Optimization of Culture Media and Feeding Strategy for High Titer Production of an Adenoviral Vector in HEK 293 Fed-Batch Culture [PDF]
VaccinesAdenoviruses are efficient and safe vectors for delivering target antigens and adenovirus-based vaccines have been used against a wide variety of pathogens, including tuberculosis and COVID-19.
Chun Fang Shen +4 more
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Optimization of cyclosporin A production by Beauveria nivea in continuous fed-batch fermentation [PDF]
Archives of Biological Sciences, 2011 To develop the effective control method for fed-batch culture of cyclosporin A production, we chose fructose, L-valine and (NH4)2HPO4 as feeding nutrients and compared their productivities in relation to different concentrations.
Dong Huijun +3 more
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Double-stranded RNA production and the kinetics of recombinant Escherichia coli HT115 in fed-batch culture [PDF]
Biotechnology Reports, 2018 Double-stranded RNA can induce interference processes. The specificity of this system raises the possibility of using dsRNA for therapeutic applications targeting viral diseases. Escherichia coli HT115 (DE3) has been widely used to produce dsRNA; however,
Ljubomir Papić +3 more
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In vitro growth of Curcuma longa L. in response to five mineral elements and plant density in fed-batch culture systems. [PDF]
PLoS ONE, 2015 Plant density was varied with P, Ca, Mg, and KNO3 in a multifactor experiment to improve Curcuma longa L. micropropagation, biomass and microrhizome development in fed-batch liquid culture. The experiment had two paired D-optimal designs, testing sucrose
Rabia F El-Hawaz +2 more
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Viable cell density as an indicator for dynamic feeding strategy in fed-batch and perfusion CHO cell culture [PDF]
Scientific ReportsThe general approach to industrial production of monoclonal antibodies is fed-batch culture using Chinese Hamster Ovary (CHO) cells. Perfusion culture is also attracting attention as a next-generation culture method.
Takuya Kikuchi +2 more
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Process-Driven Acetate-Based Lipid Production by the Oleaginous Yeast Lipomyces starkeyi [PDF]
MicroorganismsOleaginous yeasts are promising microbial platforms for lipid production from non-conventional carbon sources; however, acetate utilization is frequently constrained by physiological limitations associated with culture pH.
Akihiro Ishioka +5 more
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Progress in fed-batch culture for recombinant protein production in CHO cells. [PDF]
Appl Microbiol Biotechnol, 2023 Xu WJ, Lin Y, Mi CL, Pang JY, Wang TY.
europepmc +2 more sources
Optimization Feed Composition on Hyaluronic Acid Production of in-Batch and Fed-Batch Cultures of Streptococcus zooepidemicus [PDF]
Iranian Journal of Chemistry & Chemical Engineering, 2022 Hyaluronic acid (HA), as a high-value-added biological product, is one of the most widely used biopolymers in industry and medicine. This study aimed to increase HA production by carefully investigating the effect of glucose concentration, type and ...
Saeed Saharkhiz, Valiollah Babaeipour
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Process development for high-titer production of adenovirus devoid of replication-competent particles in suspension-adapted complementing A549 cell culture [PDF]
BMC BiotechnologyAdenovirus is one of the most attractive viral vectors for therapeutic vaccines and gene therapy with the caveat that replication-competent adenoviruses (RCA) can be produced.
Chun Fang Shen +4 more
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PLoS ONE, 2021 The yeast Saccharomyces cerevisiae is a reference model system and one of the widely used microorganisms in many biotechnological processes. In industrial yeast applications, combined strategies aim to maximize biomass/product yield, with the fed-batch ...
Emanuela Palomba +6 more
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