Results 1 to 10 of about 169,837 (200)
Purification of Bacteriophage Lambda Repressor [PDF]
Protein Expression and Purification, 2013 Bacteriophage lambda repressor controls the lysogeny/lytic growth switch after infection of E. coli by lambda phage. In order to study in detail the looping of DNA mediated by the protein, tag-free repressor and a loss-of-cooperativity mutant were expressed in E.coli and purified by (1) ammonium sulfate fractionation, (2) anion-exchange chromatography ...
Keith E. Shearwin +2 more
exaly +6 more sources
Revisiting bistability in the lysis/lysogeny circuit of bacteriophage lambda. [PDF]
PLoS ONE, 2014 The lysis/lysogeny switch of bacteriophage lambda serves as a paradigm for binary cell fate decision, long-term maintenance of cellular state and stimulus-triggered switching between states. In the literature, the system is often referred to as "bistable.
Michael Bednarz +3 more
doaj +2 more sources
Bacteriophage Lambda: a Paradigm Revisited [PDF]
Journal of Virology, 2010 ABSTRACT Bacteriophage lambda has an archetypal immunity system, which prevents the superinfection of its Escherichia coli lysogens. It is now known that superinfection can occur with toxigenic lambda-like phages at a high frequency, and here we demonstrate that the superinfection of a lambda lysogen can ...
Paul C M, Fogg +3 more
openaire +3 more sources
On the Internal Structure of Bacteriophage Lambda [PDF]
Journal of General Physiology, 1966 The structure of bacteriophage lambda has been studied by electron microscopy of negatively stained particles. The phage particles will eject their DNA if they are heated or dialyzed against a chelating agent. The ghost particles, so formed, have a channel running down their tails.
exaly +3 more sources
eLife, 2023 Nucleotide and force-dependent mechanisms control how the viral genome of lambda bacteriophage is inserted into capsids.
Bálint Kiss, Miklós Kellermayer
doaj +1 more source
Transduction of bacteriophage lambda by bacteriophage T1 [PDF]
Journal of Virology, 1979 When bacteriophage T1 was grown on bacteriophage lambda-lysogenic cells, phenotypically mixed particles were formed which had the serum sensitivity, host range, and density of T1 but which gave rise to lambda phage. T1 packaged lambda genomes more efficiently both when the length of the prophage was less than that of wild-type lambda and when the host ...
H, Drexler, J R, Christensen
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A one-step miniprep for the isolation of plasmid DNA and lambda phage particles. [PDF]
PLoS ONE, 2011 Plasmid DNA minipreps are fundamental techniques in molecular biology. Current plasmid DNA minipreps use alkali and the anionic detergent SDS in a three-solution format.
George Lezin +4 more
doaj +1 more source
A forward-genetic screen and dynamic analysis of lambda phage host-dependencies reveals an extensive interaction network and a new anti-viral strategy. [PDF]
PLoS Genetics, 2010 Latently infecting viruses are an important class of virus that plays a key role in viral evolution and human health. Here we report a genome-scale forward-genetics screen for host-dependencies of the latently-infecting bacteriophage lambda.
Nathaniel D Maynard +5 more
doaj +1 more source
Bacteriophage lambda as a cloning vector [PDF]
Microbiological Reviews, 1992 Extensive research has been directed toward the development of multipurpose lambda vectors for cloning ever since the potential of using coliphage lambda as a cloning vector was recognized in the late 1970s. An understanding of the intrinsic molecular organization and of the genetic events which determine lysis or lysogeny in lambda has allowed ...
V M, Chauthaiwale +2 more
openaire +2 more sources
Host-parasite coevolution promotes innovation through deformations in fitness landscapes
eLife, 2022 During the struggle for survival, populations occasionally evolve new functions that give them access to untapped ecological opportunities. Theory suggests that coevolution between species can promote the evolution of such innovations by deforming ...
Animesh Gupta +8 more
doaj +1 more source