Optimization of Culture Media and Feeding Strategy for High Titer Production of an Adenoviral Vector in HEK 293 Fed-Batch Culture [PDF]
VaccinesAdenoviruses are efficient and safe vectors for delivering target antigens and adenovirus-based vaccines have been used against a wide variety of pathogens, including tuberculosis and COVID-19.
Chun Fang Shen +4 more
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In vitro growth of Curcuma longa L. in response to five mineral elements and plant density in fed-batch culture systems. [PDF]
PLoS ONE, 2015 Plant density was varied with P, Ca, Mg, and KNO3 in a multifactor experiment to improve Curcuma longa L. micropropagation, biomass and microrhizome development in fed-batch liquid culture. The experiment had two paired D-optimal designs, testing sucrose
Rabia F El-Hawaz +2 more
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Double-stranded RNA production and the kinetics of recombinant Escherichia coli HT115 in fed-batch culture [PDF]
Biotechnology Reports, 2018 Double-stranded RNA can induce interference processes. The specificity of this system raises the possibility of using dsRNA for therapeutic applications targeting viral diseases. Escherichia coli HT115 (DE3) has been widely used to produce dsRNA; however,
Ljubomir Papić +3 more
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Progress in fed-batch culture for recombinant protein production in CHO cells [PDF]
Applied Microbiology and Biotechnology, 2023 Tian-Yun Wang, Wang Tian-Yun
exaly +2 more sources
Viable cell density as an indicator for dynamic feeding strategy in fed-batch and perfusion CHO cell culture [PDF]
Scientific ReportsThe general approach to industrial production of monoclonal antibodies is fed-batch culture using Chinese Hamster Ovary (CHO) cells. Perfusion culture is also attracting attention as a next-generation culture method.
Takuya Kikuchi +2 more
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Process-Driven Acetate-Based Lipid Production by the Oleaginous Yeast Lipomyces starkeyi [PDF]
MicroorganismsOleaginous yeasts are promising microbial platforms for lipid production from non-conventional carbon sources; however, acetate utilization is frequently constrained by physiological limitations associated with culture pH.
Akihiro Ishioka +5 more
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Optimization Feed Composition on Hyaluronic Acid Production of in-Batch and Fed-Batch Cultures of Streptococcus zooepidemicus [PDF]
Iranian Journal of Chemistry & Chemical Engineering, 2022 Hyaluronic acid (HA), as a high-value-added biological product, is one of the most widely used biopolymers in industry and medicine. This study aimed to increase HA production by carefully investigating the effect of glucose concentration, type and ...
Saeed Saharkhiz, Valiollah Babaeipour
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PLoS ONE, 2021 The yeast Saccharomyces cerevisiae is a reference model system and one of the widely used microorganisms in many biotechnological processes. In industrial yeast applications, combined strategies aim to maximize biomass/product yield, with the fed-batch ...
Emanuela Palomba +6 more
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Heterologous expression of Cysteine Protease 8 from Trichomonas foetus in Pichia pastoris [PDF]
Journal of Veterinary and Animal Sciences, 2023 Bovine trichomonosis is one of the most neglected venereal diseases of cattle. Trichomonas foetus, the causative organism was known over decades and is responsible for severe reproductive failure.
Karli Geethanjali +2 more
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Microbial Cell Factories, 2020 Background Docosahexaenoic acid (DHA) is essential for human diet. However, high production cost of DHA using C. cohnii makes it currently less competitive commercially, which is mainly caused by low DHA productivity.
Liangsen Liu +7 more
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