Results 71 to 80 of about 470,585 (295)

A binary vector for transferring genomic libraries to plants [PDF]

, 1986
The transformation of mutant plants with a complete recombinant library derived from wild-type DNA followed by assay of transformed plants for complementation of the mutant phenotype is a promising method for the isolation of plant genes.
Simoens, Chris, Alliotte, Thierry, Mendel, R, Müller, A, Schiemann, J, Van Lijsebettens, Maria, Schell, Jeff, Van Montagu, Marc, Inzé, Dirk   +8 more
core   +1 more source

A methionine‐lined active site governs carbocation stabilization and product specificity in a bacterial terpene synthase

FEBS Letters, EarlyView.
This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel, Carl P. O. Helmer, Shani Zev, Ronja Driller, Emily Buhr, Markus Reinbold, Renana Schwartz, Gabriel Foley, Mikael Boden, Daniel Garbe, Gerhard Schenk, Dan Thomas Major, Bernhard Loll, Thomas Brück   +13 more
wiley   +1 more source

AAA+ protein unfoldases—the Moirai of the proteome

FEBS Letters, EarlyView.
AAA+ unfoldases are essential molecular motors that power protein degradation and disaggregation. This review integrates recent cryo‐electron microscopy (cryo‐EM) structures and single‐molecule biophysical data to reconcile competing models of substrate translocation.
Stavros Azinas, Marta Carroni
wiley   +1 more source

Aerobic bacterial transformation and biodegradation of dioxins: a review

Bioresources and Bioprocessing, 2020
Waste generation tends to surge in quantum as the population and living conditions grow. A group of structurally related chemicals of dibenzofurans and dibenzo-p-dioxins including their chlorinated congeners collectively known as dioxins are among the ...
Salametu Saibu, Sunday A. Adebusoye, Ganiyu O. Oyetibo   +2 more
doaj   +1 more source

Development of an efficient transformation system for Dothistromin pini : a thesis presented in partial fulfilment of the requirements for the degree of Masters of Science in Molecular Genetics at Massey University [PDF]

, 1996
A transformation system has been developed for the plant pathogenic fungus Dothistroma pini using a positive selection system based on the Escherichia coli hygromycin B phosphotransferase gene (hph).
Bidlake, Anita Therese
core  

Degradation of lignin β-aryl ether units in Arabidopsis thaliana expressing LigD, LigF and LigG from Sphingomonas paucimobilis SYK-6 [PDF]

, 2017
Lignin is a major polymer in the secondary plant cell wall and composed of hydrophobic interlinked hydroxyphenylpropanoid units. The presence of lignin hampers conversion of plant biomass into biofuels; plants with modified lignin are therefore being ...
Bent, Board, Boerjan, Bonawitz, Bonawitz, Borkhardt, Buanafina, Davin, Dima, Dixon, Dixon, El Amrani, Felipe, Foyer, Gall, Gall, Geu-Flores, Geu-Flores, Goodin, Hause, Herbers, Huang, Kirk, Lallement, Lallement, Lazo, Li, Liu, Liu, Lu, Lu, Mansfield, Masai, Masai, Matsuda, Meux, Morreel, Morreel, Niculaes, Otter, Pauly, Rahimi, Ralph, Ralph, Rogers, Samalova, Sato, Sederoff, Shen, Sonoki, Strack, Tsuji, Van Acker, Vanholme, Vanholme, Vanholme, Vanholme, Vogel, Welinder, Weng, Øbro, Øbro   +61 more
core   +1 more source

TRAIL‐PEG‐Apt‐PLGA nanosystem as an aptamer‐targeted drug delivery system potential for triple‐negative breast cancer therapy using in vivo mouse model

Molecular Oncology, EarlyView.
Aptamers are used both therapeutically and as targeting agents in cancer treatment. We developed an aptamer‐targeted PLGA–TRAIL nanosystem that exhibited superior therapeutic efficacy in NOD/SCID breast cancer models. This nanosystem represents a novel biotechnological drug candidate for suppressing resistance development in breast cancer.
Gulen Melike Demirbolat, Aslihan Kucuk, Omer Erdogan, Samed Ozer, Bensu Kozan, Tugba Cuceli, Erkan Gumus, Evrim Cevik, Ozge Cevik   +8 more
wiley   +1 more source

Bacterial transformation v2 [PDF]

, 2019
The following protocol is for bacterial transformation. To see the full abstract and additional resources, visit the Addgene protocol page.
openaire   +1 more source

Tumour–host interactions in Drosophila: mechanisms in the tumour micro‐ and macroenvironment

Molecular Oncology, EarlyView.
This review examines how tumour–host crosstalk takes place at multiple levels of biological organisation, from local cell competition and immune crosstalk to organism‐wide metabolic and physiological collapse. Here, we integrate findings from Drosophila melanogaster studies that reveal conserved mechanisms through which tumours hijack host systems to ...
José Teles‐Reis, Tor Erik Rusten
wiley   +1 more source

OS EXPERIMENTOS DE GRIFFITH NO ENSINO DE BIOLOGIA: A TRANSPOSIÇÃO DIDÁTICA DO CONCEITO DE TRANSFORMAÇÃO NOS LIVROS DIDÁTICOS

Ensaio Pesquisa em Educação em Ciências, 2010
Griffith's experiments regarding bacterial transformation Pneumococcus are regarded as essential when related to DNA/heredity recognition process. The current study goal is to assess the didactic transposition of the concept of bacterial transformation ...
Caroline Belotto Batisteti, Elaine Sandra Nicolini Nabuco de Araújo, João José Caluzi   +2 more
doaj