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Trends in Microbiology, 2019
The pBR322 plasmid was used for constructing pLBl plasmid carrying genes of Bacillus subtills lysine biosynthesis. B. subtilis and E. coli strains (lysine-auxotrophs) are transformed. The plasmid is shown to complement both mutations in seven genes of lysine biosynthesis in E. coli cells and four mutations in B. subtills.
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The pBR322 plasmid was used for constructing pLBl plasmid carrying genes of Bacillus subtills lysine biosynthesis. B. subtilis and E. coli strains (lysine-auxotrophs) are transformed. The plasmid is shown to complement both mutations in seven genes of lysine biosynthesis in E. coli cells and four mutations in B. subtills.
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Sporulation of Bacillus subtilis
Current Opinion in Microbiology, 2004Differentiation of vegetative Bacillus subtilis into heat resistant spores is initiated by the activation of the key transcription regulator Spo0A through the phosphorelay. Subsequent events depend on the cell compartment-specific action of a series of RNA polymerase sigma factors.
Patrick J, Piggot, David W, Hilbert
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Cytochromes of Bacillus megaterium and Bacillus subtilis
Archives of Biochemistry and Biophysics, 1960Abstract Treatment of Bacillus subtilis and Bacillus megaterium cells by ordinary procedures failed to extract any soluble cytochrome from the cell. Sonoration produced small fragments which contained the cytochrome complement and were sedimentable in a centrifugal field of 144,000 × g .
L P, VERNON, J H, MANGUM
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Transformation of Bacillus subtilis
2014Bacillus subtilis has tremendous applications in both academic research and industrial production. However, molecular cloning and transformation of B. subtilis are not as easy as those of Escherichia coli. Here we developed a simple protocol based on super-competent cells prepared from the recombinant B.
Xiao-Zhou, Zhang +2 more
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Genetic mapping in Bacillus subtilis
Journal of Molecular Biology, 1967Abstract A map of the Bacillus subtilis genome has been constructed from the results of density transfer experiments carried out in strain W23, and from genetic linkage data obtained using DNA-mediated transformation and phage PBS1-mediated transduction in strain 168. Several new linkages have been detected and most of the known linkage groups have
D, Dubnau +3 more
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Ultrastructural cytochemistry of Bacillus subtilis
Journal of Ultrastructure Research, 1967The ultrastructure of B. subtilis has been studied with a new procedure of fixation with glutaraldehyde and embedding in glycol-methacrylate (GMA) because cytochemical studies can be carried out directly on the ultrathin sections. The cell wall appears triple-layered with two dense layers enclosing a less dense one.
P, Granboulan, E H, Leduc
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Autoradiography of the Bacillus subtilis chromosome
Journal of Molecular Biology, 1966The DNA content of the Bacillus subtilis spore has been redetermined and found to agree with the previous estimate by Fitz-James & Young (1959) . This sets an upper limit of 3·0 × 10 9 daltons (1700 μ of the B form) as the amount of DNA in the B. subtilis genome. By autoradiography continuous chromosomal lengths of 800 to 900 μ are shown to occur
E S, Dennis, R G, Wake
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Expansion Microscopy of Bacillus subtilis
2022Expansion microscopy enables super-resolved visualization of specimen without the need of highly sophisticated and expensive optical instruments. Instead, the method is executed with conventional chemicals and lab equipment. Imaging of bacteria is performed using standard fluorescence microscopy.
Viola, Middelhauve +2 more
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The effect of carbamates on Bacillus subtilis
Mutation Research, 1967Abstract Ethyl carbamate and several structurally related compounds were studied to determine their relative phenotypic and genotypic effects on Bacillus subtilis. In general, bacterial growth inhibition noted was increased by the addition of larger radicals and larger radicals with branching to the carboethoxy group of the molecule; in addition, a ...
R, de Giovanni-Donnelly +2 more
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Shape determination in Bacillus subtilis
Current Opinion in Microbiology, 2007The discovery of cytoskeletal elements in prokaryotes has dramatically changed the way we think about bacterial cell morphogenesis. The rod shape of Bacillus subtilis is maintained by the two major polymers (peptidoglycan and teichoic acids) of its thick cell wall and determined by the way these are inserted during growth.
Rut, Carballido-López, Alex, Formstone
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