Results 51 to 60 of about 168,995 (306)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

open access: yesFEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point   +7 more
wiley   +1 more source

Exon 7 splicing of ERα predicts poor prognosis and increases phenotypic heterogeneity in luminal a subtype breast cancer

open access: yesFEBS Open Bio, EarlyView.
ERα splice variant ERα∆7 lacks the C‐terminus, and its expression may change phenotypes of breast cancers. Our results showed that ERα∆7 is found in the luminal A subtype, and elevated ERα∆7 levels are linked to improved cell survival with lower proliferation and migration.
Long Wai Tsui   +10 more
wiley   +1 more source

Exopolysaccharide production by Lactobacillus confusus TISTR 1498 using coconut water as an alternative carbon source: the effect of peptone, yeast extract and beef extract [PDF]

open access: yesSongklanakarin Journal of Science and Technology (SJST), 2011
Coconut water (CW) is a by-product of food industry and has little value in Thailand. It is usually discarded as a wasteinto the environment. Consequently, we developed a value added process of exopolysaccharide (EPS) production usingLactobacillus ...
Phisit Seesuriyachan   +2 more
doaj  

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

open access: yesFEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen   +9 more
wiley   +1 more source

A Real-Time Multiplexed Microbial Growth Intervalometer for Capturing High-Resolution Growth Curves

open access: yesFrontiers in Microbiology, 2019
Batch cultures are a low maintenance and routine culturing method in microbiology. Automated tools that measure growth curves from microorganisms grown in traditional laboratory glassware, such as Balch-type tubes, are not commercially available.
David C. Vuono   +5 more
doaj   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

open access: yesFEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane   +11 more
wiley   +1 more source

Produção de polihidroxialcanoatos por Cupriavidus necator, em batelada sequencial com reciclo externo de células, e modelagem do processo a partir da análise de fluxos metabólicos [PDF]

open access: yes, 2015
Tese (doutorado) - Universidade Federal de Santa Catarina, Centro Tecnológico, Programa de Pós-Graduação em Engenharia de Alimentos, Florianópolis, 2015.Os Polihidroxialcanoatos (PHAs), com destaque para o Poli(3-hidroxibutirato) (P(3HB)) e o Poli(3 ...
Schmidt, Mélodi
core  

Evaluating the effects of essential oils on methane production and fermentation under in vitro conditions

open access: yesItalian Journal of Animal Science, 2017
The effects of adding essential oils (EO) on rumen fermentation and methane production were examined. The aim of experiment one was to screen the effects of four different EO (clove oil (CLO), white thyme oil (WTO), citronella oil (CTO) and anise oil ...
Mevlüt Günal   +2 more
doaj   +1 more source

Blood‐based proteomic profiling reveals context‐dependent changes in BCL2‐associated signaling during taxane therapy in breast cancer patients

open access: yesFEBS Open Bio, EarlyView.
Chemotherapy side effects significantly impact cancer survivors' quality of life. Using protein levels in blood samples from breast cancer patients before and after 12 weeks of taxane treatment, we detected treatment‐dependent changes in calcium signaling and aging pathways associated with cancer recurrence.
Saira Munshani   +6 more
wiley   +1 more source

The Super-Spinner: a low cost animal cell culture bioreactor for the CO 2 incubator

open access: yes, 1994
Heidemann R, Riese U, Lütkemeyer D, Büntemeyer H, Lehmann J. The Super-Spinner: a low cost animal cell culture bioreactor for the CO 2 incubator. Cytotechnology.
Heidemann, R.   +4 more
core   +1 more source

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