxypropionsyre regnes å være en av de mest sentrale biobaserte kjemikaliene som kan produseres fra fornybare karbohydrater. Syren brukes som en forløper for flere ulike kjemiske forbindelser i produksjon av forbruksvarer.
Jacobsen, Robert Torsvik
core +1 more source
ChemBioChem, Volume 21, Issue 22, Page 3273-3281, November 16, 2020., 2020 Propelled to propene: Propene is produced in a three‐step enzymatic cascade from n‐butanol via butanal and butyric acid, with the last step being oxidative decarboxylation catalyzed by OleTJE. Variation of the supply of redox donors demonstrates the peroxygenase nature of OleTJE.
Daniel Bauer +2 more
wiley +1 more source
Biotechnology and Bioengineering, Volume 117, Issue 11, Page 3422-3434, November 2020., 2020 Wenk, Schann and coworkers have constructed an Escherichia coli strain deleted in dihydrolipoyl dehydrogenase, which participates in the pyruvate and 2‐ketoglutarate dehydrogenase complexes. When cultivated on acetate, this strain is auxotrophic to NADH and ATP, and thus provides a useful platform for comparing and optimizing enzymatic systems for ...
Sebastian Wenk +6 more
wiley +1 more source
Syngas and Methanol‐Based Biorefinery Concepts
Chemie Ingenieur Technik, Volume 92, Issue 11, Page 1680-1699, November 2020., 2020 The use of syngas, which can be derived from CO2 or exhaust gases of steel mills, is gaining interest for the synthesis of added‐value products. In this review, the state of the art and technological challenges emerging from the use of this gaseous feedstock are described, as well as the possibility of converting syngas into methanol and its subsequent
Cláudio J. R. Frazão, Thomas Walther
wiley +1 more source