Results 101 to 110 of about 73,636 (212)
PLoS ONE, 2014 BackgroundBoth tumor cells and their supporting endothelial cells should be considered for targeted cell killing when designing cancer treatments.
Min Zhang +5 more
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BMC Biotechnology, 2018 Background The Baculovirus expression vector system (BEVS) is a transient expression platform for recombinant protein production in insect cells. Baculovirus infection of insect cells will shutoff host translation and induce apoptosis and lead to the ...
Xiaoyue Zhang +4 more
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HFE and transferrin directly compete for transferrin receptor in solution and at the cell surface [PDF]
, 2004 Transferrin receptor (TfR) is a dimeric cell surface protein that binds both the serum iron transport protein transferrin (Fe-Tf) and HFE, the protein mutated in patients with the iron overload disorder hereditary hemochromatosis.
Bjorkman, Pamela J. +1 more
core +2 more sources
Directional and direct cloning strategy for high-throughput generation of recombinant baculoviruses
BioTechniques, 2006 The baculovirus expression vector system (BEVS) has become one of the most widely used systems for routine protein expression. We have developed an improved strategy to clone foreign genes directionally and directly into the baculovirus genome vector via
Qi Ma, Li Zhou, Lixin Ma, Keke Huo
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Engineering a serum-resistant and thermostable vesicular stomatitis virus G glycoprotein for pseudotyping retroviral and lentiviral vectors. [PDF]
, 2013 Vesicular stomatitis virus G glycoprotein (VSV-G) is the most widely used envelope protein for retroviral and lentiviral vector pseudotyping; however, serum inactivation of VSV-G pseudotyped vectors is a significant challenge for in vivo gene delivery ...
Hwang, B-Y, Schaffer, DV
core
SIC1 is ubiquitinated in vitro by a pathway that requires CDC4, CDC34, and cyclin/CDK activities [PDF]
, 1997 Traversal from G1 to S-phase in cycling cells of budding yeast is dependent on the destruction of the S-phase cyclin/CDK inhibitor SIC1. Genetic data suggest that SIC1 proteolysis is mediated by the ubiquitin pathway and requires the action of CDC34 ...
Deshaies, Raymond J. +2 more
core
Cell cycle regulation of a Xenopus Wee1-like kinase [PDF]
, 1995 Using a polymerase chain reaction-based strategy, we have isolated a gene encoding a Wee1-like kinase from Xenopus eggs. The recombinant Xenopus Wee1 protein efficiently phosphorylates Cdc2 exclusively on Tyr- 15 in a cyclin-dependent manner.
Coleman, Thomas R. +2 more
core
Expression of baculovirus P35 prevents cell death in Drosophila [PDF]
, 1994 The baculovirus P35 protein functions to prevent apoptotic death of infected cells. We have expressed P35 in the developing embryo and eye of the fly Drosophila melanogaster. P35 eliminates most, if not all, normally occurring cell death in these tissues,
Hay, Bruce A. +2 more
core
Frontiers in MedicineIntroductionRecombinant adeno-associated virus (rAAV) vectors provide a safe and efficient means for in vivo gene delivery, although its large-scale production remains challenging. Featuring high manufacturing speed, flexible product design, and inherent
Ningguang Luo +6 more
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A viral expression factor behaves as a prion
Nature Communications, 2019 Prion-forming proteins have been found in animals, plants, fungi, and bacteria. Here, Nan et al. report that a baculovirus-encoded protein behaves as prion in a yeast system and forms aggregates at high multiplicity of infection in insect cells that ...
Hao Nan +3 more
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