Results 61 to 70 of about 920,931 (266)

Transposon Insertion Sequencing Elucidates Novel Gene Involvement in Susceptibility and Resistance to Phages T4 and T7 in Escherichia coli O157

mBio, 2018
Experiments using bacteriophage (phage) to infect bacterial strains have helped define some basic genetic concepts in microbiology, but our understanding of the complexity of bacterium-phage interactions is still limited.
Lauren A. Cowley, Alison S. Low, Derek Pickard, Christine J. Boinett, Timothy J. Dallman, Martin Day, Neil Perry, David L. Gally, Julian Parkhill, Claire Jenkins, Amy K. Cain   +10 more
doaj   +1 more source

Population genomics and antimicrobial resistance in Corynebacterium diphtheriae

Genome Medicine, 2020
Background Corynebacterium diphtheriae, the agent of diphtheria, is a genetically diverse bacterial species. Although antimicrobial resistance has emerged against several drugs including first-line penicillin, the genomic determinants and population ...
Melanie Hennart, Leonardo G. Panunzi, Carla Rodrigues, Quentin Gaday, Sarah L. Baines, Marina Barros-Pinkelnig, Annick Carmi-Leroy, Melody Dazas, Anne Marie Wehenkel, Xavier Didelot, Julie Toubiana, Edgar Badell, Sylvain Brisse   +12 more
doaj   +1 more source

Identifying transcription factors controlling the basal expression of human MRP4 highlights a substantial role for Sp1

FEBS Open Bio, EarlyView.
The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer, Angelique Kragl, Katrin Ziems, Juliane Glaubitz, Sophie Grammbauer, Susanne Hildebrandt, Mladen V. Tzvetkov, Gabriele Jedlitschky   +7 more
wiley   +1 more source

Tail assembly interference is a common strategy in bacterial antiviral defenses

Nature Communications
Many bacterial immune systems recognize phage structural components to activate antiviral responses, without inhibiting the function of the phage component.
Lingchen He, Laura Miguel-Romero, Jonasz B. Patkowski, Nasser Alqurainy, Eduardo P. C. Rocha, Tiago R. D. Costa, Alfred Fillol-Salom, José R. Penadés   +7 more
doaj   +1 more source

Lipopolysaccharide uptake is augmented in lipopolysaccharide‐tolerant mouse macrophage‐like cells via increased CD14 expression

FEBS Open Bio, EarlyView.
In normal (nontolerant) cells, CD14 is crucial for both LPS uptake and LPS signaling. In LPS‐tolerant cells, in which LPS‐induced TNF‐α and IFN‐β production is suppressed, there is a dramatic increase in surface CD14 expression. The overexpressed CD14 in LPS‐tolerant cells is responsible for the enhanced LPS uptake without inducing pro‐inflammatory ...
Saeka Nishihara, Takayuki Manabe, Mika Jouta, Kiyoshi Kawasaki   +3 more
wiley   +1 more source

A silent network’s resounding success: how mutations of core metabolic genes confer antibiotic resistance

Signal Transduction and Targeted Therapy, 2021
Gamal Wareth, Heinrich Neubauer, Lisa D. Sprague   +2 more
doaj   +1 more source

Alterations of Gut Microbiome in the Patients With Severe Fever With Thrombocytopenia Syndrome

Frontiers in Microbiology, 2018
Severe fever with thrombocytopenia syndrome (SFTS) is an emerging tick-borne infectious disease caused by SFTS virus, and the number of SFTS cases increased year by year in China. Previous studies had indicated that gut microbiome closely associated with
Honghai Xu, Honghai Xu, Honghai Xu, Yuanyuan Wei, Yuanyuan Wei, Hongqiu Ma, Yanyan Liu, Yalong Zhang, Lifen Hu, Lifen Hu, Jiabin Li, Jiabin Li   +11 more
doaj   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Time‐restricted feeding prior to Mycobacterium tuberculosis infection reduces tissue CD4+ T cells with limited impact on bacterial clearance

FEBS Open Bio, EarlyView.
Time‐restricted feeding (TRF) in mice increased liver fatty acid oxidation and decreased fatty acid biosynthesis. These alterations persisted when TRF was discontinued and the host was infected with Mycobacterium tuberculosis. Pre‐exposure to TRF did not alter tissue (lung and spleen) mycobacterial burden but significantly reduced CD3+ T cells in lungs
Ashish Gupta, Nidhi Yadav, Subhasmita Das, R. Rajendra Kumar Reddy, Nupur Sharma, Amol Ratnakar Suryawanshi, Jaswinder Singh Maras, Ranjan Kumar Nanda   +7 more
wiley   +1 more source

Assessing Membrane Fluidity and Visualizing Fluid Membrane Domains in Bacteria Using Fluorescent Membrane Dyes

Bio-Protocol, 2018
Membrane fluidity is a key parameter of bacterial membranes that undergoes quick adaptation in response to environmental challenges and has recently emerged as an important factor in the antibacterial mechanism of membrane-targeting antibiotics.
Michaela Wenzel, Norbert Vischer, Henrik Strahl, Leendert Hamoen   +3 more
doaj   +1 more source