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Baculovirus expression vectors
1993Abstract Progress in molecular virology has given us sufficient understanding of genetic organization and regulation in certain viruses of eukaryotes to enable us to utilize these systems as expression vectors of foreign genes. Analysis of the baculovirus genome has identified genes which are redundant when the virus is grown in cell ...
Lorna M D Stewart, Robert D Possee
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Baculovirus expression vectors.
Methods in molecular biology (Clifton, N.J.), 2012This chapter aims to provide the reader with the experimental protocols required to produce a baculovirus containing the gene of interest, including transfection of Spodoptera frugiperda cells and the screening of progeny virus by plaque assay. In addition, an outline of the biochemical methods routinely used to characterize the expressed protein ...
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