Results 61 to 70 of about 28,581 (261)

WT1‐Targeted Oral Bifidobacterium longum Vaccine Enhances Checkpoint Blockade Efficacy in Pancreatic Cancer

Advanced Science, EarlyView.
Oral WT1‐targeted Bifidobacterium longum vaccine promotes WT1‐associated cellular immunity and enhances anti‐PD‐1/anti‐CTLA‐4 efficacy in pancreatic cancer. ABSTRACT Pancreatic ductal adenocarcinoma (PDAC) remains largely unresponsive to immune checkpoint inhibitors (ICI) due to its profoundly immunosuppressive tumor microenvironment.
Taiki Yamazaki, Aori Minami, Misako Hirota, Mirei Hirano, Ryota Sato, Atsuki Honma, Hideto Ueki, Toshiro Shirakawa   +7 more
wiley   +1 more source

Engineering Microbial Particles for Next‐Generation Biomedical Platforms

Advanced Science, EarlyView.
Microbe‐derived particles (MDPs), which include extracellular vesicles, outer membrane vesicles, inclusion bodies, polysaccharide particles, and virus‐like particles, represent a rapidly expanding category of bioinspired nanomaterials. With their natural origin, intrinsic biocompatibility, and highly programmable functionality, MDPs serve as a ...
Yuting Li, Yunjie Lu, Ziyan Huang, Jianjiang Chen, Yanbin Liu, Lei Qin, Chao Wang, Yumin Wu   +7 more
wiley   +1 more source

Fed-batch enzymatic hydrolysis of alkaline organosolv-pretreated corn stover facilitating high concentrations and yields of fermentable sugars for microbial lipid production

Biotechnology for Biofuels, 2020
Background Lignocellulosic biomass has been commonly regarded as a potential feedstock for the production of biofuels and biochemicals. High sugar yields and the complete bioconversion of all the lignocellulosic sugars into valuable products are ...
Zhiwei Gong, Xuemin Wang, Wei Yuan, Yanan Wang, Wenting Zhou, Guanghui Wang, Yi Liu   +6 more
doaj   +1 more source

Materials‐Guided Gene‐Ionizable Lipid Nanoparticles to Reverse Iron‐Associated Immune Resistance in Renal Cancer

Advanced Science, EarlyView.
ABSTRACT Iron overload is a common metabolic disturbance in cancer and contributes to poor outcomes in renal cell carcinoma (RCC), yet its effects on the tumour immune microenvironment remain unclear. Here we identify a previously unrecognized immunosuppressive axis in which iron overload downregulates the palmitoyltransferase ZDHHC12 in CD8+ T cells ...
Xin Jin, Yulong Hong, Chengliang Yin, Wanyang Guo, Yaxuan Wang, Ruijiang Zeng, Ruilin Liu, Zexian Ding, Xinlin Liu, Shangqing Ren, Qiyang Liang, Yaohui Wang, Xu Zhang, João Conde, Yuan Li, Xin Ma, Liangyou Gu   +16 more
wiley   +1 more source

Parallel substrate supply and pH stabilization for optimal screening of E. coli with the membrane-based fed-batch shake flask

Microbial Cell Factories, 2018
Background Screening in the fed-batch operation mode is essential for biological cultivations facing challenges as oxygen limitation, osmotic inhibition, catabolite repression, substrate inhibition or overflow metabolism.
P. Philip, D. Kern, J. Goldmanns, F. Seiler, A. Schulte, T. Habicher, J. Büchs   +6 more
doaj   +1 more source

Production of nattokinase by batch and fed-batch culture of Bacillus subtilis

New Biotechnology, 2010
Nattokinase was produced by batch and fed-batch culture of Bacillus subtilis in flask and fermentor. Effect of supplementing complex media (peptone, yeast extract, or tryptone) was investigated on the production of nattokinase. In flask culture, the highest cell growth and nattokinase activity were obtained with 50 g/L of peptone supplementation.
Young-Han, Cho, Jae Yong, Song, Kyung Mi, Kim, Mi Kyoung, Kim, In Young, Lee, Sang Bum, Kim, Hyeon Shup, Kim, Nam Soo, Han, Bong Hee, Lee, Beom Soo, Kim   +9 more
openaire   +2 more sources

PhosSight: A Unified Deep Learning Framework Boosting and Accelerating Phosphoproteome Identification to Enable Biological Discoveries

Advanced Science, EarlyView.
PhosSight is a unified deep‐learning framework for phosphoproteome identification, featured by a phosphorylation‐aware detectability predictor. It improves identification sensitivity in DDA through deep re‐localization and rescoring, accelerates DIA searches by detectability‐guided spectral library pruning, and expands phosphoproteome coverage to ...
Ben Wang, Zhiyuan Cheng, Chengying She, Hongwei Zhao, Jiahui Zhang, Lin Lv, Zhihao Yan, Hongwen Zhu, Lizhuang Liu, Yan Fu, Xinpei Yi   +10 more
wiley   +1 more source

Optimization of the Process of Chinese Hamster Ovary (CHO) Cell Fed-Batch Culture to Stabilize Monoclonal Antibody Production and Overall Quality: Effect of pH Control Strategies

Fermentation
Monoclonal antibodies (mAbs) used in biomedical research and therapeutic applications are primarily produced by Chinese hamster ovary (CHO) cells via fed-batch culture.
Kexue Liang, Hongzhen Luo, Qi Li
doaj   +1 more source

Modelling and Regularity of Nonlinear Impulsive Switching Dynamical System in Fed-Batch Culture

Abstract and Applied Analysis, 2012
A hybrid system with state-based switchings is proposed to describe the fed-batch production of 1,3-propandiol from glycerol in our previous work. However, the on-off switching of alkali is too frequent, which greatly increases the computational cost of ...
Lei Wang
doaj   +1 more source

Engineered Optogenetic Circuits In Yeast with Self‐Sustained Outputs

Advanced Science, EarlyView.
Optogenetic quorum‐sensing (OptoQS) circuit enables Saccharomyces cerevisiae to record transient light stimulus with self‐sustained outputs. Panel a indicates the design of the OptoQS circuit based on G‐protein coupled receptor (GPCR) signaling cascade. Panel b indicates the mode of action of surrogate messenger‐mediated signal transmission at the cell
Cong Fan, Haofeng Chen, Yan Wang, Junyi Wang, Yueyang Chen, Yang Zhang, Jinting Guan, Jifeng Yuan   +7 more
wiley   +1 more source