Results 21 to 30 of about 52,750 (225)

Adjuvant-free immunization with hemagglutinin-Fc fusion proteins as an approach to influenza vaccines [PDF]

, 2011
The hemagglutinins (HAs) of human H1 and H3 influenza viruses and avian H5 influenza virus were produced as recombinant fusion proteins with the human immunoglobulin Fc domain.
Temperton, Nigel J., Ian M. Jones, Christopher R. Bailey, Eleonora Molesti, Ren, Junyuan, Jones, Ian M., Temperton, Nigel J, Silvia Loureiro, Pongsathon Phapugrangkul, Junyuan Ren, Wendy S. Barclay, Camilo A. Colaco, Molesti, Eleonora, Barclay, Wendy S., Shelton, Holly, Colaco, Camilo A, Phapugrangkul, Pongsathon, Nigel J. Temperton, Loureiro, Silvia, Barclay, Wendy S, Bailey, Christopher R, Holly Shelton, Jones, Ian, Colaco, Camilo A., Bailey, Christopher R.   +24 more
core   +1 more source

White spot syndrome virus envelope protein VP28 is involved in the systemic infection of shrimp [PDF]

, 2001
White spot syndrome virus (WSSV) is a large DNA virus infecting shrimp and other crustaceans. The virus particles contain at least five major virion proteins, of which three (VP26, VP24, and VP15) are present in the rod-shaped nucleocapsid and two (VP28 ...
van Hulten, Mariëlle C W, Vlak, Just M, Jeroen Witteveldt, Mariëlle C.W van Hulten, Marjolein Snippe, Witteveldt, Jeroen, Snippe, Marjolein, Just M Vlak, van Hulten, Mariëlle C.W   +8 more
core   +1 more source

A construction strategy for a baculovirus‐silkworm multigene expression system and its application for coexpression of type I and type II interferons

MicrobiologyOpen, 2020
The Bombyx mori nucleopolyhedrovirus (BmNPV) baculovirus expression system (BES) is a eukaryotic expression system. It possesses great capability for post‐translation modification in expression of foreign proteins. With the counterselection cassette rpsL‐
Xingjian Liu, Xin Yang, Arslan Mehboob, Xiaoyuan Hu, Yongzhu Yi, Yinü Li, Zhifang Zhang   +6 more
doaj   +1 more source

Baculovirus-Mediated Expression of Human 65 kDa and 67 kDa Glutamic Acid Decarboxylases in SF9 Insect Cells and Their Relevance in Diagnosis of Insulin-Dependent Diabetes Mellitus [PDF]

, 1993
cDNAs coding for the full-length human 65 and 67 kDa glutamic acid decarboxylases (GAD65 and GAD67) were amplified from pancreas and hippocampus cDNA libraries by polymerase chain reaction, respectively. Both cDNAs were inserted into a baculovirus vector
Cook, Neil J., Haubruck, Heinz, Seißler, Jochen, Abney, Charles C., Mauch, Ludwig, Liedvogel, Bodo, Wirbelauer, Christiane, Berthold, Heike, Scherbaum, Werner A., Northemann, Wolfgang   +9 more
core   +1 more source

Baculovirus Surface Display Using Infuenza Neuraminidase (NA) Transmembrane Anchor

Journal of Tropical Life Science, 2016
Baculovirus surface display has been employed as an excellent tools for presentation of foreign peptides and proteins on virus surface with native conformation, functions and immunogenicity. A baculovirus major envelope protein, gp64, or a capsid protein,
Irisa Trianti, Saengchai Akeprathumchai, Phenjun Mekvichitsaeng, Kanokwan Poomputsa   +3 more
doaj   +1 more source

Chemically Defined, High-Density Insect Cell-Based Expression System for Scalable AAV Vector Production

Molecular Therapy: Methods & Clinical Development, 2020
The recombinant adeno-associated virus (AAV) vector is one of the most utilized viral vectors in gene therapy due to its robust, long-term in vivo transgene expression and low toxicity.
James H. Kurasawa, Andrew Park, Carrie R. Sowers, Rebecca A. Halpin, Andrey Tovchigrechko, Claire L. Dobson, Albert E. Schmelzer, Changshou Gao, Susan D. Wilson, Yasuhiro Ikeda   +9 more
doaj   +1 more source

Improving baculovirus recombination [PDF]

Nucleic Acids Research, 2003
Recombinant baculoviruses have established themselves as a favoured technology for the high-level expression of recombinant proteins. The construction of recombinant viruses, however, is a time consuming step that restricts consideration of the technology for high throughput developments. Here we use a targeted gene knockout technology to inactivate an
Yuguang, Zhao, David A G, Chapman, Ian M, Jones   +2 more
openaire   +2 more sources

Use of the piggyBac transposon to create HIV-1 gag transgenic insect cell lines for continuous VLP production

BMC Biotechnology, 2010
Background Insect baculovirus-produced Human immunodeficiency virus type 1 (HIV-1) Gag virus-like-particles (VLPs) stimulate good humoral and cell-mediated immune responses in animals and are thought to be suitable as a vaccine candidate.
Shephard Enid G, Fraser Malcolm J, Tanzer Fiona, Lynch Alisson G, Williamson Anna-Lise, Rybicki Edward P   +5 more
doaj   +1 more source

A 96-well format for a high-throughput baculovirus generation, fast titering and recombinant protein production in insect and mammalian cells

BMC Research Notes, 2009
Background Baculovirus expression vector system (BEVS) has become a standard in recombinant protein production and virus-like particle preparation for numerous applications.
Roschier Miia M, Mähönen Anssi J, Toivanen Pyry I, Määttä Antti I, Lesch Hanna P, Kärkkäinen Hanna-Riikka, Airenne Kari J, Laitinen Olli H, Ylä-Herttuala Seppo   +8 more
doaj   +1 more source

Controlled and customizable baculovirus NOS3 gene delivery using PVA-based hydrogel systems.

PLoS ONE, 2023
Nitric oxide synthase 3 (NOS3) eluting polyvinyl alcohol-based hydrogels have a large potential in medical applications and device coatings. NOS3 promotes nitric oxide and nitrate production and can effectively be delivered using insect cell viruses ...
Sabrina Schaly, Paromita Islam, Jacqueline L Boyajian, Rahul Thareja, Ahmed Abosalha, Karan Arora, Dominique Shum-Tim, Satya Prakash   +7 more
doaj   +1 more source