Engineering Escherichia coli for Poly-β-hydroxybutyrate Production from Methanol [PDF]
Bioengineering, 2023 The naturally occurring one-carbon assimilation pathways for the production of acetyl-CoA and its derivatives often have low product yields because of carbon loss as CO2.
Jiaying Wang +4 more
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Development of a Novel Assay for Synthesis and Hydrolysis of Sedoheptulose 1,7-bisphosphate (SBP) in vitro by Combinations of Purified Fructose 1,6-bisphosphate aldolases (FBA) Proteins and Fructose 1,6-bisphosphatases (FBPase) Proteins from Bacillus methanolicus MGA3 [PDF]
Bio-Protocol, 2014 Bacillus methanolicus (B. methanolicus) is a Gram-positive, thermotolerant, and facultative methylotrophic bacterium that can use the one-carbon (C1) compound methanol as a source of carbon and energy (Schendel et al., 1990; Arfman et al., 1997; Arfman ...
Jessica Stolzenberger +4 more
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Genetiske verktøy i Bacillus methanolicus
, 2011 Produksjon av aminosyrer ved hjelp av industriell bioteknologi er et voksende marked. I dag finnes det ingen kjente metylotrofe bakterier som benyttes til industriell produksjon av aminosyrer, men en attraktiv kandidat for framtidig produksjon er den ...
Andersen, Elisabeth Margrete Stien
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Expression of Recombinant Green Fluorescent Protein in Bacillus methanolicus
, 2011 Microbial biocatalysts are used in a wide range of industries to produce large scale quantities of proteins, amino acids, and commodity chemicals. Most of these processes use glucose or other low-cost sugars as the substrate.
Nilasari, Dewi
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Cell ReportsSummary: Bacillus methanolicus, a unique plasmid-dependent and thermophilic methylotroph, is an ideal chassis for one-carbon (C1) biomanufacturing. Despite its evolutionary uniqueness and industrial promise, the synthetic biology toolkit remains limited ...
Pan Liu +14 more
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Identification of overoxidizing and non-overoxidizing NAD-dependent methanol dehydrogenases and implications for synthetic methylotrophy [PDF]
Nature CommunicationsSynthetic methylotrophy offers opportunities for sustainable chemical and biofuel production. While recently established methylotrophic E. coli can grow on methanol, undesirable formate accumulation occurs during growth and bioproduction.
Philipp Keller +10 more
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Design, construction and optimization of formaldehyde growth biosensors with broad application in biotechnology. [PDF]
Microb BiotechnolIn this study, we engineered Escherichia coli to function as cellular biosensors capable of detecting a broad range of formaldehyde concentrations. By using natural and promiscuous formaldehyde assimilation enzymes, we created three distinct growth biosensor strains that rely on formaldehyde for cell growth, demonstrating sensitivity from approximately
Schann K +9 more
europepmc +2 more sources
Catabolic diversity of the facultative methylotroph Bacillus methanolicus
, 2020 Gil Lopez M. Catabolic diversity of the facultative methylotroph Bacillus methanolicus.
Gil Lopez, Marina
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Journal of Biotechnology, 2014 Irla M, Neshat A, Winkler A, et al. Complete genome sequence of Bacillus methanolicus MGA3, a thermotolerant amino acid producing methylotroph. Journal of Biotechnology.
Wendisch, Volker F. ; https://orcid.org/ +8 more
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Studie av forskjellige promotorer i den termotolerante metylotrofe bakterien Bacillus methanolicus
, 2019 Bacillus methanolicus er en termotolerant, metylotrof, Gram-positiv bakterie som har vist seg å gi høyt utbytte av aminosyrene glutamat og lysin ved overuttrykkelse av gener. Metylotrofi og toleranse mot høy dyrkningstemperatur gjør Bacillus methanolicus
Jelstad, Eirik
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