Results 21 to 30 of about 149 (147)

Molecular basis of isozyme formation of beta-galactosidases in Bacillus stearothermophilus: isolation of two beta-galactosidase genes, bgaA and bgaB [PDF]

Journal of Bacteriology, 1984
Bacillus stearothermophilus IAM11001 produced three beta-galactosidases, beta-galactosidase I, II, and III (beta-gal I, II, and III), which are detectable by polyacrylamide (nondenatured) gel electrophoresis. By connecting restriction fragments of the chromosomal DNA to plasmid vectors, followed by transformation of Escherichia coli, two beta ...
S Negoro, Hirosuke Okada, H Hirata
openaire   +3 more sources

Engineering the Future of Restorative Clinical Peripheral Nerve Surgery

Advanced Healthcare Materials, EarlyView.
What if damaged nerves could regenerate more effectively? This review unveils cutting‐edge strategies to restore nerve function, from biomaterial scaffolds and bioactive molecules to living engineered tissues. By accelerating axonal regrowth, preserving Schwann cells, and enhancing connectivity, these approaches are reshaping nerve repair—offering new ...
Justin C. Burrell, Zarina S. Ali, Eric L. Zager, Joseph M. Rosen, Mykhailo M. Tatarchuk, D. Kacy Cullen   +5 more
wiley   +1 more source

Concentrated beta-galactosidase and cell permeabilization from Saccharomyces fragilis IZ 275 for beta-galactosidase activity in the hydrolysis of lactose [PDF]

Food Science and Technology, 2019
Abstract The cheese whey shows an organic nutrient charge that can be used to obtain metabolites of interest by biotechnology of microorganisms. Thus, fermentative processes for enzyme production, in particular beta-galactosidase becomes feasible. The enzyme plays an important role in the biotech food industry to obtain milk and dairy products with low
Luiz Rodrigo Ito MORIOKA, Caroline dos Santos VIANA, Érika de Pádua ALVES, Fernanda Gonzales PAIÃO, Anderson Massaro TAKIHARA, Amanda Sayuri Senoo KAKUNO, Hélio Hiroshi SUGUIMOTO   +6 more
openaire   +5 more sources

RONIN/HCF1‐TFEB Axis Protects Against D‐Galactose‐Induced Cochlear Hair Cell Senescence Through Autophagy Activation

Advanced Science, EarlyView.
D‐galactose (D‐gal) induced inner ear hair cell senescence by inhibiting TFEB transcription. RONIN/HCF1 promotes TFEB transcription to prevent cochlear HCs from D‐gal‐induced senescence through autophagy activation. Abstract Age‐related hearing loss is characterized by senescent inner ear hair cells (HCs) and reduced autophagy.
Yongjie Wei, Yuhua Zhang, Wei Cao, Nan Cheng, Yun Xiao, Yongjun Zhu, Yan Xu, Lei Zhang, Lingna Guo, Jun Song, Su‐hua Sha, Buwei Shao, Fang Ma, Jingwen Yang, Zheng Ying, Zuhong He, Renjie Chai, Qiaojun Fang, Jianming Yang   +18 more
wiley   +1 more source

General Properties of Beta-Galactosidase of Xanthomonas campestris [PDF]

Applied and Environmental Microbiology, 1979
Partially purified β-galactosidase of Xanthomonas campestris required 32 to 37 C and pH 5.5 to 5.8 for optimum activity. The enzyme had low affinity for lactose hydrolysis ( K m = 22 mM) and was inhibited by thiol group reagents ...
Joseph F. Frank, George A. Somkuti
openaire   +3 more sources

Histone Lactylation Antagonizes Senescence and Skeletal Muscle Aging by Modulating Aging‐Related Pathways

Advanced Science, EarlyView.
This study reveals histone lactylation as a metabolic‐epigenetic regulator countering cellular senescence and skeletal muscle aging. Reduced lactylation impairs cell cycle, DNA repair and proteostasis pathways, while exercise restores glycolytic activity and lactylation levels, preserving muscle function.
Fanju Meng, Jianuo He, Xuebin Zhang, Wencong Lyu, Ran Wei, Shiyi Wang, Zhehao Du, Haochen Wang, Jinlong Bi, Xueyang Hua, Chao Zhang, Yiting Guan, Guoliang Lyu, Xiao‐Li Tian, Lijun Zhang, Wenbing Xie, Wei Tao   +16 more
wiley   +1 more source

Catch, Cut, or Block? Versatile 4‐N‐Derivatized Sialyl Glycosides for Influenza Virus Neuraminidase Detection and Purification

Angewandte Chemie, EarlyView.
Sialyl glycosides containing 4‐N‐derivatized sialic acids synthesized by a highly efficient one‐pot two‐enzyme (OP2E) chemoenzymatic sialylation strategy have been shown to be versatile probes. They can resist sialidase cleavage, be selective substrates by influenza sialidases, or be nanomolar substrate analog‐based inhibitors and affinity ligands ...
Yue Yuan, Hai Yu, Anand Kumar Agrahari, Jin Gao, Hyeog Kang, Robert Daniels, Xi Chen   +6 more
wiley   +2 more sources

Inhibition of Ferroptosis Delays Aging and Extends Healthspan Across Multiple Species

Advanced Science, EarlyView.
This study identifies ferroptosis as a key driver of cellular senescence, with its inhibition delaying aging and extending healthspan across species. During senescence, ferroptosis worsens, increasing oxidative stress and lipid peroxidation, which accelerate aging.
Hai‐Jun Fu, Xing‐Yue Zhou, Da‐Lian Qin, Qan Qiao, Qiao‐Zhi Wang, Shi‐Ying Li, Yun‐Fei Zhu, Ya‐Ping Li, Jiang‐Min Zhou, Hui Cai, Fei‐Hong Huang, Lu Yu, Long Wang, An‐Guo Wu, Jian‐Ming Wu, Xiao‐Gang Zhou   +15 more
wiley   +1 more source

Ethylene‐Activated E3 Ubiquitin Ligase MdEAEL1 Promotes Apple Fruit Softening by Facilitating the Dissociation of Transcriptional Repressor Complexes

Advanced Science, EarlyView.
Ethylene‐activated MdEAEL1 mediates the disassembly of the MdZFP3‐MdTPL4‐MdHDA19 transcriptional repression complex, upregulating the histone acetylation levels in the promoter regions of cell wall degradation‐related genes, consequently promoting softening during storage.
Tong Li, Li Liu, Guangxin Yang, Yingcong Cai, Yingda Wang, Bowen Sun, Le Sun, Weiting Liu, Aide Wang   +8 more
wiley   +1 more source

Thermostable Beta-Galactosidase from Fungi

Journal of Dairy Science, 1982
Abstract Forty-five strains of molds were screened for beta-galactosidase activity. Strains exhibiting enzyme activity were evaluated for cell yield as well as enzyme units available per liter of the medium, per gram cell dry weight, and per milligram protein in their cell free extracts.
H.M. Sonawat, Sanjeev Agrawal, S.M. Dutta   +2 more
openaire   +2 more sources