Results 31 to 40 of about 28,581 (261)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

open access: yesFEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz   +6 more
wiley   +1 more source

Simulation of kefiran production of Lactobacillus kefiranofaciens JCM6985 in fed-batch reactor [PDF]

open access: yesSongklanakarin Journal of Science and Technology (SJST), 2006
Kinetics of kefiran production by Lactobacillus kefiranofaciens JCM6985 has been investigated. A mathematical model taking into account the mechanism of exopolysaccharides production has been developed. Experiments were carried out in batch mode in order
Benjamas Cheirsilp
doaj  

CONVERSION OF PINEAPPLE JUICE WASTE INTO LACTIC ACID IN BATCH AND FED – BATCH FERMENTATION SYSTEMS

open access: yesReaktor, 2008
Pineapple juice waste contains valuable components, which are mainly sucrose, glucose, and fructose. Recently, lactic acid has been considered to be an important raw material for the production of biodegradable lactide polymer.
Abdullah Mochamad Busairi
doaj   +1 more source

Developing a single-stage continuous process strategy for vitamin B12 production with Propionibacterium freudenreichii

open access: yesMicrobial Cell Factories, 2023
Background Vitamin B12 is a widely used compound in the feed and food, healthcare and medical industries that can only be produced by fermentation because of the complexity of its chemical synthesis. Besides, the use of Generally Recognized as Safe (GRAS)
Álvaro Calvillo   +3 more
doaj   +1 more source

Derivation and characterization of retinal pigment epithelium from urine‐derived iPSCs

open access: yesFEBS Open Bio, EarlyView.
Age‐related macular degeneration causes vision loss via RPE dysfunction and loss. Traditional iPSC therapies rely on invasive biopsies, limiting scalability. Here, we utilize urine‐derived stem cells as an accessible source to generate u‐iPSCs, successfully differentiated into pigmented RPE. This “Urine‐to‐Retina” platform provides a promising path for
Daniella Beiner   +7 more
wiley   +1 more source

Xylitol production by Candida parapsilosis under fed-batch culture

open access: yesBrazilian Archives of Biology and Technology, 2001
Xylitol production by Candida parapsilosis was investigated under fed-batch cultivation, using single (xylose) or mixed (xylose and glucose) sugars as substrates.
Sandra A. Furlan, Heizir F. de Castro
doaj   +1 more source

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

open access: yesFEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti   +5 more
wiley   +1 more source

Modelling of a Fed-Batch Culture Applying Simulated Annealing

open access: yesBiomath, 2012
In this paper the metaheuristic Simulated Annealing (SA) is applied for parameter identification of non-linear model of cultivation process. SA algorithm is a stochastic relaxation technique, using the Metropolis algorithm based on the Boltzmann ...
Olympia Roeva, Tanya Trenkova
doaj   +1 more source

Fed-batch production and characterization of polyhydroxybutyrate by Bacillus megaterium LVN01 from residual glycerol

open access: yesDyna, 2020
The operating conditions of polyhydroxybutyrate (PHB) production processes are among the factors that most influence yields. In this study, we evaluated PHB production synthesized by Bacillus megaterium LVN01.
Javier Ricardo Gómez Cardozo   +5 more
doaj   +1 more source

Glucose transport engineering allows mimicking fed-batch performance in batch mode and selection of superior producer strains

open access: yesMicrobial Cell Factories, 2022
Background Fed-batch mode is the standard culture technology for industrial bioprocesses. Nevertheless, most of the early-stage cell and process development is carried out in batch cultures, which can bias the initial selection of expression systems ...
Daniela Velazquez   +5 more
doaj   +1 more source

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