Reduced mutation rate and increased transformability of transposon-free Acinetobacter baylyi ADP1-ISx [PDF]
, 2017 The genomes of most bacteria contain mobile DNA elements that can contribute to undesirable genetic instability in engineered cells. In particular, transposable insertion sequence (IS) elements can rapidly inactivate genes that are important for a ...
Barrick, Jeffrey E +3 more
core +2 more sources
Biofilm producing Salmonella typhi: Chronic colonization and development of gallbladder cancer [PDF]
, 2017 Salmonella enterica subspecies enterica serovar Typhi is the aetiological agent of typhoid or enteric fever. In a subset of individuals, S. Typhi colonizes the gallbladder causing an asymptomatic chronic infection.
Cavallo, Ilaria +4 more
core +1 more source
Le mécanisme de la fermentation malolactique des vins
OENO One, 1977 Dans ce travail on étudie l'activité de suspensions bactériennes responsables de la fermentation malolactique des vins, on décrit les méthodes de purification d'extraits bactériens catalysant cette transformation.
M. Lonvaud +2 more
doaj +1 more source
Acquisition of an Agrobacterium Ri Plasmid and Pathogenicity by Other -Proteobacteria in Cucumber and Tomato Crops Affected by Root Mat [PDF]
, 2004 Root mat of cucumbers and tomatoes has previously been shown to be caused by Agrobacterium radiobacter strains harboring a root-inducing Ri plasmid (pRi).
Stead, D.E., Weller, S.A., Young, J.P.W.
core +1 more source
Markerless gene deletion in Ralstonia solanacearum based on its natural transformation competence
Frontiers in Microbiology, 2022 Ralstonia solanacearum species complex (RSSC) is a group of Gram-negative bacterial pathogen capable of infecting numerous plants and crops, causing severe vascular wilt diseases.
Jinli Yan +15 more
doaj +1 more source
A Fast and Practical Yeast Transformation Method Mediated by Escherichia coli Based on a Trans-Kingdom Conjugal Transfer System: Just Mix Two Cultures and Wait One Hour. [PDF]
PLoS ONE, 2016 Trans-kingdom conjugation is a phenomenon by which DNA is transferred into a eukaryotic cell by a bacterial conjugal transfer system. Improvement in this method to facilitate the rapid co-cultivation of donor bacterial and recipient eukaryotic cell ...
Kazuki Moriguchi +3 more
doaj +1 more source
FEBS Letters, EarlyView.Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho +3 more
wiley +1 more source
Transfer of antibiotic resistance in Staphylococcus aureus [PDF]
, 2017 Staphylococcus aureus is a serious human pathogen with remarkable adaptive powers. Antibiotic-resistant clones rapidly emerge mainly by acquisition of antibiotic-resistance genes from other S. aureus strains or even from other genera.
Haaber, Jakob +2 more
core +1 more source
FEBS Letters, EarlyView.In this study, we present the structure of AcrIE8.1, a previously uncharacterized anti‐CRISPR protein that inhibits the type I‐E CRISPR‐Cas system. Through a combination of structural and biochemical analyses, we demonstrate that AcrIE8.1 directly binds to the Cas11 subunit of the Cascade complex to inhibit the CRISPR‐Cas system.
Young Woo Kang, Hyun Ho Park
wiley +1 more source
Native Plasmid-Encoded Mercury Resistance Genes Are Functional and Demonstrate Natural Transformation in Environmental Bacterial Isolates. [PDF]
, 2019 Plasmid-mediated horizontal gene transfer (HGT) is a major driver of genetic diversity in bacteria. We experimentally validated the function of a putative mercury resistance operon present on an abundant 8-kbp native plasmid found in groundwater samples ...
Carlson, Hans K +5 more
core +1 more source