Activated microglia release β−galactosidase that promotes inflammatory neurodegeneration
Frontiers in Aging NeuroscienceBeta (β)-galactosidase is a lysosomal enzyme that removes terminal galactose residues from glycolipids and glycoproteins. It is upregulated in, and used as a marker for, senescent cells.
Emily J. A. Kitchener +2 more
doaj +1 more source
Microfluidic detection and analysis by integration of evanescent wave sensing with thermocapillary actuation [PDF]
, 2005 An integrated system capable of microfluidic actuation, detection and sensing is described which combines evanescent wave sensing with thermocapillary manipulation.
Troian, S. M. +2 more
core
Pre‐Encoded IFN‐I Sensitivity Exacerbates Memory T Cell Senescence in Solid Tumors
Advanced Science, EarlyView.Type I interferon (IFN‐I) signaling promotes p21‐dependent cell cycle arrest in senescent tumor‐specific memory T cells, resulting in poor proliferative responses and solid tumor regression during cancer vaccination. Conversely, IFNα/β receptor blockade reinvigorates T cell proliferation to regress solid tumors and is more effective with increasing ...
Andrew Nguyen +4 more
wiley +1 more source
Purification and characterization of an alpha-galactosidase from Aspergillus fumigatus
Brazilian Archives of Biology and Technology, 2005 Aspergillus fumigatus secreted invertase (beta-fructofuranosidase) and alpha-galactosidase enzymatic activities able to hydrolyzing raffinose oligosaccharides (RO).
Sebastião Tavares de Rezende +3 more
doaj +1 more source
Xwnt-5A: a maternal Wnt that affects morphogenetic movements after overexpression in embryos of Xenopus laevis [PDF]
, 1993 To contribute to an understanding of the roles and mechanisms of action of Wnts in early vertebrate development, we have characterized the normal expression of Xenopus laevis Wnt-5A, and investigated the consequences of misexpression of this putative ...
Campbell, Robert M. +5 more
core
An integrated native mass spectrometry and top-down proteomics method that connects sequence to structure and function of macromolecular complexes. [PDF]
, 2018 Mass spectrometry (MS) has become a crucial technique for the analysis of protein complexes. Native MS has traditionally examined protein subunit arrangements, while proteomics MS has focused on sequence identification.
Campuzano, Iain DG +4 more
core +1 more source
Detection of Protein–Protein Interactions in Escherichia coli With Single Molecule Sensitivity
Advanced Science, EarlyView.This article describes a regulatory circuit in Escherichia coli able to detect protein–protein interactions with exquisite sensitivity. The interaction between two hybrid proteins fused to Bordetella pertussis adenylate cyclase and its activator calmodulin triggers a potent cyclic AMP signaling cascade.
Marilyne Davi, Daniel Ladant
wiley +1 more source
Study of β-Galactosidase Enzyme Activity Produced by Lactobacilli in Milk and Cheese
Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum, 2012 <p>Background and objective Lactose intolerance is a discomfort state that occurs in some people after ingestion of milk and it is due to insufficient amount of beta galactosidase in the human gut to digest lactose.
J Nowroozi
doaj
Advanced Science, EarlyView.Chronic septic arthritis involves intracellular bacterial persistence and lipid‐immune crosstalk via the PGRN‐BMP lysosomal axis. A dual‐targeting nanoparticle system (NPs@PGRN) restores lysosomal bactericidal function, reduces bacterial burden, and reprograms macrophage immunity, offering a novel therapeutic strategy. ABSTRACT Chronic septic arthritis,
Congsun Li +12 more
wiley +1 more source
Metagenome-assembled genome sequences of three uncultured planktomarina sp. strains from the Northeast Atlantic Ocean [PDF]
, 2020 We report three metagenome-assembled genomes (MAGs) of Planktomarina strains from coastal seawater (Portugal) to help illuminate the functions of understudied Rhodobacteraceae bacteria in marine environments.
Borges, Nuno +6 more
core +1 more source