Results 71 to 80 of about 68,162 (268)

Gene expression after baculovirus transduction.

, 2023
(a) EGFP-baculovirus expression in Sf9 cells eluted from hydrogels after one day. The EGFP expression from a PVA-gelatin hydrogel is presented as a fluorescent image of DAPI-stained Sf9 cells (the scale bar represents 0.5 mm).
Ahmed Abosalha (17020426), Jacqueline L. Boyajian (17020420), Rahul Thareja (17020423), Karan Arora (628870), Dominique Shum-Tim (1332057), Satya Prakash (9273), Paromita Islam (17020417), Sabrina Schaly (17020414)   +7 more
core   +1 more source

Respuesta de IFN-ɤ e IL-4 en ratones inoculados con una proteína G recombinante del virus de la rabia

Revista Mexicana de Ciencias Pecuarias, 2015
El objetivo de este estudio fue comparar el comportamiento de El objetivo de este estudio fue comparar el comportamiento de El objetivo de este estudio fue comparar el comportamiento de IFN-ɤ e IL-4 en ratones inoculados con proteína G recombinante del ...
Edith Rojas-Anaya, Fernando Esquivel-Guadarrama, Estela Escribano-Romero, Ana Belén Blázquez, Graciela Tapia Pérez, Lilia Lilia Soto Ruíz, Elizabeth Loza-Rubio   +6 more
doaj   +1 more source

Evaluation of an inducible knockout system in insect cells based on co-infection and CRISPR/Cas9.

PLoS ONE, 2023
Due to comparably high product titers and low production costs, the baculovirus/insect cell expression system is considered a versatile production platform in the biopharmaceutical industry.
Christina Sophie Hausjell, Miriam Klausberger, Wolfgang Ernst, Reingard Grabherr   +3 more
doaj   +1 more source

The Influence of Drosophila Spire and Myosin V During Mid‐Oogenesis Is Independent of Their Direct Interaction

Cytoskeleton, EarlyView.
ABSTRACT Cooperativity between cytoskeletal proteins is crucial for spatiotemporal coordination in biological processes, like oogenesis. In mammalian and Drosophila oogenesis, proper assembly and function of actin networks require coordination between actin assembly factors Spire and formins, as well as actin‐associated proteins like myosins and Rab ...
Joseph Y. Ong, Hannah M. Bailey, Emma Carley, Michelle Panzica, Merin Rixen, Neil Billington, Rong Liu, Margot E. Quinlan   +7 more
wiley   +1 more source

Autographa californica Multicapsid Nucleopolyhedrovirus efficiently infects Sf9 cells and transduces mammalian cells via direct fusion with the plasma membrane at low pH

, 2010
The budded virus (BV) of the Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) infects insect cells and transduces mammalian cells mainly through the endocytosis pathway.
F. Deng, M. Wang, Dong, S., Vlak, J.M., Deng, F., S. Dong, Wang, M., Qiu, Z., Z. Hu, Hu, Z.H., J. M. Vlak, H. Wang, Z. Qiu, Wang, H.L.   +13 more
core   +1 more source

Construction of a Baculovirus vector containing A subunit of Shiga toxin for protein delivery

Iranian Journal of Microbiology, 2013
Background and Objectives: Baculovirus can be used as a vector in gene delivery system. Viral envelope of baculovirus would display expressed protein/peptide and it could render as a potential vaccine delivery system.
Mana Oloomi, Saeid Bouzari, Maryam Imani, Narges Akhtarian   +3 more
doaj  

WSSV ie1 promoter is more efficient than CMV promoter to express H5 hemagglutinin from influenza virus in baculovirus as a chicken vaccine

BMC Microbiology, 2008
Background The worldwide outbreak of influenza A (H5N1) viruses among poultry species and humans highlighted the need to develop efficacious and safe vaccines based on efficient and scaleable production. Results White spot syndrome virus (WSSV) immediate-
Yu Li, Ho YuenFern, He Fang, Kwang Jimmy
doaj   +1 more source

Expression, Purification, and Microscopy‐Based Assays for Engineered Recombinant Tyrosinated, Detyrosinated, and Δ2 Human Tubulin

Cytoskeleton, EarlyView.
ABSTRACT Microtubules are noncovalent polymers assembled from α/β tubulin dimers. Their structure, dynamics and interaction with effectors are regulated through the expression of diverse tubulin isotypes and chemically diverse posttranslational modifications, also known as the “tubulin code.” Understanding the biophysical correlates between tubulin ...
Jiayi Chen, Agnieska Szyk, Antonina Roll‐Mecak   +2 more
wiley   +1 more source

Highly efficient baculovirus-mediated multigene delivery in primary cells

Nature Communications, 2016
Multigene delivery and subsequent cellular expression is emerging as a key technology required in diverse research fields including, synthetic and structural biology, cellular reprogramming and functional pharmaceutical screening.
M. Mansouri, Itxaso Bellón-Echeverría, Aurélien Rizk, Zahra Ehsaei, C. Cosentino, Catarina S. Silva, Ye Xie, F. Boyce, M. Davis, S. Neuhauss, V. Taylor, K. Ballmer-Hofer, I. Berger, I. Berger, Philipp Berger   +14 more
semanticscholar   +1 more source

Partial Functional Rescue of Helicoverpa armigera Single Nucleocapsid Nucleopolyhedrovirus Infectivity by Replacement of F Protein with GP64 from Autographa californica Multicapsid Nucleopolyhedrovirus

, 2010
Two distinct envelope fusion proteins (EFPs) (GP64 and F) have been identified in members of the Baculoviridae family of viruses. F proteins are found in group II nucleopolyhedroviruses (NPVs) of alphabaculoviruses and in beta- and deltabaculoviruses ...
Feifei Yin, Shu Shen, Just M. Vlak, Fei Deng, Shen, S., Vlak, J.M., Deng, F., Manli Wang, Ying Tan, Yin, F.F., Hu, Z.H., Wang, M.L., Hualin Wang, Zhihong Hu, Tan, Y., Wang, H.L.   +15 more
core   +1 more source