Results 81 to 90 of about 52,750 (225)
A novel baculovirus-derived promoter with high activity in the baculovirus expression system
PeerJ, 2016 The baculovirus expression vector system (BEVS) has been widely used to produce a large number of recombinant proteins, and is becoming one of the most powerful, robust, and cost-effective systems for the production of eukaryotic proteins.
Martínez-Solís, M. +4 more
openaire +4 more sources
VIEW, EarlyView.Nanobodies, derived from the variable domains of camelid heavy‐chain‐only antibodies, have emerged as transformative biomedical tools due to their nanoscale size, exceptional stability, and unique capacity to recognize cryptic epitopes. This review provides a comprehensive overview of the field, outlining the structural and biochemical features of ...
Zhenrui Ye, Xianyang Li, Meixiao Zhan
wiley +1 more source
Iflaviruses in arthropods: when small is mighty
Insect Science, EarlyView.Many arthropod species harbor iflaviruses, which often cause covert (asymptomatic) infections, but may still affect host fitness. We review the impact of iflaviruses on arthropod fitness, immunity, behaviour as well as the iflavirus’ host range, transmission, tissue tropism and the interactions with other microorganisms within arthropods.
Annamaria Mattia +3 more
wiley +1 more source
Gene expression after baculovirus transduction.
, 2023 (a) EGFP-baculovirus expression in Sf9 cells eluted from hydrogels after one day. The EGFP expression from a PVA-gelatin hydrogel is presented as a fluorescent image of DAPI-stained Sf9 cells (the scale bar represents 0.5 mm).
Ahmed Abosalha (17020426) +7 more
core +1 more source
Transgenic selection and underlying mechanisms in apicomplexan parasites
The FEBS Journal, EarlyView.Transgenic selection markers have driven genetic engineering in apicomplexans, enabling precise, iterative experiments. This review discusses mechanistic details of drug selection markers, strategies for marker recycling, and practical considerations for several clinically relevant parasites.
Swaroop Peddiraju +2 more
wiley +1 more source
Overview of the Baculovirus Expression System
Current Protocols in Protein Science, 2018 AbstractThis unit provides information on the replication cycle of insect baculovirus to provide an understanding of how this virus has been adapted for use as an expression vector for recombinant proteins in insect cells. We provide an overview of the virus structure and its unique bi‐phasic replication cycle, which has been exploited in developing ...
Chambers, A +5 more
openaire +3 more sources
Conserved dimerization architecture in C‐type lectins from virus‐vector mosquitoes
The FEBS Journal, EarlyView.C‐type lectins (CTLs) are involved in the detection of viral/microbial carbohydrates. We studied a family of soluble CTLs in the mosquito Aedes aegypti, which acts as a vector for viruses like dengue and Zika. Using crystallography, biophysics, and structure prediction approaches, we found that these proteins consistently pair together in the same ...
Mattia Bertinelli +6 more
wiley +1 more source
Development of a replication-defective baculovirus platform [PDF]
, 2022 Baculovirus expression vector systems are used for the production of an increasing number of licensed commercial subunit vaccines. Though baculoviruses are incapable of replicating outside a narrow host range of lepidopteran insects, a replication ...
Ackerman, Scott Ryan
core
The FEBS Journal, EarlyView.The C. elegans Shu complex (RFS‐1/RIP‐1/SWS‐1) is a DNA‐dependent ATPase that regulates RAD‐51 filaments during homologous recombination. The trimer preferentially binds 5′‐exposed DNA, remodels RAD‐51 filaments via ATP hydrolysis, and stabilizes filaments through ATP binding.
Sam Siu Hang Chu +4 more
wiley +1 more source
Insect Molecular Biology, EarlyView.Parasitoid wasps deploy maternal and embryonic factors to reprogramme host physiology. Venom, calyx fluid, polydnaviruses, teratocytes and larval secretions act in a coordinated, compartmentalised manner. Host‐regulation factors are promising sources of insecticidal, antimicrobial and bioinspired translational molecules.
Ciro Pedro G. Pinto +2 more
wiley +1 more source