Results 51 to 60 of about 52,750 (225)

Evaluation of an inducible knockout system in insect cells based on co-infection and CRISPR/Cas9.

PLoS ONE, 2023
Due to comparably high product titers and low production costs, the baculovirus/insect cell expression system is considered a versatile production platform in the biopharmaceutical industry.
Christina Sophie Hausjell, Miriam Klausberger, Wolfgang Ernst, Reingard Grabherr   +3 more
doaj   +1 more source

TED1 Promotes the Assembly of SCFD3‐D14‐D53 to Reinforce Strigolactone Signaling in Rice

Advanced Science, EarlyView.
This study identifies TED1 as a positive regulator of strigolactones (SL) signaling in rice. The ted1 mutants display dwarfism and increased tillering with significantly reduced SLs sensitivity. Through its longer C‐terminal region, TED1 interacts with D53 and D14 to promote the assembly of SCFD3‐D14‐D53, thereby facilitating the efficient ...
Sheng Luo, Chuanyin Wu, Miao Feng, Wenfan Luo, Rong Miao, Jinsheng Qian, Fan Wang, Chunlei Zhou, Bojuan Liu, Jiale Shao, Ziqi Xun, Yehui Xiong, Zhichao Zhao, Cailin Lei, Shanshan Zhu, Yulong Ren, Xin Wang, Xiuping Guo, Haiyang Wang, Zichao Li, Zhijun Cheng, Qibing Lin, Jianmin Wan   +22 more
wiley   +1 more source

Respuesta de IFN-ɤ e IL-4 en ratones inoculados con una proteína G recombinante del virus de la rabia

Revista Mexicana de Ciencias Pecuarias, 2015
El objetivo de este estudio fue comparar el comportamiento de El objetivo de este estudio fue comparar el comportamiento de El objetivo de este estudio fue comparar el comportamiento de IFN-ɤ e IL-4 en ratones inoculados con proteína G recombinante del ...
Edith Rojas-Anaya, Fernando Esquivel-Guadarrama, Estela Escribano-Romero, Ana Belén Blázquez, Graciela Tapia Pérez, Lilia Lilia Soto Ruíz, Elizabeth Loza-Rubio   +6 more
doaj   +1 more source

Iflavirus increases its infectivity and physical stability in association with baculovirus [PDF]

PeerJ, 2016
Virus transmission and the prevalence of infection depend on multiple factors, including the interaction with other viral pathogens infecting the same host.
Agata K. Jakubowska, Rosa Murillo, Arkaitz Carballo, Trevor Williams, Jan W.M. van Lent, Primitivo Caballero, Salvador Herrero   +6 more
doaj   +2 more sources

RSF1‐Dependent PAR Turnover Promotes 53BP1 Liquid Condensate Formation at DNA Damage Sites

Advanced Science, EarlyView.
At sites of DNA damage, RSF1 recruits PARG to accelerate PAR turnover, triggering a switch from PAR‐driven condensates to 53BP1 condensates. This condensate transition enables p53‐dependent gene transcription and coordinates the DNA damage response.
Yungyeong Heo, Yonghyeon Kim, Nangyeong Hwang, Gwanho Sung, Sujin Jo, Yeonhu Lee, Ho‐Chul Kang, Jae‐Ho Lee, Yong Won Choi, Hyeseong Cho, Sunwoo Min   +10 more
wiley   +1 more source

Construction of a Baculovirus vector containing A subunit of Shiga toxin for protein delivery

Iranian Journal of Microbiology, 2013
Background and Objectives: Baculovirus can be used as a vector in gene delivery system. Viral envelope of baculovirus would display expressed protein/peptide and it could render as a potential vaccine delivery system.
Mana Oloomi, Saeid Bouzari, Maryam Imani, Narges Akhtarian   +3 more
doaj  

Original Article. Topical treatment of LdMNPV-infected gypsy moth caterpillars with 18 nucleotides long antisense fragment from LdMNPV IAP3 gene triggers higher levels of apoptosis in infected cells and mortality of the pest

Journal of Plant Protection Research, 2016
The high efficiency of baculovirus infection is partially explained by the ability of the virus to suppress host defense machinery connected with the apoptosis pathway.
Oberemok Volodymyr V., Laikova Kateryna V., Zaitsev Alexey S., Nyadar Palmah M., Gninenko Yuri I., Gushchin Vladimir A., Makarov Valentin V., Agranovsky Alexey A.   +7 more
doaj   +1 more source

What Makes an “Ideal” Cell Line for Recombinant Adeno‐Associated Virus Production?

Biotechnology and Bioengineering, EarlyView.
Several host cell types have been used to produce rAAVs to date. Cell line‐specific traits that are beneficial in the context of rAAV manufacturing are reviewed here, with the goal of developing a consensus on the ‘ideal’ characteristics that an rAAV production host should possess (created with Biorender.com).
James Conheady, Nicholas Donohue, Alexandra Bogdanovic, Sharon Davin, Brian Glennon, Niall Barron, C. Mark Smales   +6 more
wiley   +1 more source

Baculovirus expression system for magnetic sorting of infected cells and enhanced titer determination

BioTechniques, 2004
Recombinant baculoviruses derived from the Autographa californica nuclear polyhedrosis virus (AcNPV) are widely used to express heterologous genes in insect cells, but the use of the baculovirus expression vector system (BEVS) is hampered by slow and ...
Björn Philipps, Michael Forstner, Lorenz M. Mayr   +2 more
doaj   +1 more source

Purification of Disassembled Capsid Protein by Hydroxyapatite Chromatography Enhances Virus‐Like Particle Recovery

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Virus‐like particles (VLPs) are a promising modality with extensive applications in prophylactic and therapeutic vaccine design. Human Papillomavirus (HPV) vaccines are one such example where VLP vaccine applications have led to the successful reduction in HPV‐associated diseases, such as cervical cancer.
Nicholas Samuelson, Nathaniel Parker, Shiyi Wang, Sara Daley, Aaron Cote, Michael A. Winters   +5 more
wiley   +1 more source