Results 131 to 140 of about 34,280 (213)

Nicotinamide Reverses the Warburg Effect in CHO Cell Culture

open access: yesBiotechnology and Bioengineering, EarlyView.
Abstract The Warburg effect, the preferential conversion of glucose‐derived pyruvat to lactate despite available oxygen, is a key feature of Chinese hamster ovary (CHO) cell culture. Lactate accumulation in recombinant protein‐producing cell culture is an inefficient usage of glucose, as well as being deleterious to cells.
James Morrissey   +7 more
wiley   +1 more source

One‐Pot Synthesis of a Small Synthetic Ligand for Antibody Purification

open access: yesBiotechnology and Bioengineering, EarlyView.
One‐pot liquid‐phase synthesis of the synthetic ligand B1Al2A2 enables controllable ligand density and efficient capture of IgG and IgY with high recovery and purity, streamlining small‐ligand affinity purification workflows. ABSTRACT The development of synthetic affinity ligands offers a cost‐effective alternative to traditional biological ligands ...
Carolina Mota Natal   +8 more
wiley   +1 more source

Dynamic, Unconstrained Optimization of Secreted Enzyme Production in Fed‐Batch Fermentation Using Reinforcement Learning

open access: yesBiotechnology and Bioengineering, EarlyView.
ABSTRACT Reinforcement learning (RL) has been used to control a wide range of dynamic processes, especially ones that are too complex to model well or have stochastic environmental perturbations. Fed‐batch fermentations are subject to changes in starting cell growth rates and process variations that can affect cell growth and secreted target production.
Sai Harish Uthravalli   +3 more
wiley   +1 more source

Diffusion-driven fed-batch fermentation in perforated ring flasks. [PDF]

open access: yesBiotechnol Lett
Lüchtrath C   +5 more
europepmc   +1 more source

Metabolic Profiles of Carbohydrates in Streptococcus thermophilus During pH-Controlled Batch Fermentation. [PDF]

open access: yesFront Microbiol, 2020
Liu G   +8 more
europepmc   +1 more source

Real‐Time Biomass Estimation in High‐Density Yeast Fermentations Using Soft Sensor Modeling

open access: yesBiotechnology and Bioengineering, EarlyView.
Accurate biomass measurements, both offline and online, are essential to improve prediction and control in yeast fermentation. This study develops regression‐based predictive models to correlate offline measurements (Dry Cell Weight and OD600 from a spectrophotometer) with online OD860 probe signals to provide accurate real‐time biomass estimations and
Ana G. Del Hierro   +3 more
wiley   +1 more source

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