Results 51 to 60 of about 1,488,259 (274)

Delivery of genome editing tools by bacterial extracellular vesicles [PDF]

Microbial Biotechnology, 2018
Here we propose to use bacterial cells as factories that generate EVs harboring both the RNP complex (or any other CRISPR-mediated tool) and a specific ligand molecule. The ligand would allow specific binding of EVs to cells with the matching receptors, adding a level of specificity to the delivery, hence stimulating precise genome editing.
Liu, Yue, Smid, Eddy J., Abee, Tjakko, Notebaart, Richard A.   +3 more
openaire   +3 more sources

Dual roles of the conditional extracellular vesicles derived from Pseudomonas aeruginosa biofilms: Promoting and inhibiting bacterial biofilm growth

Biofilm
Antibiotic-resistant biofilm infections have emerged as public health concerns because of their enhanced tolerance to high-dose antibiotic treatments. The biofilm life cycle involves multiple developmental stages, which are tightly regulated by active ...
Marwa Gamal Saad, Haluk Beyenal, Wen-Ji Dong   +2 more
doaj   +1 more source

The microbiome in human skin aging

FEBS Letters, EarlyView.
Age‐related skin changes encompass the well‐known visible phenotypic alterations, together with microbiome dysbiosis and a series of molecular aging hallmarks. These hallmarks characterize not only a fully stablished aged phenotype but also the skin aging process itself.
Manuel Huerta Arana, Cornelia Wiegand, Omid Omrani, Mario Fabri   +3 more
wiley   +1 more source

The International Society for Extracellular Vesicles launches the first massive open online course on extracellular vesicles [PDF]

, 2016
The International Society for Extracellular Vesicles (ISEV) has organised its first educational online course for students and beginners in the field of extracellular vesicles (EVs). This course, “Basics of Extracellular Vesicles,” uses recorded lectures
Lötvall, Jan,, Lötvall, Jan, Mathivanan, Suresh, Lässer, Cecilia, Théry, Clotilde, Buzás, E.I., Mathivanan, S., Lötval, J., Buzás, Edit I, Yong Song Gho, Lässer, C., Zhao, Weian, Gho, Yong Song, Zhao, W., Gho, Yong Song,, Edit I. Buzás, Jan Lötvall, Lässer, Cecilia,, Gho, Y.S., Suresh Mathivanan, Weian Zhao, Théry, C., Cecilia Lässer, Clotilde Théry   +23 more
core   +1 more source

Structural and biochemical analysis of a B12 superbinder

FEBS Letters, EarlyView.
BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices, Jacob J. Whittaker, Dirk J. Slotboom, Albert Guskov   +3 more
wiley   +1 more source

Escherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes

mBio, 2023
Extracellular vesicles are considered to be an inflammatory factor in several acute and chronic inflammatory diseases. The present study shows that exosomes from macrophages (Mφ) infected with live Escherichia coli induced secretion of proinflammatory ...
Risa Imamiya, Akari Shinohara, Daisuke Yakura, Takehiro Yamaguchi, Koji Ueda, Ami Oguro, Yukiko Minamiyama, Hiroshi Ichikawa, Yasuhiko Horiguchi, Mayuko Osada-Oka   +9 more
doaj   +1 more source

Brain endothelium-derived extracellular vesicles containing amyloid-beta induce mitochondrial alterations in neural progenitor cells

, 2022
Aim: Elevated brain deposits of amyloid beta (Aβ40) contribute to neuropathology and cognitive dysfunction in Alzheimer’s disease (AD). However, the role of the blood-brain barrier (BBB) as an interface for the transfer of Aβ40 from the periphery into ...
Kowalczyk, Jennifer M, Louis, Kelssey D Pierre, Daftari, Manav T, Dykxhoorn, Derek M, Daftari, Manav T., Colbert, Brett M., Toborek, Michal, Kowalczyk, Jennifer M., András, Ibolya E., Dykxhoorn, Derek M., Colbert, Brett M, Torices, Silvia, Pierre Louis, Kelssey D., Osborne, Olivia M, András, Ibolya E, Naranjo, Oandy, Osborne, Olivia M.   +16 more
core   +1 more source

Metastatic niche shaped by host factors influences disseminated cancer cell fate

FEBS Letters, EarlyView.
Metastasis is shaped not only by cancer cells but also by the environments they encounter. This review explores how factors such as aging, diet, the microbiome, lifestyle, and environmental exposures remodel organ‐specific niches in the lung, liver, bone, and brain, influencing where metastatic cells survive, remain dormant, or grow, and ultimately ...
Gwennan Delyth Ward, Álvaro Ruiz‐Tabas, Patricia Altea‐Manzano   +2 more
wiley   +1 more source

DNA ligase LigE increases bacterial transformation with damaged extracellular DNA

FEBS Letters, EarlyView.
The DNA ligase LigE from the naturally competent Gram‐negative bacterium Neisseria gonorrhoaea increases transformation with cut extracellular DNA. This process is ATP‐dependent and relies on the catalytic activity of LigE; it also requires that LigE contains an intact periplasmic localisation sequence, indicating that ligE functions in the periplasm ...
Jolyn Pan, Avi Singh, Shrawani Weralupe Gamladdalage, Joanna Hicks, Adele Williamson   +4 more
wiley   +1 more source

Extracellular vesicles from microalgae

, 2021
The invention relates to extracellular vesicles derived from native, photosynthetic, non-fermenting microalgae, to a method of isolating extracellular vesicles from native, photosynthetic, non-fermenting microalgae by growth, centrifugation and ...
Gabriella Pocsfalvi, Nicolas Touzet, Mauro Manno, Antonella Bongiovanni   +3 more
core