Results 1 to 10 of about 115 (93)

Metabolic engineering of thermophilic Bacillus methanolicus for riboflavin overproduction from methanol

Microbial Biotechnology, 2023
The growing need of next generation feedstocks for biotechnology spurs an intensification of research on the utilization of methanol as carbon and energy source for biotechnological processes.
Marta Irla
exaly   +5 more sources

Identification of MscS as a Key L‐Glutamate Exporter in Bacillus methanolicus

Microbial Biotechnology
Small‐conductance mechanosensitive channels (MscS) are established l‐glutamate exporters in industrially relevant bacteria, yet their role in the methylotrophic bacterium Bacillus methanolicus, a promising platform for sustainable methanol‐based l ...
Marta Irla
exaly   +7 more sources

Characterization of D-Arabitol as Newly Discovered Carbon Source of Bacillus methanolicus [PDF]

Frontiers in Microbiology, 2019
Bacillus methanolicus is a Gram-positive, thermophilic, methanol-utilizing bacterium. As a facultative methylotroph, B. methanolicus is also known to utilize D-mannitol, D-glucose and, as recently discovered, sugar alcohol D-arabitol.
Marina Gil Lopez, Marta Irla, Luciana Fernandes DE BRITO   +2 more
exaly   +6 more sources

Genomic and Transcriptomic Investigation of the Physiological Response of the Methylotroph Bacillus methanolicus to 5-Aminovalerate [PDF]

Frontiers in Microbiology, 2021
The methylotrophic thermophile Bacillus methanolicus can utilize the non-food substrate methanol as its sole carbon and energy source. Metabolism of L-lysine, in particular its biosynthesis, has been studied to some detail, and methanol-based L-lysine ...
Tobias Büsche, Carsten Haupka, Daniel Wibberg   +2 more
exaly   +5 more sources

CRISPR-Cas9-driven genome editing in Bacillus methanolicus MGA3

Frontiers in Microbiology
Bacillus methanolicus is a thermophilic methylotroph that uses methanol as a sustainable feedstock in biotechnological industry. Here, we developed the CRISPR-Cas9 genome editing tool for B. methanolicus MGA3.
Marina Gil Lopez, Trygve Brautaset, Jochen Schmid   +2 more
exaly   +6 more sources

Evaluation of Heterologous Biosynthetic Pathways for Methanol-Based 5-Aminovalerate Production by Thermophilic Bacillus methanolicus

Frontiers in Bioengineering and Biotechnology, 2021
The use of methanol as carbon source for biotechnological processes has recently attracted great interest due to its relatively low price, high abundance, high purity, and the fact that it is a non-food raw material.
Baudoin Delepine, Marta Irla, Simone Balzer Le   +2 more
exaly   +3 more sources

Development of a markerless tool for targeted chromosome modification in the thermophilic and methylotrophic bacterium Bacillus methanolicus

Microbial Cell Factories
Background Bacillus methanolicus is a promising candidate to become an industrial workhorse for methanol-based bioproduction due to its methylotrophy. However, genetic toolbox for this biotechnologically relevant thermophilic bacterium is still limited ...
Trygve Brautaset, Jochen Schmid, Volker F Wendisch   +2 more
exaly   +2 more sources

Genome-Based Genetic Tool Development for Bacillus methanolicus: Theta- and Rolling Circle-Replicating Plasmids for Inducible Gene Expression and Application to Methanol-Based Cadaverine Production

Frontiers in Microbiology, 2016
Bacillus methanolicus is a thermophilic methylotroph able to overproduce amino acids from methanol, a substrate not used for human or animal nutrition. Based on our previous RNA-seq analysis a mannitol inducible promoter and a putative mannitol activator
Marta Irla, Tonje Heggeset, Trygve Brautaset   +2 more
exaly   +3 more sources

Enhanced Glutamate Synthesis and Export by the Thermotolerant Emerging Industrial Workhorse Bacillus methanolicus in Response to High Osmolarity

Frontiers in Microbiology, 2021
The thermotolerant methylotroph Bacillus methanolicus MGA3 was originally isolated from freshwater marsh soil. Due to its ability to use methanol as sole carbon and energy source, B.
Christine Frank, Tamara Hoffmann, Tamara Hoffmann, Oskar Zelder, Max F. Felle, Erhard Bremer, Erhard Bremer   +6 more
doaj   +1 more source

Establishment of a functional system for recombinant production of secreted proteins at 50 °C in the thermophilic Bacillus methanolicus

Microbial Cell Factories, 2020
Background The suitability of bacteria as microbial cell factories is dependent on several factors such as price of feedstock, product range, production yield and ease of downstream processing.
Marta Irla, Eivind B. Drejer, Trygve Brautaset, Sigrid Hakvåg   +3 more
doaj   +1 more source