Results 31 to 40 of about 115 (93)

Methanol-based biomanufacturing of fuels and chemicals using native and synthetic methylotrophs

Synthetic and Systems Biotechnology, 2023
Methanol has recently gained significant attention as a potential carbon substrate for the production of fuels and chemicals, owing to its high degree of reduction, abundance, and low price.
Arslan Sarwar, Eun Yeol Lee
doaj   +1 more source

Engineering Escherichia coli for Poly-β-hydroxybutyrate Production from Methanol

Bioengineering, 2023
The naturally occurring one-carbon assimilation pathways for the production of acetyl-CoA and its derivatives often have low product yields because of carbon loss as CO2.
Jiaying Wang, Zhiqiang Chen, Xiaogui Deng, Qianqian Yuan, Hongwu Ma   +4 more
doaj   +1 more source

Interrogating the Role of the Two Distinct Fructose-Bisphosphate Aldolases of Bacillus methanolicus by Site-Directed Mutagenesis of Key Amino Acids and Gene Repression by CRISPR Interference

Frontiers in Microbiology, 2021
The Gram-positive Bacillus methanolicus shows plasmid-dependent methylotrophy. This facultative ribulose monophosphate (RuMP) cycle methylotroph possesses two fructose bisphosphate aldolases (FBA) with distinct kinetic properties.
Kerstin Schultenkämper, Desirée D. Gütle, Marina Gil López, Laura B. Keller, Lin Zhang, Oliver Einsle, Jean-Pierre Jacquot, Volker F. Wendisch   +7 more
doaj   +1 more source

Production of Propene from n‐Butanol: A Three‐Step Cascade Utilizing the Cytochrome P450 Fatty Acid Decarboxylase OleTJE

ChemBioChem, Volume 21, Issue 22, Page 3273-3281, November 16, 2020., 2020
Propelled to propene: Propene is produced in a three‐step enzymatic cascade from n‐butanol via butanal and butyric acid, with the last step being oxidative decarboxylation catalyzed by OleTJE. Variation of the supply of redox donors demonstrates the peroxygenase nature of OleTJE.
Daniel Bauer, Ioannis Zachos, Volker Sieber   +2 more
wiley   +1 more source

Development of a Novel Assay for Synthesis and Hydrolysis of Sedoheptulose 1,7-bisphosphate (SBP) in vitro by Combinations of Purified Fructose 1,6-bisphosphate aldolases (FBA) Proteins and Fructose 1,6-bisphosphatases (FBPase) Proteins from Bacillus methanolicus MGA3

Bio-Protocol, 2014
Bacillus methanolicus (B. methanolicus) is a Gram-positive, thermotolerant, and facultative methylotrophic bacterium that can use the one-carbon (C1) compound methanol as a source of carbon and energy (Schendel et al., 1990; Arfman et al., 1997; Arfman ...
Jessica Stolzenberger, Steffen Lindner, Marcus Persicke, Trygve Brautaset, Volker Wendisch   +4 more
doaj   +1 more source

An “energy‐auxotroph” Escherichia coli provides an in vivo platform for assessing NADH regeneration systems

Biotechnology and Bioengineering, Volume 117, Issue 11, Page 3422-3434, November 2020., 2020
Wenk, Schann and coworkers have constructed an Escherichia coli strain deleted in dihydrolipoyl dehydrogenase, which participates in the pyruvate and 2‐ketoglutarate dehydrogenase complexes. When cultivated on acetate, this strain is auxotrophic to NADH and ATP, and thus provides a useful platform for comparing and optimizing enzymatic systems for ...
Sebastian Wenk, Karin Schann, Hai He, Vittorio Rainaldi, Seohyoung Kim, Steffen N. Lindner, Arren Bar‐Even   +6 more
wiley   +1 more source

Syngas and Methanol‐Based Biorefinery Concepts

Chemie Ingenieur Technik, Volume 92, Issue 11, Page 1680-1699, November 2020., 2020
The use of syngas, which can be derived from CO2 or exhaust gases of steel mills, is gaining interest for the synthesis of added‐value products. In this review, the state of the art and technological challenges emerging from the use of this gaseous feedstock are described, as well as the possibility of converting syngas into methanol and its subsequent
Cláudio J. R. Frazão, Thomas Walther
wiley   +1 more source

Microbial Engineering for Production of N‐Functionalized Amino Acids and Amines

Biotechnology Journal, Volume 15, Issue 7, July 2020., 2020
Microbial production of N‐functionalized amines and amino acids is reviewed in terms of recent advances and applications and the prospects of developing new fermentation processes. N‐functionalizing enzymes and strain engineering are focused here. Abstract N‐functionalized amines play important roles in nature and occur, for example, in the antibiotic ...
Melanie Mindt, Tatjana Walter, Pierre Kugler, Volker F. Wendisch   +3 more
wiley   +1 more source

Impact of CRISPR interference on strain development in biotechnology

Biotechnology and Applied Biochemistry, Volume 67, Issue 1, Page 7-21, January/February 2020., 2020
CRISPR interference drives metabolic engineering in biotechnology. Abstract Genetic perturbation systems are of great interest to redirect metabolic fluxes for value‐added production, as well as genetic screening for the development of new drugs, or to identify new targets for biotechnological applications. Here, we review CRISPR interference (CRISPRi),
Kerstin Schultenkämper, Luciana F. Brito, Volker F. Wendisch   +2 more
wiley   +1 more source

Genomics of Methylotrophy in Gram-Positive Methylamine-Utilizing Bacteria

Microorganisms, 2015
Gram-positive methylotrophic bacteria have been known for a long period of time, some serving as model organisms for characterizing the specific details of methylotrophy pathways/enzymes within this group. However, genome-based knowledge of methylotrophy
Tami L. McTaggart, David A. C. Beck, Usanisa Setboonsarng, Nicole Shapiro, Tanja Woyke, Mary E. Lidstrom, Marina G. Kalyuzhnaya, Ludmila Chistoserdova   +7 more
doaj   +1 more source