Results 31 to 40 of about 93,476 (270)

Surface displaying of swine IgG1 Fc enhances baculovirus-vectored vaccine efficacy by facilitating viral complement escape and mammalian cell transduction

open access: yesVeterinary Research, 2017
Baculovirus-mediated gene transfer has been developed as a vaccine design strategy against a number of diseases without apparent viral replication. However, it has been hampered by complement-dependent inactivation, thus hindering the in vivo application
Zehui Liu   +6 more
doaj   +1 more source

Helicoverpa armigera nucleopolyhedrovirus occlusion-derived virus-associated protein, HA100, affects oral infectivity in vivo but not virus replication in vitro [PDF]

open access: yes, 2011
ORF100 (ha100) of Helicoverpa armigera nucleopolyhedrovirus (HearNPV) has been reported as one of the unique genes of group II alphabaculoviruses encoding a protein located in the occlusion-derived virus (ODV) envelope and nucleocapsid.
Deng, F.   +7 more
core   +3 more sources

Baculovirus as Versatile Vectors for Protein Display and Biotechnological Applications.

open access: yesCurrent Issues in Molecular Biology, 2019
The baculovirus-insect cell system has long been deployed for a variety of applications including for use as biopesticides, for recombinant protein production, transient transgene expression, tissue therapy, and for vaccine production.
Chih-Hsuan Tsai   +3 more
semanticscholar   +1 more source

Baculovirus as a vaccine vector [PDF]

open access: yesBioengineered, 2012
Baculovirus is extensively utilized as an excellent tool for production of recombinant protein in insect cells. Baculovirus infects insects in nature and is non-pathogenic to humans. In addition to insect cells, baculovirus is capable of transducing a broad range of animal cells.
Hsin-Yu, Lu   +2 more
openaire   +3 more sources

Fusion to green fluorescent protein improves expression levels of Theileria parva sporozoite surface antigen p67 in insect cells [PDF]

open access: yes, 2002
East Coast fever (ECF) is a fatal disease of cattle caused by the protozoan parasite Theileria parva. The development of a subunit vaccine, based on the sporozoite-specific surface antigen p67, has been hampered by difficulties in achieving high-level ...
Kaba, S.A.   +4 more
core   +2 more sources

MultiBac: Baculovirus-Mediated Multigene DNA Cargo Delivery in Insect and Mammalian Cells

open access: yesViruses, 2019
The baculovirus/insect cell system (BICS) is widely used in academia and industry to produce eukaryotic proteins for many applications, ranging from structure analysis to drug screening and the provision of protein biologics and therapeutics.
K. Gupta   +5 more
semanticscholar   +1 more source

Baculovirus Surface Display Using Infuenza Neuraminidase (NA) Transmembrane Anchor

open access: yesJournal of Tropical Life Science, 2016
Baculovirus surface display has been employed as an excellent tools for presentation of foreign peptides and proteins on virus surface with native conformation, functions and immunogenicity. A baculovirus major envelope protein, gp64, or a capsid protein,
Irisa Trianti   +3 more
doaj   +1 more source

A construction strategy for a baculovirus‐silkworm multigene expression system and its application for coexpression of type I and type II interferons

open access: yesMicrobiologyOpen, 2020
The Bombyx mori nucleopolyhedrovirus (BmNPV) baculovirus expression system (BES) is a eukaryotic expression system. It possesses great capability for post‐translation modification in expression of foreign proteins. With the counterselection cassette rpsL‐
Xingjian Liu   +6 more
doaj   +1 more source

Recent Developments in the Use of Baculovirus Expression Vectors.

open access: yesCurrent Issues in Molecular Biology, 2019
Over 35 years since it was established to make recombinant proteins, the baculovirus expression vector system continues to develop and improve. Early systems for recombinant virus selection were laborious, but better methods were rapidly devised that ...
R. Possee   +4 more
semanticscholar   +1 more source

Use of the piggyBac transposon to create HIV-1 gag transgenic insect cell lines for continuous VLP production

open access: yesBMC Biotechnology, 2010
Background Insect baculovirus-produced Human immunodeficiency virus type 1 (HIV-1) Gag virus-like-particles (VLPs) stimulate good humoral and cell-mediated immune responses in animals and are thought to be suitable as a vaccine candidate.
Shephard Enid G   +5 more
doaj   +1 more source

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