Results 11 to 20 of about 93,476 (270)
Overview of the Baculovirus Expression System
Current Protocols in Protein Science, 2018 AbstractThis unit provides information on the replication cycle of insect baculovirus to provide an understanding of how this virus has been adapted for use as an expression vector for recombinant proteins in insect cells. We provide an overview of the virus structure and its unique bi‐phasic replication cycle, which has been exploited in developing ...
A. Chambers +5 more
semanticscholar +4 more sources
Viruses, 2023 Baculovirus expression system1s are a widely used tool in recombinant protein and biologics production. To enable the possibility of genome modifications unconstrained through low-throughput and bespoke classical genome manipulation techniques, we set ...
Christopher Nguyen +2 more
doaj +1 more source
Biotechnology Journal, 2020 Despite rapid progress in the field, scalable high‐yield production of adeno‐associated virus (AAV) is still one of the critical bottlenecks the manufacturing sector is facing. The insect cell‐baculovirus expression vector system (IC‐BEVS) has emerged as
P. Joshi +3 more
semanticscholar +1 more source
Clathrin-independent entry of baculovirus triggers uptake of E. coli in non-phagocytic human cells. [PDF]
PLoS ONE, 2009 The prototype baculovirus, Autographa californica multiple nucleopolyhedrovirus, an insect pathogen, holds great potential as a gene therapy vector. To develop transductional targeting and gene delivery by baculovirus, we focused on characterizing the ...
Johanna P Laakkonen +10 more
doaj +1 more source
Significant productivity improvement of the baculovirus expression vector system by engineering a novel expression cassette. [PDF]
PLoS ONE, 2014 Here we describe the development of a baculovirus vector expression cassette containing rearranged baculovirus-derived genetic regulatory elements. This newly designed expression cassette conferred significant production improvements to the baculovirus ...
Silvia Gómez-Sebastián +2 more
doaj +1 more source
Expression of functional recombinant human tissue transglutaminase (TG2) using the bac-to-bac baculovirus expression system [PDF]
, 2016 Purpose: Tissue transglutaminase (TG2) is a unique multifunctional enzyme. The enzyme possesses enzymatic activities such as transamidation/crosslinking and non-enzymatic functions such as cell migration and signal transduction.
Azari, S., Kalhor, H.R., Yazdani, Y.
core +2 more sources
Baculovirus Per Os Infectivity Factors Form a Complex on the Surface of Occlusion-Derived Virus [PDF]
, 2010 Five highly conserved per os infectivity factors, PIF1, PIF2, PIF3, PIF4, and P74, have been reported to be essential for oral infectivity of baculovirus occlusion-derived virus (ODV) in insect larvae.
Hu, Z. +4 more
core +2 more sources
Monodon Baculovirus of Shrimp [PDF]
Indian Journal of Virology, 2012 Among the viruses infecting penaeid shrimp, monodon-type baculovirus (MBV) otherwise known as Penaeus monodon singly enveloped nuclear polyhedrosis virus (PmSNPV), is one of the widely reported and well described viruses. It is a rod-shaped, enveloped, double-stranded DNA virus, and considered till recently, as the type A baculovirus.
K V, Rajendran +2 more
openaire +2 more sources
PLoS ONE, 2018 The human Adeno-Associated Virus serotype 2 (wtAAV2) is a common non-pathological virus and its recombinant form (rAAV) is widely used as gene therapy vector. Although rAAVs are routinely produced in the Baculovirus/Sf9 cell system, wtAAV2 has never been
Adrien Savy +5 more
doaj +1 more source
Quantitative proteomics of Spodoptera frugiperda cells during growth and baculovirus infection. [PDF]
PLoS ONE, 2011 Baculovirus infection of Spodoptera frugiperda cells is a system of choice to produce a range of recombinant proteins, vaccines and, potentially, gene therapy vectors. While baculovirus genomes are well characterized, the genome of S.
Nuno Carinhas +6 more
doaj +1 more source