Results 11 to 20 of about 115 (93)

Charting the Metabolic Landscape of the Facultative Methylotroph Bacillus methanolicus

mSystems, 2020
Bacillus methanolicus MGA3 is a thermotolerant and relatively fast-growing methylotroph able to secrete large quantities of glutamate and lysine. These natural characteristics make B.
Baudoin Delépine, Marina Gil López, Marc Carnicer, Cláudia M. Vicente, Volker F. Wendisch, Stéphanie Heux   +5 more
doaj   +6 more sources

Transaldolase in Bacillus methanolicus: biochemical characterization and biological role in ribulose monophosphate cycle [PDF]

BMC Microbiology, 2020
Background The Gram-positive facultative methylotrophic bacterium Bacillus methanolicus uses the sedoheptulose-1,7-bisphosphatase (SBPase) variant of the ribulose monophosphate (RuMP) cycle for growth on the C1 carbon source methanol.
Johannes Pfeifenschneider, Benno Markert, Jessica Stolzenberger, Trygve Brautaset, Volker F. Wendisch   +4 more
doaj   +3 more sources

Development of Bacillus methanolicus methanol dehydrogenase with improved formaldehyde reduction activity [PDF]

Scientific Reports, 2018
Methanol dehydrogenase (MDH), an NAD+-dependent oxidoreductase, reversibly converts formaldehyde to methanol. This activity is a key step for both toxic formaldehyde elimination and methanol production in bacterial methylotrophy.
Jiyeun Yi, Jinhyuk Lee, Bong Hyun Sung, Du-Kyeong Kang, GyuTae Lim, Jung-Hoon Bae, Seung-Goo Lee, Sun Chang Kim, Jung-Hoon Sohn   +8 more
doaj   +3 more sources

A synthetic biology toolkit for the plasmid-dependent and thermophilic methylotroph Bacillus methanolicus

Cell Reports
Summary: Bacillus methanolicus, a unique plasmid-dependent and thermophilic methylotroph, is an ideal chassis for one-carbon (C1) biomanufacturing. Despite its evolutionary uniqueness and industrial promise, the synthetic biology toolkit remains limited ...
Pan Liu, Qianqian Yuan, Xueting Yang, Qian Wang, Tao Chang, Yaning Bi, Peng Wu, Tong Zhang, Jinxing Yang, Shiting Guo, Chaoyou Xue, Zhaojuan Zheng, Bo Xin, Hongwu Ma, Yu Wang   +14 more
doaj   +3 more sources

Quantitative metabolomics of the thermophilic methylotroph Bacillus methanolicus [PDF]

Microbial Cell Factories, 2016
The gram-positive bacterium Bacillus methanolicus MGA3 is a promising candidate for methanol-based biotechnologies. Accurate determination of intracellular metabolites is crucial for engineering this bacteria into an efficient microbial cell factory.
Carnicer, Marc, Vieira, Gilles, Brautaset, Trygve, Portais, Jean-Charles, Heux, Stephanie   +4 more
openaire   +5 more sources

Plasmid-Dependent Methylotrophy in Thermotolerant Bacillus methanolicus [PDF]

Journal of Bacteriology, 2004
ABSTRACT Bacillus methanolicus can efficiently utilize methanol as a sole carbon source and has an optimum growth temperature of 50°C. With the exception of mannitol, no sugars have been reported to support rapid growth of this organism, which is classified as a restrictive methylotroph.
Trygve, Brautaset, Øyvind M, Jakobsen M, Michael C, Flickinger, Svein, Valla, Trond E, Ellingsen   +4 more
openaire   +2 more sources

Leveraging substrate flexibility and product selectivity of acetogens in two‐stage systems for chemical production

Microbial Biotechnology, Volume 16, Issue 2, Page 218-237, February 2023., 2023
Carbon dioxide recycling is a compelling need and microbial carbon dioxide fixation in value‐added compounds is a valuable opportunity. Fermentation of CO2 gas streams using acetogenic bacteria is consolidating as a key biotechnology to move toward a cyclic carbon economy.
Luca Ricci, Arne Seifert, Sebastien Bernacchi, Debora Fino, Candido Fabrizio Pirri, Angela Re   +5 more
wiley   +1 more source

Development and application of CRISPR‐based genetic tools in Bacillus species and Bacillus phages

Journal of Applied Microbiology, Volume 133, Issue 4, Page 2280-2298, October 2022., 2022
Abstract Recently, the clustered regularly interspaced short palindromic repeats (CRISPR) system has been developed into a precise and efficient genome editing tool. Since its discovery as an adaptive immune system in prokaryotes, it has been applied in many different research fields including biotechnology and medical sciences.
Yafeng Song, Siqi He, Anita Jopkiewicz, Rita Setroikromo, Ronald van Merkerk, Wim J. Quax   +5 more
wiley   +1 more source

Production of Value-Added Chemicals by Bacillus methanolicus Strains Cultivated on Mannitol and Extracts of Seaweed Saccharina latissima at 50°C

Frontiers in Microbiology, 2020
The facultative methylotroph Bacillus methanolicus MGA3 has previously been genetically engineered to overproduce the amino acids L-lysine and L-glutamate and their derivatives cadaverine and γ-aminobutyric acid (GABA) from methanol at 50°C.
Sigrid Hakvåg, Ingemar Nærdal, Tonje M. B. Heggeset, Kåre A. Kristiansen, Inga M. Aasen, Trygve Brautaset   +5 more
doaj   +1 more source

Efficient cell factories for the production of N‐methylated amino acids and for methanol‐based amino acid production

Microbial Biotechnology, Volume 15, Issue 8, Page 2145-2159, August 2022., 2022
Methanol is a reduced form of carbon dioxide that can be utilized by methylotrophic microorganisms. In this review, we review on how this feedstock, that does not have competing uses in food or feed, serves amino acid fermentation processes. Summary The growing world needs commodity amino acids such as L‐glutamate and L‐lysine for use as food and feed,
Marta Irla, Volker F. Wendisch
wiley   +1 more source