Development of Nanoflare Nanosensors for Monitoring Cellular Insulin mRNA in Beta Cells and Islets
Advanced Materials Technologies, EarlyView.This study presents the design and application of In2‐NF probes for detecting insulin mRNA in beta cells and islets. Using spherical nucleic acid‐coated gold nanoparticles, the probes offer strong fluorescent signals upon hybridization. Their performance is validated across 2D and 3D cultures and rat islets, demonstrating sensitive, real‐time mRNA ...
Yana Hu +7 more
wiley +1 more source
Advanced Materials Technologies, EarlyView.A mask‐free and cost‐effective UV‐pico‐second laser‐based microfabrication method is proposed to fabricate large‐area biodegradable microelectrode arrays and pressure sensors. These devices demonstrate low impedance, good conformability, excellent biocompatibility, and rapid degradation, providing a new route for next‐generation transient electronics ...
Bhavani Prasad Yalagala +5 more
wiley +1 more source
Amounts and species of probiotic lactic acid bacteria affect stimulation of short-chain fatty acid production in fecal batch culture. [PDF]
Biosci Microbiota Food Health, 2023 Ohashi Y, Fujisawa T.
europepmc +1 more source
Tunable 3D‐Printed Static Mixers for Gradient Bioprinting With High Cell Viability
Advanced Materials Technologies, EarlyView.ABSTRACT The fabrication of native tissue‐like structures with gradual transitions in material properties, cell types, and growth factors remains a major challenge in biofabrication due to the lack of suitable methods. Mimicking the hierarchical organization of living tissues is essential for functional models, yet creating gradient, multimaterial ...
Florian Hofmann +9 more
wiley +1 more source
Effect of low-level ultrasound treatment on the production of L-leucine by Corynebacterium glutamicum in fed-batch culture. [PDF]
Bioengineered, 2021 Zhang Y, Chen Z, Sun P, Xu Q, Chen N.
europepmc +1 more source
An Accessible Microfluidic Platform for the Generation of Polarized Brain Organoids
Advanced Materials Technologies, EarlyView.This study presents an accessible double‐gradient microfluidic device that enables controlled morphogen delivery and spatially polarized differentiation in brain organoids. By integrating a pump‐free design and stable fabrication method, the platform generates dorsal–ventral neuronal asymmetry, offering a reproducible and versatile approach to model ...
Daniel Pérez‐Calixto +6 more
wiley +1 more source
Production of thermostable pullulanase from Bacillus Flavothermus KWF-1 in fed-batch culture [PDF]
, 2011 Optimization of pullulanase production by Bacillus flavothermus KWF-1 in fed batch cultures was carried out using 2 L bioreactor with working volume of 1.5 L.
Ramanathan, Shankar
core
Removal of Toxic Volatile Compounds in Batch Culture Prolongs Stationary Phase and Delays Death of Escherichia coli. [PDF]
Appl Environ Microbiol, 2021 Osborne MG +4 more
europepmc +1 more source
Advanced Robotics Research, EarlyView.An AI‐powered, robot‐assisted framework automatically produces, images, and analyzes 3D tumor spheroids to evaluate drug efficacy. Integrated modules handle spheroid formation, live/dead staining, brightfield imaging, and automated image analysis, including spheroid segmentation, viability and metrics to assess the drug treatment efficacy. The workflow
Dalia Mahdy +13 more
wiley +1 more source
Investigating the influence of physiologically relevant hydrostatic pressure on CHO cell batch culture. [PDF]
Sci Rep, 2021 Shang M +5 more
europepmc +1 more source