Results 31 to 40 of about 3,963,937 (304)

A high-throughput gene disruption methodology for the entomopathogenic fungus Metarhizium robertsii. [PDF]

open access: yesPLoS ONE, 2014
Systematic gene disruption is a direct way to interrogate a fungal genome to functionally characterize the full suite of genes involved in various biological processes.
Chuan Xu   +7 more
doaj   +1 more source

In vitro selection of transgenic soybean lines obtained from somatic embryos transformed with Agrobacterium tumefaciens

open access: yesBiotecnología Vegetal, 2021
Although genetic transformation in Glycine max has been intensively investigated, the process remains ineffective. In the present work, for the first time, the in vitro selection of transgenic lines of soybean variety Incasoy-27 is addressed.
Jorge L. Pérez Pérez   +3 more
doaj  

Comparative analysis of T4 DNA ligases and DNA polymerases used in chromosome conformation capture assays

open access: yesBioTechniques, 2015
Three-dimensional (3-D) genome organization in the nuclear space affects various genomic functions. Circular chromosome conformation capture (4C-seq) is a powerful technique that allows researchers to measure long-range chromosomal interactions with a ...
Michal Schwartz   +7 more
doaj   +1 more source

Herbicide Resistance of Tobacco Chloroplasts Expressing the bar Gene

open access: yesMolecules and Cells, 2003
The chloroplast transformation system has the potential advantages of maternal inheritance and high-level expression of heterologous genes. We studied the expression of the bar gene in tobacco chloroplasts to test these ideas. The bar gene conferring tolerance to the herbicide phosphinothricin (PPT) encodes phosphinothricin acetyltransferase (PAT).
Tae-Jin, Kang   +3 more
openaire   +2 more sources

Non-specific activities of the major herbicide-resistance gene BAR [PDF]

open access: yesNature Plants, 2017
Bialaphos resistance (BAR) and phosphinothricin acetyltransferase (PAT) genes, which convey resistance to the broad-spectrum herbicide phosphinothricin (also known as glufosinate) via N-acetylation, have been globally used in basic plant research and genetically engineered crops 1-4 .
Christ, Bastien   +6 more
openaire   +5 more sources

Efficient expression of heterologous genes by the introduction of the endogenous glyceraldehyde-3-phosphate dehydrogenase gene intron 1 in Ganoderma lucidum

open access: yesMicrobial Cell Factories, 2021
Background Ganoderma lucidum, a well-known medicinal mushroom, has received wide attention as a promising cell factory for producing bioactive compounds.
Hao You, Bin Sun, Na Li, Jun-Wei Xu
doaj   +1 more source

Genetic mapping of the sex-linked barring gene in the chicken

open access: yesPoultry Science, 2009
The sex-linked barring gene of the chicken (Gallus gallus), first identified in 1908, produces an alternating pattern of white and black bars in the adult plumage. More recent studies have shown that melanocytes in the developing feather follicle of the Barred Plymouth Rock experience premature cell death, whereas initially it was thought that ...
B J, Dorshorst, C M, Ashwell
openaire   +2 more sources

Construction of Paecilomyces fumosoroseus protoplast-transforming system by restriction enzyme-mediated integration (REMI)

open access: yes浙江大学学报. 农业与生命科学版, 2006
Phosphinothricin resistance gene Bar vectored by prepared plasmid pAN52-1N-Bar was transformed into the protoplasts of the entomopathogenic fungus Paecilomyces fumosoroseus Pfr116 using the method of restriction enzyme-mediated integration (REMI).
YANG Xu-fen   +2 more
doaj   +1 more source

Detection of bar transgenic sugarcane with a rapid and visual loop-mediated isothermal amplification assay

open access: yesFrontiers in Plant Science, 2016
Genetic engineering offers an attractive alternative in sugarcane breeding for increasing cane and sugar yields as well as disease and insect resistance.
Dinggang eZhou   +9 more
doaj   +1 more source

Hyperoxic treatment induces mesenchymal-to-epithelial transition in a rat adenocarcinoma model. [PDF]

open access: yesPLoS ONE, 2009
Tumor hypoxia is relevant for tumor growth, metabolism and epithelial-to-mesenchymal transition (EMT). We report that hyperbaric oxygen (HBO) treatment induced mesenchymal-to-epithelial transition (MET) in a dimethyl-alpha-benzantracene induced mammary ...
Ingrid Moen   +8 more
doaj   +1 more source

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