Results 91 to 100 of about 1,491,725 (269)

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

FEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee, Ga Seul Lee, JinHan Kim, Kwang‐Hyun Park, Seong‐Ryung Go, Jeong Hee Moon, Eui‐Jeon Woo   +6 more
wiley   +1 more source

Molecular Insights and Phylogenetic Analysis of Acinetobacter baumannii Carrying Carbapenem Resistance Genes in Broiler Chickens: An Emerging Threat to Human Health

Bacteria
Acinetobacter baumannii remains a significant nosocomial infectious agent, with its ability to acquire antimicrobial resistance posing a global public health concern. Over time, substantial knowledge has been amassed regarding A.
Hala M. Zaher
doaj   +1 more source

Identifying transcription factors controlling the basal expression of human MRP4 highlights a substantial role for Sp1

FEBS Open Bio, EarlyView.
The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer, Angelique Kragl, Katrin Ziems, Juliane Glaubitz, Sophie Grammbauer, Susanne Hildebrandt, Mladen V. Tzvetkov, Gabriele Jedlitschky   +7 more
wiley   +1 more source

Unveiling and Assessing Antibiotic Misuse in Saudi Arabia—A Systematic Review and Meta-Analysis

Bacteria
Background: Antibiotic misuse and prescribing errors are significant concerns in clinical practice, contributing to unnecessary antibiotic exposure, increased adverse effects, rising healthcare costs, and the escalation of antibiotic resistance ...
Reena Almutairi, Rawan Alharbi, Lamar Alfuraydi, Yousef Alawi, Nouralhoda Mourabya, Hiba Shokry, Ammar Ramli, Idris Sula, Hany Lotfy, Mateq Ali Alreshidi   +9 more
doaj   +1 more source

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

FEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata, Yoichi Sakai, Keisuke Tanaka, Etsuro Yoshimura, Ken Kitano, Morio Ishikawa, Tadahiro Nakamoto, Tsutomu Takayama, Hirofumi Inoue, Kaho Nomura, Tomonori Suzuki, Masataka Uchino, Akira Abe, Youichi Niimura   +13 more
wiley   +1 more source

Shiga Toxin-Producing Escherichia coli in Poultry: Virulence, Antimicrobial Resistance, and Zoonotic Implications

Bacteria
Shiga toxin-producing Escherichia coli (STEC) is a major zoonotic foodborne pathogen associated with severe human illnesses, including hemorrhagic colitis and hemolytic uremic syndrome.
Arjmand Fatima, Muhammad Naeem
doaj   +1 more source

Lipopolysaccharide uptake is augmented in lipopolysaccharide‐tolerant mouse macrophage‐like cells via increased CD14 expression

FEBS Open Bio, EarlyView.
In normal (nontolerant) cells, CD14 is crucial for both LPS uptake and LPS signaling. In LPS‐tolerant cells, in which LPS‐induced TNF‐α and IFN‐β production is suppressed, there is a dramatic increase in surface CD14 expression. The overexpressed CD14 in LPS‐tolerant cells is responsible for the enhanced LPS uptake without inducing pro‐inflammatory ...
Saeka Nishihara, Takayuki Manabe, Mika Jouta, Kiyoshi Kawasaki   +3 more
wiley   +1 more source

Influence of Infection Origin, Type of Sampling, and Weather Factors on the Periodicity of Some Infectious Pathogens in Marseille University Hospitals, France

Bacteria
This study aimed at systematically exploring the seasonalities of bacterial identifications from 1 February 2014 to 31 January 2020 in hospitalized patients, considering the infectious site and the community-acquired or hospital-associated origin ...
Lanceï Kaba, Audrey Giraud-Gatineau, Philippe Colson, Pierre-Edouard Fournier, Hervé Chaudet   +4 more
doaj   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Molecular Characterization of Phosphate Solubilizing Bacteria Klebsiella variicola PSEG-1 Associated with Aporrectodea rosea Gastrointestinal Tract

Bacteria
Phosphorus is a macronutrient crucially important for plant growth and development; its limited amount in soil and water poses bewildering concerns amongst agronomists.
Vikash Kerketta, Amrita Kumari Panda, Aseem Kerketta, Surajit De Mandal, Satpal Singh Bisht   +4 more
doaj   +1 more source