Results 51 to 60 of about 91,234 (296)

First Record Sparus aurata Larvae in Teluk Penyu Beach Cilacap, Indonesia, revealed by DNA Barcoding

Ilmu Kelautan, 2023
Morphological identification often fails to reveal the taxonomic status of fish larvae. DNA barcoding using particular DNA segment is frequently successful in solving the faulty and might reveal overlooked species, including species outside their ...
Agus Nuryanto, Dian Bhagawati, Elly Tuti Winarni, Hanan Hassan Alsheikh Mahmoud   +3 more
doaj   +1 more source

Analysing ambiguities in trypanosomatids taxonomy by barcoding [PDF]

Memorias do Instituto Oswaldo Cruz, 2020
BACKGROUND Biodiversity screens and phylogenetic studies are dependent on reliable DNA sequences in public databases. Biological collections possess vouchered specimens with a traceable history.
Carolina Boucinha, Amanda R Caetano, Helena LC Santos, Raphael Helaers, Miikka Vikkula, Marta Helena Branquinha, André Luis Souza dos Santos, Philippe Grellier, Karina Alessandra Morelli, Claudia Masini d‘Avila-Levy   +9 more
doaj   +1 more source

In vitro models of cancer‐associated fibroblast heterogeneity uncover subtype‐specific effects of CRISPR perturbations

Molecular Oncology, EarlyView.
Development of therapies targeting cancer‐associated fibroblasts (CAFs) necessitates preclinical model systems that faithfully represent CAF–tumor biology. We established an in vitro coculture system of patient‐derived pancreatic CAFs and tumor cell lines and demonstrated its recapitulation of primary CAF–tumor biology with single‐cell transcriptomics ...
Elysia Saputra, Shamsudheen Karuthedath Vellarikkal, Lixia Li, Hong Sun, Khoa Nguyen, Amber Montano, Suchitra Natarajan, Federica Piccioni, Alex Michael Tamburino, Xin Yu, Aleksandra Katarzyna Olow   +10 more
wiley   +1 more source

DNA barcoding and phylogenetic analysis of endemic Astragalus nezaketiae and Vicia alpestris subsp. hypoleuca (Fabaceae): Evidence from nrDNA ITS and cpDNA matK and rbcL sequences

Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 2022
In this study, we performed DNA barcoding and phylogenetic analysis using one nuclear (ITS) and two chloroplast DNA regions (matK and rbcL) of endemic Astragalus nezaketiae A. Duran & Aytaçand Vicia alpestris Stev. subsp.
Mehmet Y. PAKSOY, Emre SEVİNDİK, İsa BAŞKÖSE   +2 more
doaj   +1 more source

Developmental barcoding of whole mouse via homing CRISPR

Science, 2018
Lineage tracing in mouse using CRISPR A homing guide RNA (hgRNA) that directs CRISPR-Cas9 to its own DNA locus can diversify its sequence and act as an expressed genetic barcode. Kalhor et al.
Reza Kalhor, K. Kalhor, Leo Mejia, Kathleen Leeper, A. Graveline, P. Mali, G. Church   +6 more
semanticscholar   +1 more source

Dual targeting of RET and SRC synergizes in RET fusion‐positive cancer cells

Molecular Oncology, EarlyView.
Despite the strong activity of selective RET tyrosine kinase inhibitors (TKIs), resistance of RET fusion‐positive (RET+) lung cancer and thyroid cancer frequently occurs and is mainly driven by RET‐independent bypass mechanisms. Son et al. show that SRC TKIs significantly inhibit PAK and AKT survival signaling and enhance the efficacy of RET TKIs in ...
Juhyeon Son, Lily L. Remsing Rix, Bin Fang, Eric A. Welsh, Nicole V. Bremer, Valentina Foglizzo, Paola Roa, Nickole Sigcha‐Coello, Yi Liao, Eric B. Haura, Alexander Drilon, John M. Koomen, Emiliano Cocco, Uwe Rix   +13 more
wiley   +1 more source

Monitoring of circulating tumor DNA allows early detection of disease relapse in patients with operable breast cancer

Molecular Oncology, EarlyView.
Monitoring circulating tumor DNA (ctDNA) in patients with operable breast cancer can reveal disease relapse earlier than radiology in a subset of patients. The failure to detect ctDNA in some patients with recurrent disease suggests that ctDNA could serve as a supplement to other monitoring approaches.
Kristin Løge Aanestad, Marie Austdal, Oddmund Nordgård, Gunnar Mellgren, Satu Oltedal, Marie L. Austbø, Ylva H. Vignes, Thomas Helland, Kristin Jonsdottir, Tone H. Lende, Emilius A. M. Janssen, Bjørnar Gilje, Kjersti Tjensvoll, PBCB study group, Gunnar Mellgren, Tone Hoel Lende, Anette Heie, Kristin Viste, Anita Røyneberg Alvheim, Emiel AM Janssen, Kristin Jonsdottir, Ann Cathrine Kroksveen, Thomas Helland, Einar Gudlaugsson, Oddmund Nordgård, Satu Oltedal, Kristin Løge Aanestad, Jan Terje Kvaløy, Kirsten Lode, Kari Britt Hagen, Marie Austdal, Ylva H. Vignes, Siri Tungland Sola, Nina Egeland Amundsen, Finn Magnus Eliassen, Emeritus Ernst A Lien   +35 more
wiley   +1 more source

Takeaways from Mobile DNA Barcoding with BentoLab and MinION

Genes, 2020
Since the release of the MinION sequencer in 2014, it has been applied to great effect in the remotest and harshest of environments, and even in space. One of the most common applications of MinION is for nanopore-based DNA barcoding in situ for species ...
Jia Jin Marc Chang, Yin Cheong Aden Ip, C. L. Ng, Danwei Huang   +3 more
semanticscholar   +1 more source

ATF4‐mediated stress response as a therapeutic vulnerability in chordoma

Molecular Oncology, EarlyView.
We screened 5 chordoma cell lines against 100+ inhibitors of epigenetic and metabolic pathways and kinases and identified halofuginone, a tRNA synthetase inhibitor. Mechanistically halofuginone induces an integrated stress response, with eIF2alpha phosphorylation, activation of ATF4 and its target genes CHOP, ASNS, INHBE leading to cell death ...
Lucia Cottone, James Dunford, Eleanor Calcutt, Vicki Gamble, Filiz Senbabaoglu Aksu, Lorena Ligammari, Georgina Wherry, Giorgia Gaeta, John C. Christianson, Adrienne M. Flanagan, Udo Oppermann, Adam P. Cribbs   +11 more
wiley   +1 more source

New World dung beetle (Coleoptera: Scarabaeidae: Scarabaeinae) colonization of a recent Miocene insular territory: The case of Costa Rica

Ecology and Evolution
Costa Rica emerged from the seas as a new geological territory during the Miocene as an insular archipelago. It later became part of a continental area once it became a segment of Central America.
Bert Kohlmann, Renato Portela Salomão, Ángel Solís   +2 more
doaj   +1 more source