Results 71 to 80 of about 23,818 (254)

Tn‐seq of Thermus thermophilus Genome Reveals Unexpected Tolerance to Insertions in Bacterial Common Essential Genes

MicrobiologyOpen, Volume 15, Issue 2, April 2026.
Tn‐seq analysis of Thermus thermophilus genome reveals insertions in almost all genes, this is, there is no clear discrimination of essential genes. A similar result has not been reported in other Tn‐seq analysis of bacterial genomes so far. The polyploidy of the Thermus genome could explain the apparent non‐essentiality of key genes.
Cristina L. Gómez‐Campo, Marc Gost, Bruna Fernanda Silva de Sousa, Laura Álvarez, José Berenguer, Modesto Redrejo‐Rodríguez, Mario Mencía   +6 more
wiley   +1 more source

Nanobody‐Mediated c‐MYC Degradation Inhibits Tumor Cell Progression

MedComm, Volume 7, Issue 4, April 2026.
A cell‐permeable nanobody strategy was developed to target the intrinsically disordered oncoprotein c‐MYC. A synthetic nanobody, M4, binds the central PEST region of c‐MYC (241–263) and promotes c‐MYC degradation by enhancing Thr58 phosphorylation. Conjugation of M4 to a cell‐penetrating peptide (CPP) generates CPM4, which efficiently accumulates in ...
Yuanyuan Xue, Hao Jiang, Zhaoyun Zong, Xiaolin Tian, Zelong Miao, Ting Li, Yali Wei, Haiteng Deng   +7 more
wiley   +1 more source

Construction, Characterization, and Application of a Nonpathogenic Virus-like Model for SARS-CoV-2 Nucleocapsid Protein by Phage Display

Toxins, 2022
With the outbreak and spread of COVID-19, a deep investigation of SARS-CoV-2 is urgent. Direct usage of this virus for scientific research could provide reliable results and authenticity.
Yuting Wu, Bing Liu, Zhiwei Liu, Pengjie Zhang, Xihui Mu, Zhaoyang Tong   +5 more
doaj   +1 more source

Improved single-swab sample preparation for recovering bacterial and phage DNA from human skin and wound microbiomes. [PDF]

, 2019
BackgroundCharacterization of the skin and wound microbiome is of high biomedical interest, but is hampered by the low biomass of typical samples. While sample preparation from other microbiomes (e.g., gut) has been the subject of extensive optimization,
Chen, Irene A, Deacon, John M, Kim, Colin Y, Verbanic, Samuel   +3 more
core   +1 more source

Non‐Viral Cytokine‐Inducible SH2 Containing Protein Locus‐Specific Integrated Fibroblast Activation Protein Alpha‐Targeting Chimeric Antigen Receptor T Cells Achieve Potent Antitumor Efficacy in Glioblastoma

MedComm, Volume 7, Issue 4, April 2026.
FAPα is a dual target on glioblastoma cells and perivascular cells. Using a high‐affinity anti‐FAPα nanobody, we generated CISH‐KO FAPα‐CAR‐T cells via non‐viral CRISPR–Cas9 integration. These cells demonstrated enhanced proliferation and superior antitumor efficacy in patient‐derived organoids and orthotopic mouse models compared to conventional CAR‐T
Xin Dong, Yao Sun, Yuetong Guo, Jiao Wang, Fei Wang, Ziming Wang, Ruizhen Li, Fei Xie, Tingting Tan, Baijie Cheng, Ronghan Huang, Shu Zhang, Xiaotong Lin, Zhaoze Guo, Hubing Wu, Hao Wu, Xubiao Zhang, Guozhu Xie   +17 more
wiley   +1 more source

Novel applications of shotgun phage display [PDF]

, 2004
In a shotgun phage display library, theoretically, the entire proteome of a bacterium is represented. Phages displaying specific polypeptides can be isolated by affinity selection, while the corresponding gene remains physically linked to the gene ...
Rosander, Anna
core  

Molecular models and structural comparisons of native and mutant class I filamentous bacteriophages Ff (fd, f1, M13), If1 and IKe [PDF]

, 1994
The filamentous bacteriophages are flexible rods about 1 to 2 microns long and 6 nm in diameter, with a helical shell of protein subunits surrounding a DNA core. The approximately 50-residue coat protein subunit is largely alpha-helix and the axis of the
Hale, RD, Helmer-Citterich, M, Marvin, DA, Nave, C   +3 more
core   +2 more sources

Inhibition of Targeted Bacterial Growth Using MazF‐Expressing Phagemids: A Novel Therapeutic Approach Based on Endoribonuclease Toxin Molecules

Microbiology and Immunology, Volume 70, Issue 4, Page 206-213, April 2026.
ABSTRACT The extensive and indiscriminate use of antibiotics, which are intended to halt bacterial growth or eliminate them, has unintentionally given rise to antimicrobial resistance (AMR), which is a critical threat to global health. In the pursuit of alternative solutions, researchers have focused on type II toxin–antitoxin systems prevalent within ...
Hana Hasegawa, Tatsuki Miyamoto, Rino Isshiki, Tomoyoshi Kaneko, Kenichi Takasugi, Naohiro Noda, Satoshi Tsuneda   +6 more
wiley   +1 more source

Development of a high-throughput method to screen novel antiviral materials.

PLoS ONE, 2022
Respiratory infectious diseases pose a serious threat worldwide, and novel antiviral materials are highly demanded. Photocatalytic nanoparticles have been developed to inhibit indirect transmission of pathogens by acting as surface coating materials ...
Makoto Nakakido, Naoki Tanaka, Ayako Shimojo, Nobuhiro Miyamae, Kouhei Tsumoto   +4 more
doaj   +1 more source

Conjugative transfer of ICESde3396 between three β-hemolytic streptococcal species [PDF]

, 2014
Background: Integrative conjugative elements (ICEs) are mobile genetic elements (MGEs) that possess all genes necessary for excision, transfer and integration into recipient genome. They also carry accessory genes that impart new phenotypic features to
Bauer, Michelle J., Cameron, Ainslie, McMillan, David J., McNeilly, Celia L., Shera, Josephine, Smyth, Danielle J., Sriprakash, Kadaba S.   +6 more
core   +1 more source