Results 11 to 20 of about 53,182 (300)
Feed optimization in fed-batch culture
, 2014 Fed-batch processes are a current preference for the production of recombinant proteins in mammalian cells. The use of nutrient feeding prevents the depletion of important medium components, and results in improved culture longevity and high cell and ...
Henriques, Mariana +4 more
core +4 more sources
Enhanced production of recombinant streptokinase in Escherichia coli using fed-batch culture
Bioresource Technology, 2009 Fed-batch culture strategy is often used for increasing production of heterologous recombinant proteins in Escherichia coli. This study was initiated to investigate the effects of dissolved oxygen concentration (DOC), complex nitrogen sources and pH ...
Debendra K Sahoo
exaly +2 more sources
Efficient biosynthesis of exopolysaccharide in Candida glabrata by a fed-batch culture. [PDF]
Front Bioeng Biotechnol, 2022 Polysaccharides are important natural biomacromolecules. In particular, microbial exopolysaccharides have received much attention. They are produced by a variety of microorganisms, and they are widely used in the food, pharmaceutical, and chemical industries.
Xu S, Xu J, Zeng W, Shan X, Zhou J.
europepmc +4 more sources
Optimization Feed Composition on Hyaluronic Acid Production of in-Batch and Fed-Batch Cultures of Streptococcus zooepidemicus [PDF]
Iranian Journal of Chemistry & Chemical Engineering, 2022 Hyaluronic acid (HA), as a high-value-added biological product, is one of the most widely used biopolymers in industry and medicine. This study aimed to increase HA production by carefully investigating the effect of glucose concentration, type and ...
Saeed Saharkhiz, Valiollah Babaeipour
doaj +1 more source
PLoS ONE, 2021 The yeast Saccharomyces cerevisiae is a reference model system and one of the widely used microorganisms in many biotechnological processes. In industrial yeast applications, combined strategies aim to maximize biomass/product yield, with the fed-batch ...
Emanuela Palomba +6 more
doaj +2 more sources
Heterologous expression of Cysteine Protease 8 from Trichomonas foetus in Pichia pastoris [PDF]
Journal of Veterinary and Animal Sciences, 2023 Bovine trichomonosis is one of the most neglected venereal diseases of cattle. Trichomonas foetus, the causative organism was known over decades and is responsible for severe reproductive failure.
Karli Geethanjali +2 more
doaj +1 more source
Microbial Cell Factories, 2020 Background Docosahexaenoic acid (DHA) is essential for human diet. However, high production cost of DHA using C. cohnii makes it currently less competitive commercially, which is mainly caused by low DHA productivity.
Liangsen Liu +7 more
doaj +1 more source
A serum substitute for fed-batch culturing of hybridoma cells [PDF]
Cytotechnology, 2008 We developed a substitute for serum to produce fed-batch cultures of hybridoma cells in serum-free medium and confirmed that the cells could be successfully cultivated this way. Our substitute consisted of 12 components. The specific production rates of lactate and ammonia, which are harmful byproducts from the cells, were significantly reduced ...
Keisuke, Shibuya +2 more
openaire +2 more sources
AMB Express, 2022 Key points Trichoderma D-221704 produced 53 g L−1 mucic acid in fed batch culture at pH 4.0 Yeast extract was beneficial, but not essential for good mucic acid production The ambr®250 gave good, reproducible fed-batch cultivations with Trichoderma sp.
Anu Tamminen +4 more
doaj +1 more source
Fermentation, 2018 High initial glucose concentrations may inhibit glucose utilization and decrease ethanol fermentation efficiency. To minimize substrate inhibition, the effects of feeding yeast with different glucose concentrations on the ethanol production by batch and ...
Yi-Huang Chang +5 more
doaj +1 more source