Results 11 to 20 of about 664 (136)

CRISPR-Cas9-driven genome editing in Bacillus methanolicus MGA3 [PDF]

Frontiers in Microbiology
Bacillus methanolicus is a thermophilic methylotroph that uses methanol as a sustainable feedstock in biotechnological industry. Here, we developed the CRISPR-Cas9 genome editing tool for B. methanolicus MGA3.
May L. K. Khider, Marta Irla, Marina Gil López, Anna Gispert, Thomas Konjetzko, Meliawati Meliawati, Jochen Schmid, Jochen Schmid, Trygve Brautaset, Luciana Fernandes Brito   +9 more
doaj   +8 more sources

Production of Value-Added Chemicals by Bacillus methanolicus Strains Cultivated on Mannitol and Extracts of Seaweed Saccharina latissima at 50°C [PDF]

Frontiers in Microbiology, 2020
The facultative methylotroph Bacillus methanolicus MGA3 has previously been genetically engineered to overproduce the amino acids L-lysine and L-glutamate and their derivatives cadaverine and γ-aminobutyric acid (GABA) from methanol at 50°C.
Sigrid Hakvåg, Ingemar Nærdal, Tonje M. B. Heggeset, Kåre A. Kristiansen, Inga M. Aasen, Trygve Brautaset   +5 more
doaj   +5 more sources

Charting the Metabolic Landscape of the Facultative Methylotroph Bacillus methanolicus [PDF]

MSystems, 2020
Delépine B, Gil Lopez M, Carnicer M, Vicente C, Wendisch VF, Heux S. Charting the metabolic landscape of the facultative methylotroph Bacillus methanolicus. mSystems.
Baudoin Delepine, Marina Gil Lopez, Marc Carnicer   +2 more
exaly   +10 more sources

Quantitative metabolomics of the thermophilic methylotroph Bacillus methanolicus. [PDF]

Microb Cell Fact, 2016
Background: The gram-positive bacterium Bacillus methanolicus MGA3 is a promising candidate for methanol-based biotechnologies. Accurate determination of intracellular metabolites is crucial for engineering this bacteria into an efficient microbial cell ...
Carnicer M, Vieira G, Brautaset T, Portais JC, Heux S.   +4 more
europepmc   +10 more sources

Harnessing the thermotolerant methylotroph Bacillus methanolicus for methanol-based synthetic L-proline production [PDF]

Microbial Cell Factories
Background Bacillus methanolicus MGA3 is a thermotolerant methylotroph that utilizes methanol, a renewable C₁ substrate, as its sole carbon and energy source.
Christine Frank, David Virant, Gregor Kosec, Tamara Hoffmann, Oskar Zelder, Max F. Felle, Erhard Bremer   +6 more
doaj   +6 more sources

Developing a Riboswitch-Mediated Regulatory System for Metabolic Flux Control in Thermophilic Bacillus methanolicus. [PDF]

Int J Mol Sci, 2021
Genome-wide transcriptomic data obtained in RNA-seq experiments can serve as a reliable source for identification of novel regulatory elements such as riboswitches and promoters. Riboswitches are parts of the 5′ untranslated region of mRNA molecules that
Irla M, Hakvåg S, Brautaset T.
europepmc   +7 more sources

Interrogating the Role of the Two Distinct Fructose-Bisphosphate Aldolases of Bacillus methanolicus by Site-Directed Mutagenesis of Key Amino Acids and Gene Repression by CRISPR Interference [PDF]

Frontiers in Microbiology, 2021
The Gram-positive Bacillus methanolicus shows plasmid-dependent methylotrophy. This facultative ribulose monophosphate (RuMP) cycle methylotroph possesses two fructose bisphosphate aldolases (FBA) with distinct kinetic properties.
Kerstin Schultenkämper, Desirée D. Gütle, Marina Gil López, Laura B. Keller, Lin Zhang, Oliver Einsle, Jean-Pierre Jacquot, Volker F. Wendisch   +7 more
doaj   +4 more sources

Development of Bacillus methanolicus methanol dehydrogenase with improved formaldehyde reduction activity [PDF]

Scientific Reports, 2018
Methanol dehydrogenase (MDH), an NAD+-dependent oxidoreductase, reversibly converts formaldehyde to methanol. This activity is a key step for both toxic formaldehyde elimination and methanol production in bacterial methylotrophy.
Jiyeun Yi, Jinhyuk Lee, Bong Hyun Sung, Du-Kyeong Kang, GyuTae Lim, Jung-Hoon Bae, Seung-Goo Lee, Sun Chang Kim, Jung-Hoon Sohn   +8 more
doaj   +5 more sources

Characterization of Fructose 1,6-Bisphosphatase and Sedoheptulose 1,7-Bisphosphatase from the Facultative Ribulose Monophosphate Cycle Methylotroph Bacillus methanolicus [PDF]

Journal of Bacteriology, 2013
Stolzenberger J, Lindner S, Persicke M, Brautaset T, Wendisch VF. Characterization of fructose 1,6-bisphosphatase and sedoheptulose 1,7-bisphosphatase from the facultative ribulose monophosphate cycle methylotroph Bacillus methanolicus.
Steffen N Lindner, Marcus Persicke, Trygve Brautaset   +2 more
exaly   +5 more sources

ADP-ribose is a competitive inhibitor of methanol dehydrogenases from Bacillus methanolicus. [PDF]

J Biol Chem
Methanol dehydrogenase (MDH), a representative of Type III alcohol dehydrogenases (ADHs), plays a pivotal role in methanol assimilation pathways, making it a key enzyme for the biosynthesis of chemicals and fuels from one-carbon feedstocks. An activator protein belonging to the Nudix hydrolase family, ACT, was found to increase the activity of MDH by ...
Ma BD, Li JY, Xu JH, Yu T, Kong XD.
europepmc   +4 more sources