Results 271 to 280 of about 168,995 (306)
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Activity of fructanase in batch cultures of oral streptococci
Carbohydrate Research, 1983Several strains of oral streptococci produced fructanase when grown in the absence of D-fructan in a complex medium supplemented with D-glucose. The major part of the activity was extracellular, and only 1-5% was associated with the cells. Release of fructanase began early in the exponential phase and the enzyme was stable in the stationary phase for ...
G J, Walker +2 more
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Sporulation of Penicillium camemberti in Submerged Batch Culture
Systematic and Applied Microbiology, 1999Sporulation of Penicillium camemberti was studied in submerged batch fermentation. A defined medium was used with glucose and ammonium as C- and N-sources. Temperature was set to 25 degrees C at pH 5.6. Essential for submerged sporulation was the presence of calcium (14 mM) which was adsorbed to the cell walls in all sporulating strains and inhibited ...
W, Bockelmann +3 more
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Repeated hybridoma batch culture with cell recycle
Cytotechnology, 1993At the end of a hybridoma batch culture, the cells are usually discarded after separation from the culture broth. If, however, they are aseptically recycled into the reactor, the production process can be resumed simply by the addition of fresh medium. This cycle can then be repeated several times consecutively.
H D, Blasey, A R, Bernard
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Reproduction of batch growth curves in continuous culture
Archiv f�r Mikrobiologie, 1964The authors have experimentally verified the theoretically deduced relation for the concentration of bacteria in the individual vessels of a multistage continuous system arranged so as to enable—at suitably chosen retention times in the individual vessels—the study of even rapidly occuring changes in the bacterial culture. Moreover, such system permits
B, SIKYTA, J, SLEZAK
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An adaptive control algorithm for fed‐batch culture
Biotechnology and Bioengineering, 1984AbstractThe implementation of adaptive control for a fed‐batch culture in order to maximize the output of product based on a self‐adjusting model is discussed in the present work. Optimization methods were applied to the generalized mathematical model of a fed‐batch fermentation process to determine control algorithms that could be used for on‐line ...
J, Stanissis, D, Levisauskas
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2020
The cells used in cell culture processes are all derived from animals. Their genetic and epigenetic control circuits and their physiology have evolved to carry out the physiological functions of the tissue from which they were derived, not to sustain themselves and proliferate in in vitro culture.
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The cells used in cell culture processes are all derived from animals. Their genetic and epigenetic control circuits and their physiology have evolved to carry out the physiological functions of the tissue from which they were derived, not to sustain themselves and proliferate in in vitro culture.
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Production of ?-fructofuranosidase by Aspergillus japonicus in batch and fed-batch cultures
Biotechnology Letters, 1995A comparison of β-fructofuranosidase (FFase, EC 3.2.l.26) production by Aspergillus japonicus TIT-90076 in batch and fed-batch cultures was investigated in shaken flasks. Results showed that fed-batch cultivation of A. japonicus using intermittent sucrose supply produced more FFase than batch culture, and the maximal enzyme production was 910 units ml ...
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Kinetics of α-amylase production in a batch and a fed-batch culture ofBacillus subtilis
Folia Microbiologica, 1984Analysis of the kinetics of alpha-amylase production in a batch and a fed-batch culture of Bacillus subtilis made it possible to derive a kinetic model of the process describing mutual interactions between growth and production. The specific growth rate is limited by the concentration of both corn-steep liquor and starch.
M A, Baig, J, Pazlarová, J, Votruba
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Transcriptional profiling of batch and fed-batch protein-free 293-HEK cultures
Metabolic Engineering, 2007Dynamic nutrient feeding to control glutamine at low levels in protein-free fed-batch cultures of 293-human embryonic kidney (HEK) cells achieved cell concentrations of 6 x 10(6) cells/ml. This represented a 4-fold improvement in cell concentration compared to batch cultures.
Lee, Y.Y. +4 more
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Fed-batch and continuous culture
1990Abstract A variety of techniques has been developed for the submerged culture of microorganisms. Batch and fed-batch culture have been part of the art of fermentation since antiquity and are still used today for most industrial fermentations.
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