Results 21 to 30 of about 115 (93)

Integrating greenhouse gas capture and C1 biotechnology: a key challenge for circular economy

Microbial Biotechnology, Volume 15, Issue 1, Page 228-239, January 2022., 2022
State of the art on the valorisation of C1 carbon sources obtained either from natural or anthropogenic origins as a key challenge for the circular economy.
José L. García, Beatriz Galán
wiley   +1 more source

Characterization of a restriction-modification system of the thermotolerant methylotroph Bacillus methanolicus [PDF]

Applied and Environmental Microbiology, 1996
We report the isolation of a restriction endonuclease, BmeTI, an isoschizomer of BclI, that recognizes the DNA sequence 5' TGATCA 3'. We also report that BmeTI sites are modified to TGm6ATCA. These findings provide the basis for devising strategies to prevent BmeTI restriction of any DNA introduced into Bacillus methanolicus.
D, Cue, H, Lam, R S, Hanson, M C, Flickinger   +3 more
openaire   +2 more sources

Metabolic engineering of microorganisms for the production of multifunctional non‐protein amino acids: γ‐aminobutyric acid and δ‐aminolevulinic acid

Microbial Biotechnology, Volume 14, Issue 6, Page 2279-2290, November 2021., 2021
For the first time, we put GABA and ALA together for a detailed summary, analysis and comparison, and put forward further strategies to meet the challenges of industrial competition strains. Summary Gamma‐aminobutyric acid (GABA) and delta‐aminolevulinic acid (ALA), playing important roles in agriculture, medicine and other fields, are multifunctional ...
Anping Su, Qijun Yu, Ying Luo, Jinshui Yang, Entao Wang, Hongli Yuan   +5 more
wiley   +1 more source

Genetic manipulation of Bacillus methanolicus, a gram-positive, thermotolerant methylotroph [PDF]

Applied and Environmental Microbiology, 1997
We report the fist genetic transformation system, shuttle vectors, and integrative vectors for the thermotolerant, methylotrophic bacterium Bacillus methanolicus. By using a polyethylene glycol-mediated transformation procedure, we have successfully transformed B. methanolicus with both integrative and multicopy plasmids.
D, Cue, H, Lam, R L, Dillingham, R S, Hanson, M C, Flickinger   +4 more
openaire   +2 more sources

Strategies and challenges with the microbial conversion of methanol to high‐value chemicals

Biotechnology and Bioengineering, Volume 118, Issue 10, Page 3655-3668, October 2021., 2021
Methanol is considered as one promising alternative substrate for cell refinery, owing to its availability from a variety of sources (CO2, petroleum, nature gas, coal). Here we present a detailed analysis of methanol metabolic pathways from different methylotrophs.
Chunjun Zhan, Xiaowei Li, Yankun Yang, Jens Nielsen, Zhonghu Bai, Yun Chen   +5 more
wiley   +1 more source

Phage-Assisted Evolution of Bacillus methanolicus Methanol Dehydrogenase 2 [PDF]

ACS Synthetic Biology, 2019
Synthetic methylotrophy, the modification of organisms such as E. coli to grow on methanol, is a longstanding goal of metabolic engineering and synthetic biology. The poor kinetic properties of NAD-dependent methanol dehydrogenase, the first enzyme in most methanol assimilation pathways, limit pathway flux and present a formidable challenge to ...
Timothy B. Roth, Benjamin M. Woolston, Gregory Stephanopoulos, David R. Liu   +3 more
openaire   +4 more sources

Transcriptome analysis reveals the roles of nitrogen metabolism and sedoheptulose bisphosphatase pathway in methanol‐dependent growth of Corynebacterium glutamicum

Microbial Biotechnology, Volume 14, Issue 4, Page 1797-1808, July 2021., 2021
Transcriptome analysis of methanol‐dependent Corynebacterium glutamicum reveals metabolic regulation changes in central metabolism. It is experimentally verified that nitrate could serve as an additional electron acceptor for aerobic methanol metabolism and the biosynthesis of several amino acids limits methylotrophic growth, and the SBPase variant for
Liwen Fan, Yu Wang, Jin Qian, Ning Gao, Zhihui Zhang, Xiaomeng Ni, Letian Sun, Qianqian Yuan, Ping Zheng, Jibin Sun   +9 more
wiley   +1 more source

Methylotrophic Bacillus methanolicus encodes two chromosomal and one plasmid born NAD+ dependent methanol dehydrogenase paralogs with different catalytic and biochemical properties. [PDF]

PLoS ONE, 2013
Bacillus methanolicus can utilize methanol as the sole carbon source for growth and it encodes an NAD(+)-dependent methanol dehydrogenase (Mdh), catalyzing the oxidation of methanol to formaldehyde. Recently, the genomes of the B.
Anne Krog, Tonje M B Heggeset, Jonas E N Müller, Christiane E Kupper, Olha Schneider, Julia A Vorholt, Trond E Ellingsen, Trygve Brautaset   +7 more
doaj   +1 more source

Growth of Bacillus methanolicus in seawater-based media

Journal of Industrial Microbiology & Biotechnology, 2005
Bacillus methanolicus has been proposed as a biocatalyst for the low cost production of commodity chemicals. The organism can use methanol as sole carbon and energy source, and it grows aerobically at elevated temperatures. Methanol can be made available from off-shore conversion of natural gas to methanol, through gas-to-liquid technology.
Claire F, Komives, Louis Yip-Yan, Cheung, Stefanie B, Pluschkell, Michael C, Flickinger   +3 more
openaire   +2 more sources

Methanol-based acetoin production by genetically engineered Bacillus methanolicus [PDF]

Green Chemistry, 2020
Establishment of sustainable technology for methanol-based production of acetoin by metabolically engineered Bacillus methanolicus .
Eivind B. Drejer, Dennis Tin Chat Chan, Carsten Haupka, Volker F. Wendisch, Trygve Brautaset, Marta Irla   +5 more
openaire   +3 more sources