Results 51 to 60 of about 68,162 (268)

Comprehensive Comparison of Baculoviral and Plasmid Gene Delivery in Mammalian Cells

Viruses
(1) Recombinant protein production in mammalian cells is either based on transient transfection processes, often inefficient and underlying high batch-to-batch variability, or on laborious generation of stable cell lines.
Maria Toth, Manuel Reithofer, Gregory Dutra, Patricia Pereira Aguilar, Astrid Dürauer, Reingard Grabherr   +5 more
doaj   +1 more source

Comparative Evaluation of Human Papillomavirus Type 16 L1 Protein Expressed in Plasmid- and Baculovirus-Based Systems in Insect Cells [PDF]

Archives of Razi Institute, 2020
Human papillomavirus (HPV) has been associated with specific types of papillomas, lesions at particular anatomic sites, and malignancies. The HPV16 and HPV18 have been shown to play a role in a variety of carcinomas.
B. Abedi Kiasari
doaj   +1 more source

DNA photolyases of Chrysodeixis chalcites nucleopolyhedrovirus are targeted to the nucleus and interact with chromosomes and mitotic spindle structures

, 2010
Cyclobutane pyrimidine dimer (CPD) photolyases convert UV-induced CPDs in DNA into monomers using visible light as the energy source. Two phr genes encoding class II CPD photolyases PHR1 and PHR2 have been identified in Chrysodeixis chalcites ...
Xu, F. (Fang), van Oers, MM, Oers, M.M. (Monique) van, van Oers, M.M., Vlak, J.M. (Just), Vlak, J.M., Eker, A.P.M., Vlak, JM, Xu, F., Xu, F, Oers, M.M., van, Eker, APM, Eker, A.P.M. (André)   +12 more
core   +1 more source

EXTL3 and NPC1 are mammalian host factors for Autographa californica multiple nucleopolyhedrovirus infection

Nature Communications
Baculovirus is an obligate parasitic virus of the phylum Arthropoda. Baculovirus including Autographa californica multiple nucleopolyhedrovirus (AcMNPV) has been widely used in the laboratory and industrial preparation of proteins or protein complexes ...
Yuege Huang, Hong Mei, Chunchen Deng, Wei Wang, Chao Yuan, Yan Nie, Jia-Da Li, Jia Liu   +7 more
doaj   +1 more source

Recombinant neuraminidase pseudotyped baculovirus: a dual vector for delivery of Angiotensin II peptides and DNA vaccine

AMB Express, 2018
Baculovirus is a promising vaccine deliver vector due to its biosafety profiles, gene transfer efficiency, ability to display small foreign antigens on its surface, strong adjuvant activities, etc.
Irisa Trianti, Saengchai Akeprathumchai, Phenjun Mekvichitsaeng, Sansanalak Rachdawong, Kanokwan Poomputsa   +4 more
doaj   +1 more source

The Complete Genome Sequence of Plodia Interpunctella Granulovirus: Evidence for Horizontal Gene Transfer and Discovery of an Unusual Inhibitor-of-Apoptosis Gene. [PDF]

PLoS ONE, 2016
The Indianmeal moth, Plodia interpunctella (Lepidoptera: Pyralidae), is a common pest of stored goods with a worldwide distribution. The complete genome sequence for a larval pathogen of this moth, the baculovirus Plodia interpunctella granulovirus (PiGV)
Robert L Harrison, Daniel L Rowley, C Joel Funk   +2 more
doaj   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

A new single-step protocol for rapid baculovirus-driven protein production in insect cells

BMC Biotechnology, 2017
BackgroundIn the last three decades, the Baculovirus expression vector system (BEV) has evolved to one of the most widely used eukaryotic systems for heterologous protein expression including approved vaccines and therapies.
Judith D. Scholz, S. Suppmann
semanticscholar   +1 more source

Residual baculovirus in insect cell-derived influenza virus-like particle preparations enhances immunogenicity.

PLoS ONE, 2012
Influenza virus-like particles are currently evaluated in clinical trials as vaccine candidates for influenza viruses. Most commonly they are produced in baculovirus- or mammalian- expression systems.
Irina Margine, Luis Martinez-Gil, Yi-Ying Chou, Florian Krammer   +3 more
doaj   +1 more source

Allosteric Inhibition of Polycomb Repressive Complex 2 by an EZH2‐Selective Small Molecule Inhibitor

Advanced Science, EarlyView.
The study characterizes C36, a highly selective EZH2/PRC2 inhibitor that acts via a novel allosteric mechanism. Unlike previous inhibitors, C36 inhibits EZH2/PRC2 by disrupting the allosteric communication between EZH2 and EED in a SAM‐noncompetitive manner.
Ting Cao, Dongdong Liu, Haishan Gao, Chenyang Qin, Wen Zhang, Ziyun Lu, Dongxia Tan, Yuxiu Qu, Yusong Liu, Zhiran Zou, Hongtao Yu, Wei Qi   +11 more
wiley   +1 more source