Results 41 to 50 of about 115 (93)

Cloning, expression, and sequence analysis of the Bacillus methanolicus C1 methanol dehydrogenase gene [PDF]

Journal of Bacteriology, 1992
The gene (mdh) coding for methanol dehydrogenase (MDH) of thermotolerant, methylotroph Bacillus methanolicus C1 has been cloned and sequenced. The deduced amino acid sequence of the mdh gene exhibited similarity to those of five other alcohol dehydrogenase (type III) enzymes, which are distinct from the long-chain zinc-containing (type I) or short ...
de Vries, Gerhard, Arfman, Nico, Terpstra, Peter, Dijkhuizen, Lubbert   +3 more
openaire   +3 more sources

Genetic Tools and Techniques for Recombinant Expression in Thermophilic Bacillaceae

Microorganisms, 2018
Although Escherichia coli and Bacillus subtilis are the most prominent bacterial hosts for recombinant protein production by far, additional species are being explored as alternatives for production of difficult-to-express proteins.
Eivind B. Drejer, Sigrid Hakvåg, Marta Irla, Trygve Brautaset   +3 more
doaj   +1 more source

Engineering Methylotrophic Yeasts as Cell Factories for Chemical Production Using Methanol as a Feedstock

GCB Bioenergy, Volume 17, Issue 8, August 2025.
Methanol, a sustainable and abundant one‐carbon feedstock, can be utilized by natural methylotrophic yeasts to synthesize valuable chemicals. However, methanol‐based manufacturing with these yeasts faces several challenges, including low assimilation efficiency, carbon loss, and methanol toxicity. This review summarizes and discusses recent engineering
Shuxian Wang, Jiayu Fang, Yanping Zhang, Yin Li, Taicheng Zhu   +4 more
wiley   +1 more source

High‐quality genome of a novel Thermosynechococcaceae species from Namibia and characterization of its protein expression patterns at elevated temperatures

MicrobiologyOpen, Volume 13, Issue 5, October 2024.
In this study, the genome of a new thermophilic cyanobacterium, Thermosynechococcaceae cyanobacterium sp. Okahandja, isolated from a hot spring near Okahandja in Namibia, was sequenced. Additionally, cultivations were conducted at elevated temperatures of 40, 50, and 55°C, followed by analyses of the respective adapted proteomes based on the annotated ...
Nathanael D. Arnold, Michael Paper, Tobias Fuchs, Nadim Ahmad, Patrick Jung, Michael Lakatos, Katia Rodewald, Bernhard Rieger, Farah Qoura, Martha Kandawa‐Schulz, Norbert Mehlmer, Thomas B. Brück   +11 more
wiley   +1 more source

Harnessing the thermotolerant methylotroph Bacillus methanolicus for methanol-based synthetic L-proline production

Microbial Cell Factories
Background Bacillus methanolicus MGA3 is a thermotolerant methylotroph that utilizes methanol, a renewable C₁ substrate, as its sole carbon and energy source.
Christine Frank, David Virant, Gregor Kosec, Tamara Hoffmann, Oskar Zelder, Max F. Felle, Erhard Bremer   +6 more
doaj   +1 more source

Design, construction and optimization of formaldehyde growth biosensors with broad application in biotechnology

Microbial Biotechnology, Volume 17, Issue 7, July 2024.
In this study, we engineered Escherichia coli to function as cellular biosensors capable of detecting a broad range of formaldehyde concentrations. By using natural and promiscuous formaldehyde assimilation enzymes, we created three distinct growth biosensor strains that rely on formaldehyde for cell growth, demonstrating sensitivity from approximately
Karin Schann, Jenny Bakker, Maximilian Boinot, Pauline Kuschel, Hai He, Maren Nattermann, Nicole Paczia, Tobias Erb, Arren Bar‐Even, Sebastian Wenk   +9 more
wiley   +1 more source

Process Cascade for the Production of Green Polymers from CO2 and Electric Energy

Chemie Ingenieur Technik, Volume 96, Issue 5, Page 698-712, May 2024.
A novel process cascade is introduced that combines the advantages of electrochemical CO2 conversion as CCU approach with the unrivaled synthetic potential of industrial biotechnology: CO2 is electrocatalytically reduced to formic acid which serves as substrate for the metabolically engineered bacterium Methylorubrum extorquens that prduces l‐lysine as
Jonathan Thomas Fabarius, Carsten Pietzka, Dhananjai Pangotra, Benjamin Wriedt, Luciana Vieira, Carina Sagstetter, Melanie Speck, Athanassios Ziogas, Nils Baumgarten, Hans‐Joachim Kost, Patrick Löb, Katja Patzsch, Catherine Bernau, Sarah Boeringer, Davide Pico, Antje Lieske, Marcus Vater, Ulrich Wendler, Arne Roth   +18 more
wiley   +1 more source

Constructing efficient bacterial cell factories to enable one‐carbon utilization based on quantitative biology: A review

Quantitative Biology, Volume 12, Issue 1, Page 1-14, March 2024.
Abstract Developing methylotrophic cell factories that can efficiently catalyze organic one‐carbon (C1) feedstocks derived from electrocatalytic reduction of carbon dioxide into bio‐based chemicals and biofuels is of strategic significance for building a carbon‐neutral, sustainable economic and industrial system.
Yazhen Song, Chenxi Feng, Difei Zhou, Zengxin Ma, Lian He, Cong Zhang, Guihong Yu, Yan Zhao, Song Yang, Xinhui Xing   +9 more
wiley   +1 more source

Structural and catalytic properties of the peroxygenase P450 enzyme CYP152K6 from Bacillus methanolicus

Journal of Inorganic Biochemistry, 2018
The CYP152 family of cytochrome P450 enzymes (P450s or CYPs) are bacterial peroxygenases that use hydrogen peroxide to drive hydroxylation and decarboxylation of fatty acid substrates. We have expressed and purified a novel CYP152 family member - CYP152K6 from the methylotroph Bacillus methanolicus MGA3.
Girvan, Hazel, Poddar, Harshwardhan, Mclean, Kirsty, Nelson, David R., Hollywood, Katherine; id_orcid 0000-0002-7028-047X, Levy, Colin; id_orcid 0000-0002-9724-310X, Leys, David, Munro, Andrew   +7 more
openaire   +3 more sources

Identification of overoxidizing and non-overoxidizing NAD-dependent methanol dehydrogenases and implications for synthetic methylotrophy

Nature Communications
Synthetic methylotrophy offers opportunities for sustainable chemical and biofuel production. While recently established methylotrophic E. coli can grow on methanol, undesirable formate accumulation occurs during growth and bioproduction.
Philipp Keller, Emese Hegedis, Benedikt Jäger, Simon H. Rüdisser, Hedwig Schultz, Lars A. Büchel, Andrea M. Ochsner, Timothy Bradley, Michael A. Reiter, Donald Hilvert, Julia A. Vorholt   +10 more
doaj   +1 more source