Results 21 to 30 of about 68,162 (268)

Genetics instability of wtAAV2 genome and AAV promoter activities in the Baculovirus/Sf9 cells system.

PLoS ONE, 2018
The human Adeno-Associated Virus serotype 2 (wtAAV2) is a common non-pathological virus and its recombinant form (rAAV) is widely used as gene therapy vector. Although rAAVs are routinely produced in the Baculovirus/Sf9 cell system, wtAAV2 has never been
Adrien Savy, Minna U Kaikkonen, Adrien Léger, Yohann Dickx, Lionel Galibert, Otto-Wilhelm Merten   +5 more
doaj   +1 more source

Quantitative proteomics of Spodoptera frugiperda cells during growth and baculovirus infection. [PDF]

PLoS ONE, 2011
Baculovirus infection of Spodoptera frugiperda cells is a system of choice to produce a range of recombinant proteins, vaccines and, potentially, gene therapy vectors. While baculovirus genomes are well characterized, the genome of S.
Nuno Carinhas, Aaron Mark Robitaille, Suzette Moes, Manuel José Teixeira Carrondo, Paul Jenoe, Rui Oliveira, Paula Marques Alves   +6 more
doaj   +1 more source

Improving baculovirus recombination [PDF]

Nucleic Acids Research, 2003
Recombinant baculoviruses have established themselves as a favoured technology for the high-level expression of recombinant proteins. The construction of recombinant viruses, however, is a time consuming step that restricts consideration of the technology for high throughput developments. Here we use a targeted gene knockout technology to inactivate an
Yuguang, Zhao, David A G, Chapman, Ian M, Jones   +2 more
openaire   +2 more sources

Genome-wide diversity in temporal and regional populations of the betabaculovirus Erinnyis ello granulovirus (ErelGV)

BMC Genomics, 2018
Background Erinnyis ello granulovirus (ErelGV) is a betabaculovirus infecting caterpillars of the sphingid moth E. ello ello (cassava hornworm), an important pest of cassava crops (Manihot esculenta).
A. F. Brito, F. L. Melo, D. M. P. Ardisson-Araújo, W. Sihler, M. L. Souza, B. M. Ribeiro   +5 more
doaj   +1 more source

The baculovirus uses a captured host phosphatase to induce enhanced locomotory activity in host caterpillars. [PDF]

PLoS Pathogens, 2012
The baculovirus is a classic example of a parasite that alters the behavior or physiology of its host so that progeny transmission is maximized. Baculoviruses do this by inducing enhanced locomotory activity (ELA) that causes the host caterpillars to ...
Susumu Katsuma, Yasue Koyano, Wonkyung Kang, Ryuhei Kokusho, Shizuo George Kamita, Toru Shimada   +5 more
doaj   +1 more source

A Novel Betabaculovirus Isolated from the Monocot Pest Mocis latipes (Lepidoptera: Noctuidae) and the Evolution of Multiple-Copy Genes

Viruses, 2018
In this report, we described the genome of a novel baculovirus isolated from the monocot insect pest Mocis latipes, the striped grass looper. The genome has 134,272 bp in length with a G + C content of 38.3%.
Daniel M. P. Ardisson-Araújo, Ana Maria Rodrigues da Silva, Fernando L. Melo, Ethiane Rozo dos Santos, Daniel R. Sosa-Gómez, Bergmann M. Ribeiro   +5 more
doaj   +1 more source

Improvement of baculovirus as protein expression vector and as biopesticide by CRISPR/Cas9 editing

bioRxiv, 2019
The CRISPR (Clustered Regularly Interspaced Short Palindromic repeats) system associated Cas9 endonuclease is a molecular tool that enables specific sequence edition with high efficiency.
Chengchao Zhu, Jiuzhou Chen, Yu Wang, Lixian Wang, Xuan Guo, N. Chen, Ping Zheng, Jibin Sun, Yanhe Ma   +8 more
semanticscholar   +1 more source

Functional processing and secretion of Chikungunya virus E1 and E2 glycoproteins in insect cells [PDF]

, 2011
Background: Chikungunya virus (CHIKV) is a mosquito-borne, arthrogenic Alphavirus that causes large epidemics in Africa, South-East Asia and India. Recently, CHIKV has been transmitted to humans in Southern Europe by invading and now established Asian ...
Geertsema, C (Corinne), Corinne Geertsema, van Oers, MM, Paulina Andrade, Heldens, J.G.M., Geertsema, C., Vlak Just M, Pijlman Gorben P, Andrade, Paulina, Vlak, J.M. (Just), Vlak, J.M., Pijlman, G.P. (Gorben), Just M Vlak, Metz Stefan W, Goldbach, RW, Metz, S.W. (Stefan), Martina, B.E.E. (Byron), Geertsema Corinne, Metz, S.W.H., Stefan W Metz, Jacco G Heldens, Geertsema, C. (Corinne), Heldens Jacco G, Martina, Byron, Andrade, P. (Paulina), Metz, SW, Andrade Paulina, Heldens, J., Rob W Goldbach, Pijlman, G.P., Pijlman, GP, van Oers Monique M, Oers, M.M. (Monique) van, van Oers, M.M., Andrade, P, Goldbach, R.W., Gorben P Pijlman, Monique M van Oers, Vlak, JM, Goldbach Rob W, Heldens, JG, Byron E Martina, Martina, Byron E., Martina Byron E, Oers, M.M., van   +44 more
core   +1 more source

Baculovirus Entry and Egress from Insect Cells.

Annual Review of Virology, 2018
Baculoviruses are large DNA viruses of insects that are highly pathogenic in many hosts. In the infection cycle, baculoviruses produce two types of virions. These virion phenotypes are physically and functionally distinct, and each serves a critical role
G. Blissard, D. Theilmann
semanticscholar   +1 more source

White spot syndrome virus envelope protein VP28 is involved in the systemic infection of shrimp [PDF]

, 2001
White spot syndrome virus (WSSV) is a large DNA virus infecting shrimp and other crustaceans. The virus particles contain at least five major virion proteins, of which three (VP26, VP24, and VP15) are present in the rod-shaped nucleocapsid and two (VP28 ...
van Hulten, Mariëlle C W, Vlak, Just M, Jeroen Witteveldt, Mariëlle C.W van Hulten, Marjolein Snippe, Witteveldt, Jeroen, Snippe, Marjolein, Just M Vlak, van Hulten, Mariëlle C.W   +8 more
core   +1 more source