Bacteriophage infection of Escherichia coli leads to the formation of membrane vesicles via both explosive cell lysis and membrane blebbing [PDF]
Membrane vesicles (MVs) are membrane-bound spherical nanostructures that prevail in all three domains of life. In Gram-negative bacteria, MVs are thought to be produced through blebbing of the outer membrane and are often referred to as outer membrane ...
Petty, Nicola K +18 more
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Single-Cell Approach Reveals Intercellular Heterogeneity in Phage-Producing Capacities
Bacteriophage burst size is the average number of phage virions released from infected bacterial cells, and its magnitude depends on the duration of an intracellular progeny accumulation phase.
Sherin Kannoly +6 more
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Structure and Function of the T4 Spackle Protein Gp61.3
The bacteriophage T4 genome contains two genes that code for proteins with lysozyme activity—e and 5. Gene e encodes the well-known T4 lysozyme (commonly called T4L) that functions to break the peptidoglycan layer late in the infection cycle, which is ...
Shuji Kanamaru +5 more
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Intergroup Lysis and Transduction by Streptococcal Bacteriophages [PDF]
Bacteriophages from streptococci of groups A, E, and G lysed streptococci distributed among serogroups A, C, G, H, and L; propagated in some, but not all, of these; and transduced streptomycin resistance within group A or (by A phages only) from group A to group G streptococci.
A E, Colón, R M, Cole, C G, Leonard
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Label-free smartphone quantitation of bacteria by darkfield imaging of light scattering in fluoropolymer micro capillary film allows portable detection of bacteriophage lysis [PDF]
Conventional methods for the detection and quantitation of bacteria are slow, laborious and require a laboratory. Microfluidic systems offer faster and portable testing, and smartphone cameras can record colorimetric or fluorometric bioassays, but this ...
Osborn, Helen M. I. +5 more
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Optimum Threshold Minimizes Noise in Timing of Intracellular Events
Summary: How the noisy expression of regulatory proteins affects timing of intracellular events is an intriguing fundamental problem that influences diverse cellular processes.
Sherin Kannoly +5 more
doaj +1 more source
THE GROWTH OF BACTERIOPHAGE AND LYSIS OF THE HOST [PDF]
1. A new strain of B. coli and of phage active against it is described, and the relation between phage growth and lysis has been studied. It has been found that the phage can lyse these bacteria in two distinct ways, which have been designated lysis from within and lysis from without. 2.
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Genomewide Patterns of Substitution in Adaptively Evolving Populations of the RNA Bacteriophage MS2 [PDF]
Experimental evolution of bacteriophage provides a powerful means of studying the genetics of adaptation, as every substitution contributing to adaptation can be identified and characterized.
Charlesworth, Brian +1 more
core +1 more source
Bacteriophage Therapy as an Application for Bacterial Infection in China
Antibiotic resistance has emerged as a significant issue to be resolved around the world. Bacteriophage (phage), in contrast to antibiotics, can only kill the target bacteria with no adverse effect on the normal bacterial flora.
Shuang Liang +8 more
doaj +1 more source
Factors in Lysis and Lysis Inhibition by Lambda Bacteriophage [PDF]
Groman, Neal B. (University of Washington, Seattle). Factors in lysis and lysis inhibition by lambda bacteriophage. J. Bacteriol. 90: 1563–1568. 1965.—Induced Escherichia coli strain K-12(λ112) exhibited lysis inhibition at 37 C but lysed at 44 C when incubated in LB medium ...
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