Results 81 to 90 of about 1,362,244 (305)

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

FEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon, Angéla Békesi, Latifa Kazzazy, Jázmin Mihály, Máté Varga, Beáta G. Vértessy, Kinga Nyíri   +6 more
wiley   +1 more source

Comparative genome analysis of three classical E. coli cloning strains designed for blue/white selection: JM83, JM109 and XL1‐Blue

FEBS Open Bio
The development of the Escherichia coli K‐12 laboratory strains JM83, JM109 and XL1‐Blue was instrumental in early gene technology. We report the comprehensive genome sequence analysis of JM83 and XL1‐Blue using Illumina and Oxford Nanopore technologies ...
Stefan Achatz, Arne Skerra
doaj   +1 more source

“Super Sandwich” Assay Using Phenylboronic Acid for the Detection of E. coli Contamination: Methods for Application

Microorganisms
This paper proposes a method for E. coli detection in a microplate format using low-molecular-weight compounds that specifically interact with the lipopolysaccharides (LPSs) of E. coli cell walls. These compounds can amplify analytical signals by binding
Anna N. Berlina, Svetlana I. Kasatkina, Margarita O. Shleeva, Anatoly V. Zherdev, Boris B. Dzantiev   +4 more
doaj   +1 more source

Multiple Mechanisms of Enterobacter asburiae strain RS83 for Plant Growth Enhancement [PDF]

Songklanakarin Journal of Science and Technology (SJST), 2015
Enterobacter asburiae strain RS83 having the capacity of promoting plant growth and yield of various vegetable crops was isolated from the rhizosphere of cassava in Phitsanulok province. The objective of this study was to investigate possible bacterial
Kanchalee Jetiyanon
doaj  

Draft Genome Sequence of a Chitinase-producing Biocontrol Bacterium Serratia sp. C-1

Research in Plant Disease, 2015
The chitinase-producing bacterial strain C-1 is one of the key chitinase-producing biocontrol agents used for effective bioformulations for biological control. These bioformulations are mixed cultures of various chitinolytic bacteria.
Seur Kee Park, Young Cheol Kim
doaj   +1 more source

A novel programmable lysozyme-based lysis system in Pseudomonas putida for biopolymer production [PDF]

, 2017
Indexación: Scopus; Web of Science.Cell lysis is crucial for the microbial production of industrial fatty acids, proteins, biofuels, and biopolymers. In this work, we developed a novel programmable lysis system based on the heterologous expression of ...
Borrero de Acuña, J.M., Cabrera, A., Hidalgo-Dumont, C., Pacheco, N., Poblete-Castro, I.   +4 more
core   +1 more source

Alcohol‐induced altered glycans in human tracheal epithelial cells promote bacterial adhesion

FEBS Open Bio, EarlyView.
Alcohol induces altered glycans to promote bacteria adhesion. Heavy alcohol drinking is known to increase the risk of bacterial pneumonia. However, the link between alcohol levels and risk of infection remains underexplored. Recently, we found that alcohol induced α2‐6sialo mucin O‐glycans in human tracheobronchial epithelial cells, which mediated the ...
Pi‐Wan Cheng, Souvik Datta, Derrick R. Samuelson   +2 more
wiley   +1 more source

Production of Extracellular Protease from Bacterial Co-cultures using Solid State Fermentation

Bioscientific Review, 2020
Proteases (also known as peptidases or proteinases) are hydrolytic enzymes that cleave proteins into amino acids. They comprise 60% of the total industrial usage of enzymes worldwide and can be obtained from many sources.
Nabiha Naeem Sheikhs, Qurat-ul-ain, Saba Altaf   +2 more
doaj   +1 more source

Gac two-component system in Pseudomonas syringae pv. tabaci is required for virulence but not for hypersensitive reaction [PDF]

, 2008
Pseudomonas syringae pv. tabaci 6605 causes wildfire disease on host tobacco plants. To investigate the regulatory mechanism of the expression of virulence, Gac two-Component system-defective mutants, Delta gacA and Delta gacS, and a double mutant, Delta
A Bultreys, A Chatterjee, A Schaefer, A Stintzi, B Quinones, B Quinones, C Fuqua, DK Willis, EO King, F Taguchi, F Taguchi, Fumiko Taguchi, J Yu, JW Costerton, Kazuhiro Toyoda, KH McClean, LM Keith, M Castaneda, M Elasri, M Marutani, MD Parkins, Md. Mijan Hossain, MF Alexeyev, Mizuri Marutani, NT Keen, PD Shaw, S Heeb, SL Lopez-Garcia, SM Kirov, ST Chancey, T Kitten, TG Kinscherf, TM Barta, Tomonori Shiraishi, WF Fett, Y Ishiga, Yoshishige Inagaki, Yujiro Ogawa, Yuki Ichinose   +38 more
core   +1 more source

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source