Results 81 to 90 of about 1,491,725 (269)

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

Mechanisms Underpinning the Biocontrol Potential of Halophilic and Halotolerant Bacillus Species Against Fusarium and Other Fungal Phytopathogens: An Eco-Friendly Alternative for Sustainable Agriculture

Bacteria
Modern agriculture is increasingly challenged by fungal diseases, with phytopathogens such as Fusarium species causing substantial yield and quality losses in major crops globally.
Leyla Miloudi-Agha, Mohammed Kebdani
doaj   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Survival of Bacterial Pathogens During Storage of Animal Waste and Wastewater Treatment Sludge and Their Subsequent Application to Clay–Loam Soil

Bacteria
Globally, large quantities of animal waste and human sewage sludge are generated annually. Their application as soil amendments can enhance soil quality and support a circular economy.
Natalia Alija-Novo, Paul Whyte, Declan Bolton   +2 more
doaj   +1 more source

Combined 5‐aminolevulinic acid and ferric ammonium citrate treatment promotes hair follicle growth by activating dermal papilla cells

FEBS Open Bio, EarlyView.
5‐Aminolevulinic acid combined with ferric ammonium citrate (5‐ALA/FAC) stimulates dermal papilla cell activity and promotes hair follicle growth. The treatment enhances ERK and AKT signaling, increases hair‐inductive gene expression, and restores dermal papilla function suppressed by dihydrotestosterone and oxidative stress, resulting in enhanced hair
Han‐Wook Ryu, Eok‐Soo Oh, Sewoon Kim
wiley   +1 more source

Nomenclatural Recommendations for Genera Assigned to the Arcobacteraceae from the Subcommittee on the Taxonomy of Campylobacter and Related Bacteria

Bacteria
The taxonomy of the genus Arcobacter has been subject to substantive turmoil in recent years following a proposal to subdivide the genus into six genera.
Stephen L. W. On, Maria J. Figueras, James G. Fox, Kurt Houf, Francis Mégraud, William G. Miller, John Stolz, Ken Takai, Peter Vandamme   +8 more
doaj   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

Advanced Sequencing Approaches for the Subgingival Microbiome: Technology Selection, Quality Control, and Best Practices in Periodontal Research

Bacteria
Sequencing technologies have reshaped the study of the subgingival microbiome, but selecting the appropriate method remains challenging because of differences in resolution, cost, host DNA contamination, and computational complexity. This review compares
Hadeel Mazin Akram, Saif Sehaam Saliem
doaj   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Targeting Drug-Resistant Pseudomonas aeruginosa: Emerging Roles of Plant-Derived Bioactive Compounds

Bacteria
Pseudomonas aeruginosa, an opportunistic pathogen, is a major threat to hospital infection control and global public health due to its strong environmental adaptability, complex virulence systems, efficient biofilm formation capability, and widespread ...
Jing Feng, Dandan Liu, Wei Xiao, Peijie Fu, Juanli Cheng, Jinshui Lin   +5 more
doaj   +1 more source