Base composition at the start of the coding sequence controls the balance between translation initiation and mRNA degradation in E. coli. [PDF]
Lipońska A +8 more
europepmc +1 more source
Thrombolytic proteins profiling: High‐throughput activity, selectivity, and resistance assays
We present optimized biochemical protocols for evaluating thrombolytic proteins, enabling rapid and robust screening of enzymatic activity, inhibition resistance, and fibrin affinity, stimulation, and selectivity. The outcome translates to key clinical indicators such as biological half‐life and bleeding risk. These assays streamline the development of
Martin Toul +3 more
wiley +1 more source
Efficiency of Digital Photolithographic Synthesis of Large, High-Quality DNA Libraries and Microarrays using a Guanine O<sup>6</sup> Dephosphitylation Strategy. [PDF]
Santhosh S +6 more
europepmc +1 more source
Overview of molecular signatures of senescence and associated resources: pros and cons
Cells can enter a stress response state termed cellular senescence that is involved in various diseases and aging. Detecting these cells is challenging due to the lack of universal biomarkers. This review presents the current state of senescence identification, from biomarkers to molecular signatures, compares tools and approaches, and highlights ...
Orestis A. Ntintas +6 more
wiley +1 more source
Double-stranded DNA deaminase DddA<sup>E1347A</sup> can increase the efficiency and targeting range of cytidine base editors. [PDF]
Qian Y +14 more
europepmc +1 more source
Structural and functional clues challenge the hypothesis that the yjdF riboswitch is natively regulated through broad recognition of azaaromatic compounds. [PDF]
Spradlin SF, Dickerson KA, Batey RT.
europepmc +1 more source
Exploiting nanopore sequencing advances for tRNA sequencing of human cancer models. [PDF]
Kochavi A +6 more
europepmc +1 more source
Long multiply marked DNA repair template reveals lengths and fidelity of genome editing tracts in <i>Schizosaccharomyces pombe</i>. [PDF]
Protacio RU +3 more
europepmc +1 more source

