Results 101 to 110 of about 3,299,929 (298)

Bacterial flagellar capping proteins adopt diverse oligomeric states

eLife, 2016
Flagella are crucial for bacterial motility and pathogenesis. The flagellar capping protein (FliD) regulates filament assembly by chaperoning and sorting flagellin (FliC) proteins after they traverse the hollow filament and exit the growing flagellum tip.
Sandra Postel, Daniel Deredge, Daniel A Bonsor, Xiong Yu, Kay Diederichs, Saskia Helmsing, Aviv Vromen, Assaf Friedler, Michael Hust, Edward H Egelman, Dorothy Beckett, Patrick L Wintrode, Eric J Sundberg   +12 more
doaj   +1 more source

From junk to function — How weak selection in eukaryotes builds new parts and drives genomic complexity

FEBS Letters, EarlyView.
How do genomes gain new functional parts? In eukaryotes, which tend to evolve under weak selection, much of the genome is junk. Palazzo and Qiu borrow the logic of Markov chains to show how non‐functional DNA becomes functional through the appearance of intermediate states, which arise due to epistasis, buffering, and biochemical messiness, allowing ...
Alexander F. Palazzo, Yi Qiu
wiley   +1 more source

Dimeric structure of the bacterial extracellular foldase PrsA [PDF]

, 2015
Secretion of proteins into the membrane-cell wall space is essential for cell wall biosynthesis and pathogenicity in Gram-positive bacteria. Folding and maturation of many secreted proteins depend on a single extracellular foldase, the PrsA protein. PrsA
Schmidpeter, Philipp A. M., Hiller, Sebastian, Schmid, Franz X., Hunkeler, Moritz, Burmann, Björn M., Maier, Timm, Jakob, Roman P., Koch, Johanna R., Schmid FX, Schmidpeter, Philip A.M. (author), Jakob RP, Burmann, Björn M. (author), Hunkeler (author), Maier T, Hunkeler M, Hiller S, Burmann BM, Koch, Johanna R. (author), Jakob, Roman (author), Schmidpeter PA, Koch JR   +20 more
core   +1 more source

The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation

FEBS Letters, EarlyView.
Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge, Vivian Pogenberg, Alexander Baumgart, Bente Siebels, Hartmut Schlüter, Michael Hecht‐Bucher, Aymelt Itzen   +6 more
wiley   +1 more source

Bacterial AsmA Proteins Transfer Glycerophospholipids Between Membranes

Contact
Gram-negative bacteria are surrounded by inner and outer membranes whose glycerophospholipid building blocks are synthesized in the inner membrane and redistributed to the outer membrane by poorly understood mechanisms.
Matthew A. Schilling, Karin M. Reinisch
doaj   +1 more source

L‐aspartate oxidase provides new insights into fumarate reduction in anaerobic darkness in Synechocystis sp. PCC6803

FEBS Letters, EarlyView.
Synechocystis strains deficient in succinate dehydrogenase (SDH) secrete more succinate than the WT under dark anaerobic conditions, supporting that SDH then primarily acts as SDH, not as a fumarate reductase. L‐aspartate oxidase (Laspo) from Synechocystis is functional under anaerobic conditions, reducing fumarate to succinate.
Kateryna Kukil, Jeffrey A. Hawkes, Cecilia Blikstad, Pia Lindberg   +3 more
wiley   +1 more source

Surfactant-free purification of membrane protein complexes from bacteria: application to the staphylococcal penicillin-binding protein complex PBP2/PBP2a. [PDF]

, 2014
Surfactant-mediated removal of proteins from biomembranes invariably results in partial or complete loss of function and disassembly of multi-protein complexes. We determined the capacity of styrene-co-maleic acid (SMA) co-polymer to remove components of
Taylor, Peter W, Paulin, S, Roper, David I, Garcia-Lara, J, Roper, DI, Taylor, PW, Galley, Nicola F., Paulin, Sarah, Rosado, H, Foster, SJ, Taylor, Peter W., Dafforn, TR, Dafforn, Tim, Dafforn, Timothy R, Galley, Nicola F, Jamshad, Mohammed, Rosado, Helena, Foster, Simon J, Garcia-Lara, Jorge, Jamshad, M, Galley, NF, Foster, Simon J., Roper, David I.   +22 more
core   +1 more source

Hijacking Complement Regulatory Proteins for Bacterial Immune Evasion

Frontiers in Microbiology, 2016
The human complement system plays an important role in the defense against invading pathogens, inflammation and homeostasis. Invading microbes, such as bacteria, directly activate the complement system resulting in the formation of chemoattractants and ...
Elise Sofie Hovingh, Elise Sofie Hovingh, Bryan van den Broek, Ilse Jongerius, Ilse Jongerius   +4 more
doaj   +1 more source

Artificial molecular machines and motors—Design and control of nanoscale motion

FEBS Letters, EarlyView.
Molecules are constantly moving because of thermal fluctuations, but random motion alone cannot be exploited to perform directional tasks. Artificial molecular machines use chemical, electrical, or light energy to bias this motion. Molecular shuttles, rotary motors, and supramolecular pumps illustrate how nanoscale movement can be controlled and ...
Leonardo Andreoni, Alberto Credi
wiley   +1 more source

Automatic selection of representative proteins for bacterial phylogeny

BMC Evolutionary Biology, 2005
Background Although there are now about 200 complete bacterial genomes in GenBank, deep bacterial phylogeny remains a difficult problem, due to confounding horizontal gene transfers and other phylogenetic "noise".
Goldberg David, Bern Marshall
doaj   +1 more source