Genetic manipulation of Bacillus methanolicus, a gram-positive, thermotolerant methylotroph [PDF]
Applied and Environmental Microbiology, 1997 We report the fist genetic transformation system, shuttle vectors, and integrative vectors for the thermotolerant, methylotrophic bacterium Bacillus methanolicus. By using a polyethylene glycol-mediated transformation procedure, we have successfully transformed B. methanolicus with both integrative and multicopy plasmids.
David Cue +4 more
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Methanol-based cadaverine production by genetically engineered Bacillus methanolicus strains. [PDF]
Microb Biotechnol, 2019 Naerdal I, Pfeifenschneider J, Brautaset T, Wendisch VF. Erratum: Methanol-based cadaverine production by genetically engineered Bacillus methanolicus strains. Microbial Biotechnology.
Wendisch, Volker F. ; https://orcid.org/ +3 more
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Characterization of a restriction-modification system of the thermotolerant methylotroph Bacillus methanolicus [PDF]
Applied and Environmental Microbiology, 1996 We report the isolation of a restriction endonuclease, BmeTI, an isoschizomer of BclI, that recognizes the DNA sequence 5' TGATCA 3'. We also report that BmeTI sites are modified to TGm6ATCA. These findings provide the basis for devising strategies to prevent BmeTI restriction of any DNA introduced into Bacillus methanolicus.
David Cue +3 more
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Growth of Bacillus methanolicus in seawater-based media [PDF]
Journal of Industrial Microbiology & Biotechnology, 2005 Bacillus methanolicus has been proposed as a biocatalyst for the low cost production of commodity chemicals. The organism can use methanol as sole carbon and energy source, and it grows aerobically at elevated temperatures. Methanol can be made available from off-shore conversion of natural gas to methanol, through gas-to-liquid technology.
Claire Komives +3 more
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Corrigendum: Genome-Based Genetic Tool Development for Bacillus methanolicus: Theta- and Rolling Circle-Replicating Plasmids for Inducible Gene Expression and Application to Methanol-Based Cadaverine Production [PDF]
Frontiers in Microbiology, 2019 Marta Irla +8 more
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Phage-Assisted Evolution of Bacillus methanolicus Methanol Dehydrogenase 2. [PDF]
ACS Synth Biol, 2019 Synthetic methylotrophy, the modification of organisms such as E. coli to grow on methanol, is a longstanding goal of metabolic engineering and synthetic biology. The poor kinetic properties of NAD-dependent methanol dehydrogenase, the first enzyme in most methanol assimilation pathways, limit pathway flux and present a formidable challenge to ...
Roth TB +3 more
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J Inorg Biochem, 2018 The CYP152 family of cytochrome P450 enzymes (P450s or CYPs) are bacterial peroxygenases that use hydrogen peroxide to drive hydroxylation and decarboxylation of fatty acid substrates. We have expressed and purified a novel CYP152 family member - CYP152K6 from the methylotroph Bacillus methanolicus MGA3.
Girvan HM +7 more
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Role of the Bacillus methanolicus Citrate Synthase II Gene, citY , in Regulating the Secretion of Glutamate in l -Lysine-Secreting Mutants [PDF]
Applied and Environmental Microbiology, 2003 Trygve Brautaset, Michael C Flickinger
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Plasmid-dependent methylotrophy in thermotolerant Bacillus methanolicus. [PDF]
J Bacteriol, 2004 ABSTRACT Bacillus methanolicus can efficiently utilize methanol as a sole carbon source and has an optimum growth temperature of 50°C. With the exception of mannitol, no sugars have been reported to support rapid growth of this organism, which is classified as a restrictive methylotroph.
Brautaset T +4 more
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Methylotrophic Bacillus methanolicus encodes two chromosomal and one plasmid born NAD+ dependent methanol dehydrogenase paralogs with different catalytic and biochemical properties. [PDF]
PLoS ONE, 2013 Bacillus methanolicus can utilize methanol as the sole carbon source for growth and it encodes an NAD(+)-dependent methanol dehydrogenase (Mdh), catalyzing the oxidation of methanol to formaldehyde. Recently, the genomes of the B.
Anne Krog +7 more
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