Results 61 to 70 of about 93,476 (270)

Anti-tumor activity of Escherichia coli Shiga toxin A subunit delivered by SF9 insect cells

Journal of Pharmacological Sciences, 2018
Cancer remains a major health problem around the world. A Shiga toxin is a bacterial toxin often produced by Shigella dysenteriae and Escherichia coli. A subunit of the Shiga toxin (StxA) is a cytotoxic agent which could be used to induce death in cancer
Mana Oloomi, Maryam Imani, Ramezan Behzadi, Mohsen Asori, Saeid Bouzari, Bita Mokhlesi   +5 more
doaj   +1 more source

A Mammalian Homolog of Drosophila melanogaster Transcriptional Coactivator Intersex Is a Subunit of the Mammalian Mediator Complex [PDF]

, 2003
The multiprotein Mediator complex is a coactivator required for transcriptional activation of RNA polymerase II transcribed genes by DNA binding transcription factors.
Banks, Charles A. S., Brower, Christopher S., Conaway, Joan Weliky, Conaway, Ronald C., Parmely, Tari J., Sato, Shigeo, Sorokina, Irina, Tomomori-Sato, Chieri   +7 more
core   +1 more source

A new single-step protocol for rapid baculovirus-driven protein production in insect cells

BMC Biotechnology, 2017
BackgroundIn the last three decades, the Baculovirus expression vector system (BEV) has evolved to one of the most widely used eukaryotic systems for heterologous protein expression including approved vaccines and therapies.
Judith D. Scholz, S. Suppmann
semanticscholar   +1 more source

Protocol for Reconstituting Adaptor‐Mediated Activation of Full‐Length Kinesin‐1

Cytoskeleton, EarlyView.
ABSTRACT Kinesin‐1 is a member of the kinesin superfamily that plays an essential role in intracellular cargo transport. In the absence of cargo, Kinesin‐1 exhibits low motor activity due to autoinhibition. Multiple studies have demonstrated that adaptor proteins, which link cargos to Kinesin‐1, can activate Kinesin‐1 by releasing the autoinhibition ...
Haruka Masumoto, Kyoko Chiba
wiley   +1 more source

Residual baculovirus in insect cell-derived influenza virus-like particle preparations enhances immunogenicity.

PLoS ONE, 2012
Influenza virus-like particles are currently evaluated in clinical trials as vaccine candidates for influenza viruses. Most commonly they are produced in baculovirus- or mammalian- expression systems.
Irina Margine, Luis Martinez-Gil, Yi-Ying Chou, Florian Krammer   +3 more
doaj   +1 more source

Baculovirus Surface Display of Immunogenic Proteins for Vaccine Development

Viruses, 2018
Vaccination is an efficient way to prevent the occurrence of many infectious diseases in humans. To date, several viral vectors have been utilized for the generation of vaccines.
B. Premanand, Poh Zhong Wee, Mookkan Prabakaran   +2 more
semanticscholar   +1 more source

The Influence of Drosophila Spire and Myosin V During Mid‐Oogenesis Is Independent of Their Direct Interaction

Cytoskeleton, EarlyView.
ABSTRACT Cooperativity between cytoskeletal proteins is crucial for spatiotemporal coordination in biological processes, like oogenesis. In mammalian and Drosophila oogenesis, proper assembly and function of actin networks require coordination between actin assembly factors Spire and formins, as well as actin‐associated proteins like myosins and Rab ...
Joseph Y. Ong, Hannah M. Bailey, Emma Carley, Michelle Panzica, Merin Rixen, Neil Billington, Rong Liu, Margot E. Quinlan   +7 more
wiley   +1 more source

Improving the baculovirus expression vector system with vankyrin‐enhanced technology

Biotechnology progress (Print), 2017
The baculovirus expression vector system (BEVS) is a widely used platform for the production of recombinant eukaryotic proteins. However, the BEVS has limitations in comparison to other higher eukaryotic expression systems.
Kendra H Steele, Barbara J. Stone, K. M. Franklin, A. Fath-Goodin, Xiufeng Zhang, Haobo Jiang, B. Webb, C. Geisler   +7 more
semanticscholar   +1 more source

Expression, Purification, and Microscopy‐Based Assays for Engineered Recombinant Tyrosinated, Detyrosinated, and Δ2 Human Tubulin

Cytoskeleton, EarlyView.
ABSTRACT Microtubules are noncovalent polymers assembled from α/β tubulin dimers. Their structure, dynamics and interaction with effectors are regulated through the expression of diverse tubulin isotypes and chemically diverse posttranslational modifications, also known as the “tubulin code.” Understanding the biophysical correlates between tubulin ...
Jiayi Chen, Agnieska Szyk, Antonina Roll‐Mecak   +2 more
wiley   +1 more source

Original Article. Topical treatment of LdMNPV-infected gypsy moth caterpillars with 18 nucleotides long antisense fragment from LdMNPV IAP3 gene triggers higher levels of apoptosis in infected cells and mortality of the pest

Journal of Plant Protection Research, 2016
The high efficiency of baculovirus infection is partially explained by the ability of the virus to suppress host defense machinery connected with the apoptosis pathway.
Oberemok Volodymyr V., Laikova Kateryna V., Zaitsev Alexey S., Nyadar Palmah M., Gninenko Yuri I., Gushchin Vladimir A., Makarov Valentin V., Agranovsky Alexey A.   +7 more
doaj   +1 more source