Results 61 to 70 of about 1,020,842 (327)

Immobilization of recombinant thermostable beta-galactosidase from Bacillus stearothermophilus for lactose hydrolysis in milk.

Journal of Dairy Science, 2009
A recombinant thermostable beta-galactosidase from Bacillus stearothermophilus was immobilized onto chitosan using Tris(hydroxymethyl)phosphine (THP) and glutaraldehyde, and a packed bed reactor was utilized to hydrolyze lactose in milk.
Wei Chen, Haiqin Chen, Yongjun Xia, Jiayu Yang, J. Zhao, F. Tian, H. Zhang, H. Zhang   +7 more
semanticscholar   +1 more source

Photothermal Hydrogel with Mn3O4 Nanoparticles Alleviates Intervertebral Disc Degeneration by Scavenging ROS and Regulating Extracellular Matrix Metabolism

Advanced Functional Materials, EarlyView.
The MPTT‐nanozyme‐hydrogel system (Mn3O4@ChS‐HA) provides a multifunctional therapeutic strategy for intervertebral disc degeneration (IVDD), effectively targeting oxidative stress and enhancing AF repair by restoring extracellular matrix (ECM) and redox homeostasis.
Yangyang Chen, Chan Xue, Feifei Ni, Binwu Hu, Miaoheng Yan, He Zhang, Yuxiang Hu, Xiaoyao Peng, Guangfang Li, Yu Han, Hongjian Liu, Zengwu Shao, Qin Wang, Yulong Wei   +13 more
wiley   +1 more source

Activated microglia release β−galactosidase that promotes inflammatory neurodegeneration

Frontiers in Aging Neuroscience
Beta (β)-galactosidase is a lysosomal enzyme that removes terminal galactose residues from glycolipids and glycoproteins. It is upregulated in, and used as a marker for, senescent cells.
Emily J. A. Kitchener, Jacob M. Dundee, Guy C. Brown   +2 more
doaj   +1 more source

Gene Expression, Activity and Localization of Beta-Galactosidases during Late Ripening and Postharvest Storage of Tomato Fruit

Agriculture, 2022
Beta-galactosidases (β-GALs) hold a key role in both fruit softening and the increase of total soluble solids during maturation. Despite determining both quality and potential postharvest longevity, β-GAL activity during ripening, with a special focus on
Dimitrios Fanourakis, Nikolaos Nikoloudakis, Konstantinos Paschalidis, Miltiadis V. Christopoulos, Eleni Goumenaki, Eleni Tsantili, Costas Delis, Georgios Tsaniklidis   +7 more
doaj   +1 more source

Nanostructured Protein Surfaces Inspired by Spider Silk

Advanced Materials, EarlyView.
Harnessing recombinant spider silk technology, bioengineered spidroin variants enable the creation of functionalized nanostructured coatings with tunable affinity for specific targets, supporting a broad range of applications ‐ from antifouling surfaces and targeted drug delivery to advanced cell therapies and precision bio‐patterning via lithography ...
Martin Humenik, Thomas Scheibel
wiley   +1 more source

Purification and characterization of an alpha-galactosidase from Aspergillus fumigatus

Brazilian Archives of Biology and Technology, 2005
Aspergillus fumigatus secreted invertase (beta-fructofuranosidase) and alpha-galactosidase enzymatic activities able to hydrolyzing raffinose oligosaccharides (RO).
Sebastião Tavares de Rezende, Valéria Monteze Guimarães, Marília de Castro Rodrigues, Carlos Roberto Felix   +3 more
doaj   +1 more source

Digital Bioassays on the Slipchip Microfluidic Devices

Advanced Sensor Research, EarlyView.
This review summarizes recent advances in SlipChip‐based digital bioassays, highlighting their simple “load and slip” operation for fluid partitioning which eliminate the need for sophisticated fluidic systems. Applications include digital nucleic acid analysis, digital protein analysis and single‐cell analysis, demonstrating the platform‘s potential ...
Qingqing Luo, Ziqing Yu, Weiyuan Lyu, Yang Luo, Lei Xu, Feng Shen   +5 more
wiley   +1 more source

TNF receptor–related factor 3 inactivation promotes the development of intrahepatic cholangiocarcinoma through NF‐κB‐inducing kinase–mediated hepatocyte transdifferentiation

Hepatology, EarlyView., 2022
Abstract Background and Aims Intrahepatic cholangiocarcinoma (ICC) is a deadly but poorly understood disease, and its treatment options are very limited. The aim of this study was to identify the molecular drivers of ICC and search for therapeutic targets.
Yuto Shiode, Takahiro Kodama, Satoshi Shigeno, Kazuhiro Murai, Satoshi Tanaka, Justin Y. Newberg, Jumpei Kondo, Shogo Kobayashi, Ryoko Yamada, Hayato Hikita, Ryotaro Sakamori, Hiroshi Suemizu, Tomohide Tatsumi, Hidetoshi Eguchi, Nancy A. Jenkins, Neal G. Copeland, Tetsuo Takehara   +16 more
wiley   +1 more source

Tryptophan Metabolite Indole‐3‐Aldehyde Induces AhR and c‐MYC Degradation to Promote Tumor Immunogenicity

Advanced Science, EarlyView.
Tryptophan metabolite Indole‐3‐aldehyde (I3A) downregulates cytosolic AhR and p‐ERK/c‐MYC proteins, together enhancing STAT3 phosphorylation and tumor immunogenicity. Overexpression of the Trp metabolic enzyme interleukin‐4‐induced gene‐1 (IL4I1) in tumor cells increases intracellular levels of I3A and enhances tumor immunogenicity.
Lei Cui, Zining Wang, Zixuan Guo, Hongxia Zhang, Yongxiang Liu, Huanling Zhang, Huan Jin, Feifei Xu, Xiaojuan Wang, Chunyuan Xie, Hui Guo, Tiantian Wang, Yanxun Lin, Qi Zhao, Penghui Zhou, Jing Tan, Jin‐Xin Bei, Peng Huang, Jinyun Liu, Xiaojun Xia   +19 more
wiley   +1 more source

IL‐31 levels correlate with pruritus in patients with cholestatic and metabolic liver diseases and is farnesoid X receptor responsive in NASH

Hepatology, EarlyView., 2022
IL‐31 levels correlate with pruritus in patients with cholestatic and metabolic liver diseases Abstract Background and Aims Pruritus is associated with multiple liver diseases, particularly those with cholestasis, but the mechanism remains incompletely understood.
Jun Xu, Ya Wang, Mina Khoshdeli, Matt Peach, Jen‐Chieh Chuang, Julie Lin, Wen‐Wei Tsai, Sangeetha Mahadevan, Wesley Minto, Lauri Diehl, Ruchi Gupta, Michael Trauner, Keyur Patel, Mazen Noureddin, Kris V. Kowdley, Aliya Gulamhusein, Christopher L. Bowlus, Ryan S. Huss, Robert P. Myers, Chuhan Chung, Andrew N. Billin   +20 more
wiley   +1 more source