Biofilm producing Salmonella typhi: Chronic colonization and development of gallbladder cancer [PDF]
, 2017 Salmonella enterica subspecies enterica serovar Typhi is the aetiological agent of typhoid or enteric fever. In a subset of individuals, S. Typhi colonizes the gallbladder causing an asymptomatic chronic infection.
Cavallo, Ilaria +4 more
core +1 more source
FEBS Letters, EarlyView.Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho +3 more
wiley +1 more source
In situ molecular organization and heterogeneity of the Legionella Dot/Icm T4SS
FEBS Letters, EarlyView.We present a nearly complete in situ model of the Legionella Dot/Icm type IV secretion system, revealing its central secretion channel and identifying new components. Using cryo‐electron tomography with AI‐based modeling, our work highlights the structure, variability, and mechanism of this complex nanomachine, advancing understanding of bacterial ...
Przemysław Dutka +11 more
wiley +1 more source
Markerless gene deletion in Ralstonia solanacearum based on its natural transformation competence
Frontiers in Microbiology, 2022 Ralstonia solanacearum species complex (RSSC) is a group of Gram-negative bacterial pathogen capable of infecting numerous plants and crops, causing severe vascular wilt diseases.
Jinli Yan +15 more
doaj +1 more source
Force-extension curves of bacterial flagella
, 2010 Bacterial flagella assume different helical shapes during the tumbling phase of a bacterium but also in response to varying environmental conditions. Force-extension measurements by Darnton and Berg explicitly demonstrate a transformation from the coiled
Stark, Holger, Vogel, Reinhard
core +1 more source
Transfer of antibiotic resistance in Staphylococcus aureus [PDF]
, 2017 Staphylococcus aureus is a serious human pathogen with remarkable adaptive powers. Antibiotic-resistant clones rapidly emerge mainly by acquisition of antibiotic-resistance genes from other S. aureus strains or even from other genera.
Haaber, Jakob +2 more
core +1 more source
Cell wall target fragment discovery using a low‐cost, minimal fragment library
FEBS Letters, EarlyView.LoCoFrag100 is a fragment library made up of 100 different compounds. Similarity between the fragments is minimized and 10 different fragments are mixed into a single cocktail, which is soaked to protein crystals. These crystals are analysed by X‐ray crystallography, revealing the binding modes of the bound fragment ligands.
Kaizhou Yan +5 more
wiley +1 more source
A Fast and Practical Yeast Transformation Method Mediated by Escherichia coli Based on a Trans-Kingdom Conjugal Transfer System: Just Mix Two Cultures and Wait One Hour. [PDF]
PLoS ONE, 2016 Trans-kingdom conjugation is a phenomenon by which DNA is transferred into a eukaryotic cell by a bacterial conjugal transfer system. Improvement in this method to facilitate the rapid co-cultivation of donor bacterial and recipient eukaryotic cell ...
Kazuki Moriguchi +3 more
doaj +1 more source
FEBS Letters, EarlyView.We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee +3 more
wiley +1 more source
Characterization of the role that bacterial surface polysaccharide poly N-acetyl glucosamine plays in nonvaccine serotypes of streptococcus pneumoniae colonization and pathogenicity [PDF]
, 2016 Poly N-acetyl glucosamine is a cell surface polysaccharide that has been characterized in Staphylococcus epidermidis and Staphylococcus aureus as involved in biofilm formation and implicated in virulence.
Jiang, Fanny
core +1 more source