Results 31 to 40 of about 68,162 (268)
A rapid method for titrating baculovirus stocks using the Sf-9 Easy Titer cell line
A new rapid method for titrating baculovirus stocks has been developed using a novel cell line Sf-9 Easy Titer (Sf-9ET). The Sf-9ET cell line has been transfected with plasmid DNA containing the enhanced green fluorescent protein (eGFP) gene under the ...
Ralph F. Hopkins, Dominic Esposito
doaj +1 more source
Adjuvant-free immunization with hemagglutinin-Fc fusion proteins as an approach to influenza vaccines [PDF]
The hemagglutinins (HAs) of human H1 and H3 influenza viruses and avian H5 influenza virus were produced as recombinant fusion proteins with the human immunoglobulin Fc domain.
Temperton, Nigel J. +24 more
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Baculovirus-mediated gene transfer has been developed as a vaccine design strategy against a number of diseases without apparent viral replication. However, it has been hampered by complement-dependent inactivation, thus hindering the in vivo application
Zehui Liu +6 more
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Baculovirus as a vaccine vector [PDF]
Baculovirus is extensively utilized as an excellent tool for production of recombinant protein in insect cells. Baculovirus infects insects in nature and is non-pathogenic to humans. In addition to insect cells, baculovirus is capable of transducing a broad range of animal cells.
Hsin-Yu, Lu +2 more
openaire +3 more sources
Baculovirus as Versatile Vectors for Protein Display and Biotechnological Applications.
The baculovirus-insect cell system has long been deployed for a variety of applications including for use as biopesticides, for recombinant protein production, transient transgene expression, tissue therapy, and for vaccine production.
Chih-Hsuan Tsai +3 more
semanticscholar +1 more source
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Brady, Marcia
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MultiBac: Baculovirus-Mediated Multigene DNA Cargo Delivery in Insect and Mammalian Cells
The baculovirus/insect cell system (BICS) is widely used in academia and industry to produce eukaryotic proteins for many applications, ranging from structure analysis to drug screening and the provision of protein biologics and therapeutics.
K. Gupta +5 more
semanticscholar +1 more source
Background Insect baculovirus-produced Human immunodeficiency virus type 1 (HIV-1) Gag virus-like-particles (VLPs) stimulate good humoral and cell-mediated immune responses in animals and are thought to be suitable as a vaccine candidate.
Shephard Enid G +5 more
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Recent Developments in the Use of Baculovirus Expression Vectors.
Over 35 years since it was established to make recombinant proteins, the baculovirus expression vector system continues to develop and improve. Early systems for recombinant virus selection were laborious, but better methods were rapidly devised that ...
R. Possee +4 more
semanticscholar +1 more source
The Bombyx mori nucleopolyhedrovirus (BmNPV) baculovirus expression system (BES) is a eukaryotic expression system. It possesses great capability for post‐translation modification in expression of foreign proteins. With the counterselection cassette rpsL‐
Xingjian Liu +6 more
doaj +1 more source

