Results 41 to 50 of about 53,182 (300)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

open access: yesFEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz   +6 more
wiley   +1 more source

Simulation of kefiran production of Lactobacillus kefiranofaciens JCM6985 in fed-batch reactor [PDF]

open access: yesSongklanakarin Journal of Science and Technology (SJST), 2006
Kinetics of kefiran production by Lactobacillus kefiranofaciens JCM6985 has been investigated. A mathematical model taking into account the mechanism of exopolysaccharides production has been developed. Experiments were carried out in batch mode in order
Benjamas Cheirsilp
doaj  

Acetamide degradation by a continuous-fed batch culture ofBacillus sphaericus

open access: yesApplied Biochemistry and Biotechnology, 1998
The methanogenesis of acetamide occurs through a two-step reaction in methanogenic sludges. First, acetamide is hydrolyzed to acetate and ammonia by a strict aerobic bacterium (Bacillus sphaericus), then acetate is used by Bacillus as carbon source or converted to methane by methanogens.
Ramirez, F.   +4 more
openaire   +3 more sources

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

open access: yesFEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen   +9 more
wiley   +1 more source

Production of ethanol by fed-batch fermentation [PDF]

open access: yes, 2009
The production of ethanol, from glucose in batch and fed batch culture, was investigated. In the fed batch culture, the glucose feeding was added into the culture at 16th hour of fermentation.
Abbase
core   +1 more source

Xylitol production by Candida parapsilosis under fed-batch culture

open access: yesBrazilian Archives of Biology and Technology, 2001
Xylitol production by Candida parapsilosis was investigated under fed-batch cultivation, using single (xylose) or mixed (xylose and glucose) sugars as substrates.
Sandra A. Furlan, Heizir F. de Castro
doaj   +1 more source

Developing a single-stage continuous process strategy for vitamin B12 production with Propionibacterium freudenreichii

open access: yesMicrobial Cell Factories, 2023
Background Vitamin B12 is a widely used compound in the feed and food, healthcare and medical industries that can only be produced by fermentation because of the complexity of its chemical synthesis. Besides, the use of Generally Recognized as Safe (GRAS)
Álvaro Calvillo   +3 more
doaj   +1 more source

Application of hypoosmolar medium to fed-batch culture of hybridoma cells for improvement of culture longevity

open access: yes, 1999
Cell culture longevity in fed-batch culture of hybridomas is often limited by elevated medium osmolality caused by repeated nutrient feeding. Shotwise feeding of 10x Dulbeccos modified Eagles medium (DMEM) concentrates elevated the osmolality of medium ...
Lee, Gyun Min, Ryu, J.S.
core   +1 more source

Derivation and characterization of retinal pigment epithelium from urine‐derived iPSCs

open access: yesFEBS Open Bio, EarlyView.
Age‐related macular degeneration causes vision loss via RPE dysfunction and loss. Traditional iPSC therapies rely on invasive biopsies, limiting scalability. Here, we utilize urine‐derived stem cells as an accessible source to generate u‐iPSCs, successfully differentiated into pigmented RPE. This “Urine‐to‐Retina” platform provides a promising path for
Daniella Beiner   +7 more
wiley   +1 more source

Modelling of a Fed-Batch Culture Applying Simulated Annealing

open access: yesBiomath, 2012
In this paper the metaheuristic Simulated Annealing (SA) is applied for parameter identification of non-linear model of cultivation process. SA algorithm is a stochastic relaxation technique, using the Metropolis algorithm based on the Boltzmann ...
Olympia Roeva, Tanya Trenkova
doaj   +1 more source

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