Results 51 to 60 of about 39,940 (191)

Genome Scale Transcriptomics of Baculovirus-Insect Interactions

Viruses, 2013
Baculovirus-insect cell technologies are applied in the production of complex proteins, veterinary and human vaccines, gene delivery vectors‚ and biopesticides.
Steven Reid, Lars K. Nielsen, Quan Nguyen   +2 more
doaj   +1 more source

Adenosine Receptor Modulates Permissiveness of Baculovirus (Budded Virus) Infection via Regulation of Energy Metabolism in Bombyx mori

Frontiers in Immunology, 2020
Although the modulation of host physiology has been interpreted as an essential process supporting baculovirus propagation, the requirement of energy supply for host antivirus reactions could not be ruled out.
Yu-Hsien Lin, Yu-Hsien Lin, Chia-Chi Tai, Václav Brož, Cheng-Kang Tang, Ping Chen, Carol P. Wu, Cheng-Hsun Li, Yueh-Lung Wu   +8 more
doaj   +1 more source

Expression, Purification, and Microscopy‐Based Assays for Engineered Recombinant Tyrosinated, Detyrosinated, and Δ2 Human Tubulin

Cytoskeleton, EarlyView.
ABSTRACT Microtubules are noncovalent polymers assembled from α/β tubulin dimers. Their structure, dynamics and interaction with effectors are regulated through the expression of diverse tubulin isotypes and chemically diverse posttranslational modifications, also known as the “tubulin code.” Understanding the biophysical correlates between tubulin ...
Jiayi Chen, Agnieska Szyk, Antonina Roll‐Mecak   +2 more
wiley   +1 more source

Baculovirus expression system for magnetic sorting of infected cells and enhanced titer determination

BioTechniques, 2004
Recombinant baculoviruses derived from the Autographa californica nuclear polyhedrosis virus (AcNPV) are widely used to express heterologous genes in insect cells, but the use of the baculovirus expression vector system (BEVS) is hampered by slow and ...
Björn Philipps, Michael Forstner, Lorenz M. Mayr   +2 more
doaj   +1 more source

Sodium Ions Affect GPR17 Conformational States and Functionality

Proteins: Structure, Function, and Bioinformatics, EarlyView.
ABSTRACT Sodium is an allosteric modulator of several class‐A GPCR regulating active versus inactive conformation state and may be a critical issue for the optimization of crystallization procedures. Herein, the role of Na+ in the regulation of GPR17 conformational states was studied through MD simulations of GPR17 wild‐type and mutated on two ...
Luca Palazzolo, Simona Daniele, Davide Bianchi, Lara Russo, Stefano Capaldi, Maria Letizia Trincavelli, Ivano Eberini   +6 more
wiley   +1 more source

Replication of baculovirus DNA

Journal of General Virology, 1995
Introduction. The Baculoviridae is a diverse family of pathogens that are infectious for arthropods and are characterized by a complex replication cycle that culminates in the occlusion of virions in a crystalline protein matrix (Blissard & Rohrmann, 1990).
Kool, M., Ahrens, C.H., Vlak, J.M., Rohrmann, G.F.   +3 more
openaire   +3 more sources

Active pH Modulation by Proton Channel‐Peptide Nucleic Acid Complex for Effective siRNA Endosomal Escape

Small, EarlyView.
A bioinspired siRNA nanocarrier is engineered by integrating an influenza A M2 proton channel with siRNA through a peptide nucleic acid linker on liposome surfaces. Active proton transport amplifies osmotic stress within endosomes, triggering membrane rupture and cytosolic release.
Seongmin Ga, Nam Hyeong Kim, Si Yeon Ryu, Kyeonghyun Kim, Eun Sung Kang, Hayeon Bae, Seungmin Ryu, Suhyeon Kim, Yeontae Jang, Bok‐Soo Lee, Jiyoung Nam, Minah Suh, Jaecheol Lee, Seung‐gu Kang, Jaekyung Hyun, Huong T. Kratochvil, Yong Ho Kim   +16 more
wiley   +1 more source

In vivo transcriptional targeting into the retinal vasculature using recombinant baculovirus carrying the human flt-1 promoter

Virology Journal, 2007
Background Endothelial cells are a target for gene therapy because they are implicated in a number of vascular diseases. Recombinant baculovirus have emerged as novel gene delivery vectors. However, there is no information available concerning the use of
Vaca Luis, Clapp Carmen, Aranda Jorge, Luz-Madrigal Agustín   +3 more
doaj   +1 more source

Nanobodies as next‐generation targeting platforms: From discovery technologies to translational biomedicine

VIEW, EarlyView.
Nanobodies, derived from the variable domains of camelid heavy‐chain‐only antibodies, have emerged as transformative biomedical tools due to their nanoscale size, exceptional stability, and unique capacity to recognize cryptic epitopes. This review provides a comprehensive overview of the field, outlining the structural and biochemical features of ...
Zhenrui Ye, Xianyang Li, Meixiao Zhan
wiley   +1 more source

Copper Treatment Increases Recombinant Baculovirus Production and Polyhedrin and p10 Expression

BioTechniques, 1997
Treatment with 2 mM CuSO4 was used to induce a Drosophila melanogaster metallothionein (Mtn) promoter that had been cloned into a recombinant baculovirus.
Lorene M. Lanier, Kathleen Storm, Alireza Shafaie, Loy E. Volkman   +3 more
doaj   +1 more source