Results 41 to 50 of about 93,476 (270)

Cytoplasmic islet cell antibodies recognize distinct islet antigens in IDDM but not in stiff man syndrome [PDF]

open access: yes, 1993
Cytoplasmic islet cell antibodies are well-established predictive markers of IDDM. Although target molecules of ICA have been suggested to be gangliosides, human monoclonal ICA of the immunoglobulin G class (MICA 1-6) produced from a patient with newly ...
Meinck, Hans-Michael   +5 more
core   +1 more source

Baculovirus and Insect Cell Gene Expression: Review of Baculovirus Biotechnology [PDF]

open access: yesEnvironmental Health Perspectives, 1995
The BEVS continues to evolve as a powerful, flexible tool for molecular biology, protein function, and biomedical research. Future developments offer the promise of replacement of hazardous chemical insecticides with environmentally safe biopesticides, construction of baculovirus vectors which encode genes for specific post-translational modifications,
R M, Patterson   +2 more
openaire   +2 more sources

Chemically Defined, High-Density Insect Cell-Based Expression System for Scalable AAV Vector Production

open access: yesMolecular Therapy: Methods & Clinical Development, 2020
The recombinant adeno-associated virus (AAV) vector is one of the most utilized viral vectors in gene therapy due to its robust, long-term in vivo transgene expression and low toxicity.
James H. Kurasawa   +9 more
doaj   +1 more source

The Nuclease Activity of the Yeast Dna2 Protein, Which Is Related to the RecB-like Nucleases, Is Essential in Vivo [PDF]

open access: yes, 2000
Saccharomyces cerevisiae Dna2 protein is required for DNA replication and repair and is associated with multiple biochemical activities: DNA-dependent ATPase, DNA helicase, and DNA nuclease.
Budd, Martin E.   +2 more
core   +1 more source

Zika Virus Baculovirus-Expressed Virus-Like Particles Induce Neutralizing Antibodies in Mice

open access: yesVirologica Sinica, 2018
The newly emerged mosquito-borne Zika virus (ZIKV) strains pose a global challenge owing to its ability to cause microcephaly and neurological disorders.
Shiyu Dai   +4 more
semanticscholar   +1 more source

Comparative Evaluation of Human Papillomavirus Type 16 L1 Protein Expressed in Plasmid- and Baculovirus-Based Systems in Insect Cells [PDF]

open access: yesArchives of Razi Institute, 2020
Human papillomavirus (HPV) has been associated with specific types of papillomas, lesions at particular anatomic sites, and malignancies. The HPV16 and HPV18 have been shown to play a role in a variety of carcinomas.
B. Abedi Kiasari
doaj   +1 more source

Gad65 is recognized by t-cells, but not by antibodies from nod-mice [PDF]

open access: yes, 1994
Since the 64kDa-protein glutamic acid decarboxylase (GAD) is one of the major autoantigens in T-cell mediated Type 1 diabetes, its relevance as a T-cell antigen needs to be clarified. After isolation of splenic T-cells from non-obese diabetic (NOD) mice,
Alzari PM   +28 more
core   +1 more source

FlexiBAC: a versatile, open-source baculovirus vector system for protein expression, secretion, and proteolytic processing

open access: yesbioRxiv, 2018
BackgroundBaculovirus-mediated expression in insect cells is a powerful approach for protein production. However, many existing methods are time-consuming, offer limited options for protein tagging, and are unsuitable for secreted proteins requiring ...
Régis P. Lemaitre   +4 more
semanticscholar   +1 more source

Identificación de un aislamiento argentino de Spodoptera frugiperda granulovirus [PDF]

open access: yes, 2019
La oruga militar tardía, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae), es una plaga importante del maíz. Debido al impacto ambiental y a la aparición de resistencia causados por los pesticidas químicos y los eventos transgénicos, el uso de
Ferrelli, Maria Leticia   +3 more
core   +2 more sources

A 96-well format for a high-throughput baculovirus generation, fast titering and recombinant protein production in insect and mammalian cells

open access: yesBMC Research Notes, 2009
Background Baculovirus expression vector system (BEVS) has become a standard in recombinant protein production and virus-like particle preparation for numerous applications.
Roschier Miia M   +8 more
doaj   +1 more source

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