Results 61 to 70 of about 68,162 (268)

What Makes an “Ideal” Cell Line for Recombinant Adeno‐Associated Virus Production?

Biotechnology and Bioengineering, EarlyView.
Several host cell types have been used to produce rAAVs to date. Cell line‐specific traits that are beneficial in the context of rAAV manufacturing are reviewed here, with the goal of developing a consensus on the ‘ideal’ characteristics that an rAAV production host should possess (created with Biorender.com).
James Conheady, Nicholas Donohue, Alexandra Bogdanovic, Sharon Davin, Brian Glennon, Niall Barron, C. Mark Smales   +6 more
wiley   +1 more source

Baculovirus Surface Display of Immunogenic Proteins for Vaccine Development

Viruses, 2018
Vaccination is an efficient way to prevent the occurrence of many infectious diseases in humans. To date, several viral vectors have been utilized for the generation of vaccines.
B. Premanand, Poh Zhong Wee, Mookkan Prabakaran   +2 more
semanticscholar   +1 more source

Baculovirus-Mediated Expression of Human 65 kDa and 67 kDa Glutamic Acid Decarboxylases in SF9 Insect Cells and Their Relevance in Diagnosis of Insulin-Dependent Diabetes Mellitus [PDF]

, 1993
cDNAs coding for the full-length human 65 and 67 kDa glutamic acid decarboxylases (GAD65 and GAD67) were amplified from pancreas and hippocampus cDNA libraries by polymerase chain reaction, respectively. Both cDNAs were inserted into a baculovirus vector
Cook, Neil J., Haubruck, Heinz, Seißler, Jochen, Abney, Charles C., Mauch, Ludwig, Liedvogel, Bodo, Wirbelauer, Christiane, Berthold, Heike, Scherbaum, Werner A., Northemann, Wolfgang   +9 more
core  

Anti-tumor activity of Escherichia coli Shiga toxin A subunit delivered by SF9 insect cells

Journal of Pharmacological Sciences, 2018
Cancer remains a major health problem around the world. A Shiga toxin is a bacterial toxin often produced by Shigella dysenteriae and Escherichia coli. A subunit of the Shiga toxin (StxA) is a cytotoxic agent which could be used to induce death in cancer
Mana Oloomi, Maryam Imani, Ramezan Behzadi, Mohsen Asori, Saeid Bouzari, Bita Mokhlesi   +5 more
doaj   +1 more source

Status and future of recombinant adeno‐associated virus vector manufacturing

Biotechnology Progress, EarlyView.
Abstract Sixty years of adeno‐associated virus (AAV) research illustrates a trajectory marked by basic science exploration, iterative innovation, persistent challenges, a number of clinical setbacks, as well as commercial therapeutic triumphs. This continual evolution has led to recombinant AAV (rAAV) becoming a cornerstone of modern gene therapy ...
Frank Agbogbo, David Dismuke
wiley   +1 more source

Improving the Baculovirus Expression Vector System with Vankyrin-Enhanced Technology

Biotechnology progress (Print), 2017
The baculovirus expression vector system (BEVS) is a widely used platform for the production of recombinant eukaryotic proteins. However, the BEVS has limitations in comparison to other higher eukaryotic expression systems.
Kendra H Steele, Barbara J. Stone, K. M. Franklin, A. Fath-Goodin, Xiufeng Zhang, Haobo Jiang, B. Webb, C. Geisler   +7 more
semanticscholar   +1 more source

Original Article. Topical treatment of LdMNPV-infected gypsy moth caterpillars with 18 nucleotides long antisense fragment from LdMNPV IAP3 gene triggers higher levels of apoptosis in infected cells and mortality of the pest

Journal of Plant Protection Research, 2016
The high efficiency of baculovirus infection is partially explained by the ability of the virus to suppress host defense machinery connected with the apoptosis pathway.
Oberemok Volodymyr V., Laikova Kateryna V., Zaitsev Alexey S., Nyadar Palmah M., Gninenko Yuri I., Gushchin Vladimir A., Makarov Valentin V., Agranovsky Alexey A.   +7 more
doaj   +1 more source

Protocol for Reconstituting Adaptor‐Mediated Activation of Full‐Length Kinesin‐1

Cytoskeleton, EarlyView.
ABSTRACT Kinesin‐1 is a member of the kinesin superfamily that plays an essential role in intracellular cargo transport. In the absence of cargo, Kinesin‐1 exhibits low motor activity due to autoinhibition. Multiple studies have demonstrated that adaptor proteins, which link cargos to Kinesin‐1, can activate Kinesin‐1 by releasing the autoinhibition ...
Haruka Masumoto, Kyoko Chiba
wiley   +1 more source

Replication of baculovirus DNA

Journal of General Virology, 1995
Introduction. The Baculoviridae is a diverse family of pathogens that are infectious for arthropods and are characterized by a complex replication cycle that culminates in the occlusion of virions in a crystalline protein matrix (Blissard & Rohrmann, 1990).
Kool, M., Ahrens, C.H., Vlak, J.M., Rohrmann, G.F.   +3 more
openaire   +3 more sources

Baculovirus-driven protein expression in insect cells: A benchmarking study.

Journal of Structural Biology, 2018
Baculovirus-insect cell expression system has become one of the most widely used eukaryotic expression systems for heterologous protein production in many laboratories.
Peggy Stolt-Bergner, C. Benda, Tim Bergbrede, H. Besir, P. Celie, Cindy Chang, D. Drechsel, A. Fischer, A. Geerlof, B. Giabbai, J. van den Heuvel, G. Huber, W. Knecht, Anita Lehner, Régis P. Lemaitre, K. Nordén, G. Pardee, I. Racke, K. Remans, A. Sander, Judith D. Scholz, M. Stadnik, P. Storici, Daniel Weinbruch, I. Zaror, L. Lua, S. Suppmann   +26 more
semanticscholar   +1 more source