Results 51 to 60 of about 10,826 (218)

The planar cell polarity protein Vangl2 interacts with the PDZ‐domains of Scribble but not with a unique PDZ‐like domain in Inturned

FEBS Letters, EarlyView.
Structural and biochemical characterisations show that the planar cell polarity (PCP) protein Inturned harbours a unique PDZ‐like domain that does not bind canonical PDZ‐binding motifs (PBMs) like that of another PCP protein Vangl2. In contrast, the apical‐basal polarity protein Scribble contains four PDZ domains that bind Vangl2, but one PDZ domain ...
Stephan Wilmes, Jan Brysch, Carmen Gelze, Lilli Meier, Daniel Kümmel   +4 more
wiley   +1 more source

Prediction of budburst, blooming and harvesting of apple trees using thermal sum models in mild winter conditions [PDF]

Brazilian Journal of Biology
Even though thermal sum models have become important tools to predict phenological stages and apples’ harvest, there have been many questions not only about which model to choose but also about their precision.
L. O. D. Marques, Í. R. Holz, M. S. Pasa, A. A. Sezerino, P. C. de Mello-Farias, J. L. Petri, F. G. Herter   +6 more
doaj   +1 more source

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Cardinal temperatures variability within a tropical japonica rice diversity panel

Plant Production Science, 2018
Air temperature is one of the most critical climatic factors controlling rice growth, development, and production in current and future climatic scenarii predicting increasingly frequent situations of extreme and/or fluctuating temperatures.
Lauriane Rouan, Alain Audebert, Delphine Luquet, Sandrine Roques, Audrey Dardou, Eric Gozé   +5 more
doaj   +1 more source

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

FEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa, Akira Hattori, Masafumi Tsujimoto, Toshihide Kobayashi   +3 more
wiley   +1 more source

Emissão de folhas e início de acumulação de amido em raízes de uma variedade de mandioca em função da época de plantio Leaf emergence and beginning of starch accumulation in roots of a cassava variety as a function of planting date

Ciência Rural, 2007
O número de folhas acumulado (NF) na haste principal está associado com o aparecimento de vários estádios de desenvolvimento da cultura da mandioca. O início de acumulação de amido (IAA) nas raízes tuberosas da mandioca marca o início da translocação dos
Alfredo Schons, Nereu Augusto Streck, Bruno Kraulich, Diego Garrido Pinheiro, Alencar Junior Zanon   +4 more
doaj   +1 more source

Analysis of Copernicus’ ERA5 Climate Reanalysis Data as a Replacement for Weather Station Temperature Measurements in Machine Learning Models for Olive Phenology Phase Prediction

Sensors, 2020
Knowledge of phenological events and their variability can help to determine final yield, plan management approach, tackle climate change, and model crop development.
Noelia Oses, Izar Azpiroz, Susanna Marchi, Diego Guidotti, Marco Quartulli, Igor G. Olaizola   +5 more
doaj   +1 more source