Results 61 to 70 of about 53,182 (300)

Metabolic Phenotyping of CHO Cells Varying in Cellular Biomass Accumulation and Maintenance during Fed-Batch Culture [PDF]

, 2017
CHO cell lines capable of high-level recombinant protein product biosynthesis during fed-batch culture are still generally obtained by intensive empirical screening of transfected cells rather than knowledge-guided cellular engineering. In this study, we
James, D.C., Alejandro Fernandez-Martell, Yusuf B. Johari, Johari, Y.B., Fernandez-Martell, A., David C. James   +5 more
core   +1 more source

Development of a Synthetic Hydrogel to Foster Microvascularization of an Endometriosis Microphysiological System

Advanced Healthcare Materials, EarlyView.
ABSTRACT The ascent of novel alternative methods in drug development spotlights the dual needs for improved biological fidelity to in vivo, along with reproducibility, especially in regulatory applications. The need for pre‐clinical models of patient‐derived endometriosis lesions motivates the development of a vascularizable, completely synthetic ...
Lauren Pruett, Laura Bahlmann, Ryan Ogi, Angela Jiao, Priyatanu Roy, Matthew Johnson, David Trumper, Linda Griffith   +7 more
wiley   +1 more source

Development of a simple and high-yielding fed-batch process for the production of porcine circovirus type 2 virus-like particle subunit vaccine

AMB Express, 2019
The cap protein is encoded by the orf2 gene of porcine circovirus type 2 (PCV2) has the main antigen epitope of PCV2 and can form virus-like particles (VLPs), which are expressed in insect cells.
Wenlong Cao, Hui Cao, Xiaoping Yi, Yingping Zhuang   +3 more
doaj   +1 more source

Respiratory Organ‐on‐a‐Chip for Disease Modeling: From Architecture to Functional Integration

Advanced Healthcare Materials, EarlyView.
Respiratory organ‐on‐a‐chip (ROC) models capture key mechanical and cellular cues of the human respiratory system, enabling quantitative dissection of disease mechanisms. This review links ROC architectures to disease modeling, functional integration, and commercialization, and proposes a decision framework that aligns model complexity with mechanistic
Jinzhuo Hu, Yongjie Tang, Sidi Liu, Tao Xu, Qian Liu   +4 more
wiley   +1 more source

Optimization of cyclosporin A production by Beauveria nivea in continuous fed-batch fermentation [PDF]

Archives of Biological Sciences, 2011
To develop the effective control method for fed-batch culture of cyclosporin A production, we chose fructose, L-valine and (NH4)2HPO4 as feeding nutrients and compared their productivities in relation to different concentrations.
Dong Huijun, Jiang Junyun, Yan Tongshun, Zhao Junjie   +3 more
doaj   +1 more source

Fed-batch fermentation of Tuber melanosporum for the hyperproduction of mycelia and bioactive Tuber polysaccharides

, 2009
For the first time, a fed-batch fermentation process of Tuber melanosporum was developed for the efficient production of bioactive mycelia and Tuber polysaccharides.
Zhong, Jian-Jiang, Li, Dong-Sheng, Liu, Qiao-Ning, Liu, Rui-Sang, Mi, Zhi-Yuan, Wang, Yi-Hua, Tang, Ya-Jie, Tang, YJ   +7 more
core   +1 more source

Microgel‐Based 3D Bioprinting: A Convergent Strategy Integrating Material Design, Jamming Dynamics, and Biological Function

Advanced Healthcare Materials, EarlyView.
Microgel‐based 3D printed constructs represent a compelling and versatile innovation for engineering architecturally complex, dynamically remodelable, and biocompatible structures with high structural fidelity and bioactivity. By integrating material design, biofabrication, and biological function, these systems enable the development of adaptive ...
Elena Ghighină, Andreea Ioana Dinu, Jana Ghițman   +2 more
wiley   +1 more source

Enhancing Biomass Production and Biodesulfurization Efficiency of Rhodococcus qingshengii IGTS8: Evaluation of Batch, Repeated Batch and Fed-Batch Cultivation Techniques

Applied Sciences
The batch, repeated batch and fed-batch cultivation strategies, in stirred tank bioreactors, were evaluated to maximize biomass production and the cells’ desulfurization activity (CDA) of Rhodococcus qingshengii IGTS8.
Konstantinos Dimos, Styliani Kalantzi, George Prasoulas, Panagiotis D. Gklekas, Olga Martzoukou, Dimitris G. Hatzinikolaou, Dimitris Kekos, Diomi Mamma   +7 more
doaj   +1 more source

Assembling a True “Olympic Gel” From over 16 000 Combinatorial DNA Rings

Advanced Materials, EarlyView.
Olympic gels are an elusive class of soft matter, consisting of molecular networks held together purely by mechanically interlocked rings. Their topological structure promises unique properties and functions, but their synthesis has proven notoriously difficult.
Sarah K. Speed, Yu‐Hsuan Peng, Azra Atabay, Krishna Gupta, Toni Müller, Carolin Fischer, Ilka M. Hermes, Jens‐Uwe Sommer, Michael Lang, Elisha Krieg   +9 more
wiley   +1 more source

Viral Infection‐Inspired Autonomous Detection of Fusion‐Competent Viruses for Screening and Environmental Surveillance

Advanced Materials, EarlyView.
Inspired by viral entry mechanisms, the FUSION assay enables autonomous detection of respiratory viruses via membrane fusion–triggered CRISPR‐Cas13a activation. VEACON selectively fuses with fusion‐competent viruses, triggering fluorescence within confined vesicles.
Jae Chul Park, Yidam Song, Hyun‐Woo Choi, Jaeuk Sung, Harin Jin, WonSeok Gwak, Kirim Yoo, Soohwang Lee, Jongeon Park, Jeongmin Kim, Hye‐Jun Jo, Jahyun Koo, Youngdo Jeong, Kwan Hyi Lee, Seung‐Jung Kee, Hojun Kim   +15 more
wiley   +1 more source