Results 51 to 60 of about 150,695 (267)

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential

FEBS Letters, EarlyView.
Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta, Hari Baskar Balasubramanian, Amir Pasokh, Marietta Zille   +3 more
wiley   +1 more source

$L_{1}$-Based Adaptive Identification With Saturated Observations

IEEE Transactions on Automatic Control
It is well-known that saturated output observations are prevalent in various practical systems and that the $\ell_1$-norm is more robust than the $\ell_2$-norm-based parameter estimation. Unfortunately, adaptive identification based on both saturated observations and the $\ell_1$-optimization turns out to be a challenging nonlinear problem, and has ...
Xin Zheng, Lei Guo 0001
openaire   +2 more sources

Autophagy and mitophagy in pancreatic β‐cell homeostasis and their involvement in diabetes pathophysiology

FEBS Letters, EarlyView.
This review focuses on the role of autophagy and mitophagy in maintaining pancreatic β‐cell function and homeostasis. We discuss how genetic defects affecting these pathways contribute to the development of type 1, type 2, monogenic, and gestational diabetes. We further explore their potential as therapeutic targets. Created in BioRender.
Yunkyeong Lee, Mingming Tong, Anna L. Gloyn   +2 more
wiley   +1 more source

The Reactions of Common Chemical Fertilizers in An Ultisol and Their Effects on Cowpea Performance

Journal of Tropical Soils, 2019
Chemical fertilizers (CF) are the major input on arable farms in Nigeria. Current increasing rate of nutrient depletion in CF treated soils calls for the need to study their reactions in soils. To achieve this, three CF based treatments, namely NPK 15:15:
Oyeyiola Yetunde Bunmi, Omueti John Ajayi   +1 more
doaj   +1 more source

Golgi enzymes are retrieved from the plasma membrane to the trans‐Golgi network

FEBS Letters, EarlyView.
Golgi enzymes are traditionally considered resident proteins retained within the Golgi apparatus. Here, we demonstrate that a subset transiently reaches the cell surface and is subsequently retrieved to the trans‐Golgi network via retrograde transport. Using a nanobody‐based toolkit, we uncover a dynamic trafficking cycle of several Golgi enzymes.
Dominik P. Buser, Tina Junne
wiley   +1 more source

Accuracy of methods to estimate potential acidity and lime requirement in soils of west region of Santa Catarina

Ciência Rural, 2018
: The objective of this study was to compare two methods of evaluation of potencial acidity (H+Al) and two methods to estimate liming requirement (LR) for soils from the west region of Santa Catarina State, Brazil.
Rodrigo Predebon, Luciano Colpo Gatiboni, Gilmar Luiz Mumbach, Djalma Eugenio Schmitt, Daniel João Dall’Orsoletta, Gustavo Brunetto   +5 more
doaj   +1 more source

A novel quinazolinone insulin receptor inhibitor and its synergy with an EGFR inhibitor in glucose‐driven glioblastoma

Molecular Oncology, EarlyView.
The novel styrylquinazolinone‐based molecule W1B effectively suppresses glioblastoma by inhibiting IGF1R and EGFR. In high‐glucose microenvironments driving tumor resistance, W1B acts synergistically with the EGFR inhibitor dacomitinib. This combination safely blocks compensatory survival signaling in zebrafish xenograft models. Showcasing promising in
Patryk Rurka, Wioleta Cieślik, Wojciech Płaziński, Katarzyna Stępnik, Anna Boguszewska‐Czubara, Elżbieta Kot, Robert Musioł, Mateusz Jasica, Anna Mrozek‐Wilczkiewicz, Katarzyna Malarz   +9 more
wiley   +1 more source

Soil acidity levels for blackberry liming recommendation and yield increase

Pesquisa Agropecuária Brasileira
The objective of this work was to determine the acidity levels to obtain adequate soil chemical attributes for the maximum fruit yield of blackberry trees grown in acidic soils subjected to increasing limestone rates.
Enilson de Barros Silva, Ramony Cristina Lima, Jéssica Oliveira Gaurat, Ari Medeiros Braga Neto, Maria do Céu Monteiro da Cruz, Dalila Costa Aparecida Bié, Emerson Dias Gonçalves, Luiz Fernando de Oliveira Silva, Pedro Henrique Abreu Moura, Shirley Macedo de Souza   +9 more
doaj   +1 more source