Characterization of D-Arabitol as Newly Discovered Carbon Source of Bacillus methanolicus [PDF]
Frontiers in Microbiology, 2019 Bacillus methanolicus is a Gram-positive, thermophilic, methanol-utilizing bacterium. As a facultative methylotroph, B. methanolicus is also known to utilize D-mannitol, D-glucose and, as recently discovered, sugar alcohol D-arabitol.
Marina Gil Lopez +2 more
exaly +11 more sources
Genomic and Transcriptomic Investigation of the Physiological Response of the Methylotroph Bacillus methanolicus to 5-Aminovalerate [PDF]
Frontiers in Microbiology, 2021 The methylotrophic thermophile Bacillus methanolicus can utilize the non-food substrate methanol as its sole carbon and energy source. Metabolism of L-lysine, in particular its biosynthesis, has been studied to some detail, and methanol-based L-lysine ...
Carsten Haupka +2 more
exaly +10 more sources
Development of a markerless tool for targeted chromosome modification in the thermophilic and methylotrophic bacterium Bacillus methanolicus [PDF]
Microbial Cell FactoriesBackground Bacillus methanolicus is a promising candidate to become an industrial workhorse for methanol-based bioproduction due to its methylotrophy. However, genetic toolbox for this biotechnologically relevant thermophilic bacterium is still limited ...
Marta Irla +2 more
exaly +8 more sources
Metabolic engineering of thermophilic Bacillus methanolicus for riboflavin overproduction from methanol [PDF]
Microbial Biotechnology, 2023 The growing need of next generation feedstocks for biotechnology spurs an intensification of research on the utilization of methanol as carbon and energy source for biotechnological processes.
Vivien Jessica Klein +4 more
doaj +7 more sources
Identification of MscS as a Key L‐Glutamate Exporter in Bacillus methanolicus [PDF]
Microbial BiotechnologySmall‐conductance mechanosensitive channels (MscS) are established l‐glutamate exporters in industrially relevant bacteria, yet their role in the methylotrophic bacterium Bacillus methanolicus, a promising platform for sustainable methanol‐based l ...
Luciana Fernandes Brito +5 more
doaj +9 more sources
Transaldolase in Bacillus methanolicus: biochemical characterization and biological role in ribulose monophosphate cycle [PDF]
BMC Microbiology, 2020 Background The Gram-positive facultative methylotrophic bacterium Bacillus methanolicus uses the sedoheptulose-1,7-bisphosphatase (SBPase) variant of the ribulose monophosphate (RuMP) cycle for growth on the C1 carbon source methanol.
Johannes Pfeifenschneider +4 more
doaj +7 more sources
Evaluation of Heterologous Biosynthetic Pathways for Methanol-Based 5-Aminovalerate Production by Thermophilic Bacillus methanolicus [PDF]
Frontiers in Bioengineering and Biotechnology, 2021 The use of methanol as carbon source for biotechnological processes has recently attracted great interest due to its relatively low price, high abundance, high purity, and the fact that it is a non-food raw material.
Luciana Fernandes Brito +2 more
exaly +9 more sources
Establishment of a functional system for recombinant production of secreted proteins at 50 °C in the thermophilic Bacillus methanolicus [PDF]
Microbial Cell Factories, 2020 Background The suitability of bacteria as microbial cell factories is dependent on several factors such as price of feedstock, product range, production yield and ease of downstream processing.
Marta Irla +3 more
doaj +5 more sources
Genome-Based Genetic Tool Development for Bacillus methanolicus: Theta- and Rolling Circle-Replicating Plasmids for Inducible Gene Expression and Application to Methanol-Based Cadaverine Production [PDF]
Frontiers in Microbiology, 2016 Bacillus methanolicus is a thermophilic methylotroph able to overproduce amino acids from methanol, a substrate not used for human or animal nutrition. Based on our previous RNA-seq analysis a mannitol inducible promoter and a putative mannitol activator
Marta Irla +2 more
exaly +4 more sources
Enhanced Glutamate Synthesis and Export by the Thermotolerant Emerging Industrial Workhorse Bacillus methanolicus in Response to High Osmolarity [PDF]
Frontiers in Microbiology, 2021 The thermotolerant methylotroph Bacillus methanolicus MGA3 was originally isolated from freshwater marsh soil. Due to its ability to use methanol as sole carbon and energy source, B.
Christine Frank +6 more
doaj +2 more sources