Results 11 to 20 of about 52,750 (225)

Purification of baculovirus vectors using heparin affinity chromatography

Molecular Therapy: Methods & Clinical Development, 2016
Baculoviruses are commonly used for recombinant protein and vaccine production. Baculoviruses are nonpathogenic to vertebrates, have a large packaging capacity, display broad host and cell type tropism, infect both dividing and nondividing cells, and do ...
Md Nasimuzzaman, Danielle Lynn, Johannes CM van der Loo, Punam Malik   +3 more
doaj   +2 more sources

Regulation of the prostate-specific hTGP (TGM4) gene and its potential use for prostate cancer gene therapy [PDF]

, 2011
Prostate cancer is a very important health problem in the UK and worldwide. Available treatments such as radiotherapy, surgery and androgen ablation have failed to significantly increase the life expectancy of prostate cancer patients.
Rivera Gonzalez, Guillermo C
core   +6 more sources

Baculovirus Per Os Infectivity Factors Form a Complex on the Surface of Occlusion-Derived Virus [PDF]

, 2010
Five highly conserved per os infectivity factors, PIF1, PIF2, PIF3, PIF4, and P74, have been reported to be essential for oral infectivity of baculovirus occlusion-derived virus (ODV) in insect larvae.
Hu, Z., van Oers, M.M., Vlak, J.M., van Lent, J.W.M., Lent, J.W.M., van, Peng, K., Oers, M.M., van   +6 more
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Pseudotyping Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV): F Proteins from Group II NPVs are functionally analogous to AcMNPV GP64 [PDF]

, 2002
GP64, the major envelope glycoprotein of budded virions of the baculovirus Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV), is involved in viral attachment, mediates membrane fusion during virus entry, and is required for efficient ...
Marcel Westenberg, Lung, O., Gary W. Blissard, Westenberg, M., Just M. Vlak, Douwe Zuidema, Vlak, J.M., Zuidema, D., Blissard, G.W., Oliver Lung   +9 more
core   +1 more source

Quantitative proteomics of Spodoptera frugiperda cells during growth and baculovirus infection. [PDF]

PLoS ONE, 2011
Baculovirus infection of Spodoptera frugiperda cells is a system of choice to produce a range of recombinant proteins, vaccines and, potentially, gene therapy vectors. While baculovirus genomes are well characterized, the genome of S.
Nuno Carinhas, Aaron Mark Robitaille, Suzette Moes, Manuel José Teixeira Carrondo, Paul Jenoe, Rui Oliveira, Paula Marques Alves   +6 more
doaj   +1 more source

A Novel Betabaculovirus Isolated from the Monocot Pest Mocis latipes (Lepidoptera: Noctuidae) and the Evolution of Multiple-Copy Genes

Viruses, 2018
In this report, we described the genome of a novel baculovirus isolated from the monocot insect pest Mocis latipes, the striped grass looper. The genome has 134,272 bp in length with a G + C content of 38.3%.
Daniel M. P. Ardisson-Araújo, Ana Maria Rodrigues da Silva, Fernando L. Melo, Ethiane Rozo dos Santos, Daniel R. Sosa-Gómez, Bergmann M. Ribeiro   +5 more
doaj   +1 more source

Functional processing and secretion of Chikungunya virus E1 and E2 glycoproteins in insect cells [PDF]

, 2011
Background: Chikungunya virus (CHIKV) is a mosquito-borne, arthrogenic Alphavirus that causes large epidemics in Africa, South-East Asia and India. Recently, CHIKV has been transmitted to humans in Southern Europe by invading and now established Asian ...
Geertsema, C (Corinne), Corinne Geertsema, van Oers, MM, Paulina Andrade, Heldens, J.G.M., Geertsema, C., Vlak Just M, Pijlman Gorben P, Andrade, Paulina, Vlak, J.M. (Just), Vlak, J.M., Pijlman, G.P. (Gorben), Just M Vlak, Metz Stefan W, Goldbach, RW, Metz, S.W. (Stefan), Martina, B.E.E. (Byron), Geertsema Corinne, Metz, S.W.H., Stefan W Metz, Jacco G Heldens, Geertsema, C. (Corinne), Heldens Jacco G, Martina, Byron, Andrade, P. (Paulina), Metz, SW, Andrade Paulina, Heldens, J., Rob W Goldbach, Pijlman, G.P., Pijlman, GP, van Oers Monique M, Oers, M.M. (Monique) van, van Oers, M.M., Andrade, P, Goldbach, R.W., Gorben P Pijlman, Monique M van Oers, Vlak, JM, Goldbach Rob W, Heldens, JG, Byron E Martina, Martina, Byron E., Martina Byron E, Oers, M.M., van   +44 more
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DNA photolyases of Chrysodeixis chalcites nucleopolyhedrovirus are targeted to the nucleus and interact with chromosomes and mitotic spindle structures [PDF]

, 2010
Cyclobutane pyrimidine dimer (CPD) photolyases convert UV-induced CPDs in DNA into monomers using visible light as the energy source. Two phr genes encoding class II CPD photolyases PHR1 and PHR2 have been identified in Chrysodeixis chalcites ...
Xu, F. (Fang), van Oers, MM, Oers, M.M. (Monique) van, van Oers, M.M., Vlak, J.M. (Just), Vlak, J.M., Eker, A.P.M., Vlak, JM, Xu, F., Xu, F, Oers, M.M., van, Eker, APM, Eker, A.P.M. (André)   +12 more
core   +1 more source

A rapid method for titrating baculovirus stocks using the Sf-9 Easy Titer cell line

BioTechniques, 2009
A new rapid method for titrating baculovirus stocks has been developed using a novel cell line Sf-9 Easy Titer (Sf-9ET). The Sf-9ET cell line has been transfected with plasmid DNA containing the enhanced green fluorescent protein (eGFP) gene under the ...
Ralph F. Hopkins, Dominic Esposito
doaj   +1 more source

Surface displaying of swine IgG1 Fc enhances baculovirus-vectored vaccine efficacy by facilitating viral complement escape and mammalian cell transduction

Veterinary Research, 2017
Baculovirus-mediated gene transfer has been developed as a vaccine design strategy against a number of diseases without apparent viral replication. However, it has been hampered by complement-dependent inactivation, thus hindering the in vivo application
Zehui Liu, Yangkun Liu, Yuanyuan Zhang, Yajuan Yang, Jingjing Ren, Xiaoying Zhang, Enqi Du   +6 more
doaj   +1 more source