Results 41 to 50 of about 68,162 (268)

Chemically Defined, High-Density Insect Cell-Based Expression System for Scalable AAV Vector Production

Molecular Therapy: Methods & Clinical Development, 2020
The recombinant adeno-associated virus (AAV) vector is one of the most utilized viral vectors in gene therapy due to its robust, long-term in vivo transgene expression and low toxicity.
James H. Kurasawa, Andrew Park, Carrie R. Sowers, Rebecca A. Halpin, Andrey Tovchigrechko, Claire L. Dobson, Albert E. Schmelzer, Changshou Gao, Susan D. Wilson, Yasuhiro Ikeda   +9 more
doaj   +1 more source

Baculovirus and Insect Cell Gene Expression: Review of Baculovirus Biotechnology [PDF]

Environmental Health Perspectives, 1995
The BEVS continues to evolve as a powerful, flexible tool for molecular biology, protein function, and biomedical research. Future developments offer the promise of replacement of hazardous chemical insecticides with environmentally safe biopesticides, construction of baculovirus vectors which encode genes for specific post-translational modifications,
R M, Patterson, J K, Selkirk, B A, Merrick   +2 more
openaire   +2 more sources

FlexiBAC: a versatile, open-source baculovirus vector system for protein expression, secretion, and proteolytic processing

bioRxiv, 2018
BackgroundBaculovirus-mediated expression in insect cells is a powerful approach for protein production. However, many existing methods are time-consuming, offer limited options for protein tagging, and are unsuitable for secreted proteins requiring ...
Régis P. Lemaitre, Aliona Bogdanova, B. Borgonovo, Jeffrey B. Woodruff, D. Drechsel   +4 more
semanticscholar   +1 more source

Zika Virus Baculovirus-Expressed Virus-Like Particles Induce Neutralizing Antibodies in Mice

Virologica Sinica, 2018
The newly emerged mosquito-borne Zika virus (ZIKV) strains pose a global challenge owing to its ability to cause microcephaly and neurological disorders.
Shiyu Dai, Zhang Tao, Yanfang Zhang, Huálín Wáng, F. Dèng   +4 more
semanticscholar   +1 more source

A 96-well format for a high-throughput baculovirus generation, fast titering and recombinant protein production in insect and mammalian cells

BMC Research Notes, 2009
Background Baculovirus expression vector system (BEVS) has become a standard in recombinant protein production and virus-like particle preparation for numerous applications.
Roschier Miia M, Mähönen Anssi J, Toivanen Pyry I, Määttä Antti I, Lesch Hanna P, Kärkkäinen Hanna-Riikka, Airenne Kari J, Laitinen Olli H, Ylä-Herttuala Seppo   +8 more
doaj   +1 more source

Baculovirus Per Os Infectivity Factors Form a Complex on the Surface of Occlusion-Derived Virus

, 2010
Five highly conserved per os infectivity factors, PIF1, PIF2, PIF3, PIF4, and P74, have been reported to be essential for oral infectivity of baculovirus occlusion-derived virus (ODV) in insect larvae.
Hu, Z., van Oers, M.M., Vlak, J.M., van Lent, J.W.M., Lent, J.W.M., van, Peng, K., Oers, M.M., van   +6 more
core   +1 more source

Baculovirus Production

, 2023
Baculoviruses are arthropod-specific viruses containing large double-stranded circular DNA genomes of 80,000–180,000 bp. In recent years, they have been utilized for producing complex eukaryotic proteins in insect cell cultures.
Marcia Brady
core   +1 more source

Controlled and customizable baculovirus NOS3 gene delivery using PVA-based hydrogel systems.

PLoS ONE, 2023
Nitric oxide synthase 3 (NOS3) eluting polyvinyl alcohol-based hydrogels have a large potential in medical applications and device coatings. NOS3 promotes nitric oxide and nitrate production and can effectively be delivered using insect cell viruses ...
Sabrina Schaly, Paromita Islam, Jacqueline L Boyajian, Rahul Thareja, Ahmed Abosalha, Karan Arora, Dominique Shum-Tim, Satya Prakash   +7 more
doaj   +1 more source

Monobac System–A Single Baculovirus for the Production of rAAV

Microorganisms, 2021
Large-scale manufacturing of rAAV is a bottleneck for the development of genetic disease treatments. The baculovirus/Sf9 cell system underpins the first rAAV treatment approved by EMA and remains one of the most advanced platforms for rAAV manufacturing.
Lionel Galibert, Aurélien Jacob, Adrien Savy, Yohann Dickx, Delphine Bonnin, Christophe Lecomte, Lise Rivollet, Peggy Sanatine, Marjorie Boutin Fontaine, Christine Le Bec, Otto-Wilhelm Merten   +10 more
doaj   +1 more source

Baculovirus Kimura two-parameter species demarcation criterion is confirmed by the distances of 38 core gene nucleotide sequences.

Journal of General Virology, 2018
Kimura two-parameter nucleotide distance comparisons based on polyhedrin/granulin (polh/gran), late expression factor 8 (lef-8) and late expression factor 9 (lef-9) are a widely applied method for species demarcation for lepidopteran-specific ...
J. Wennmann, J. Keilwagen, J. Jehle
semanticscholar   +1 more source