Journal of Biological Chemistry, 1983 The two neutral beta-N-acetylhexosaminidases of rat brain have been purified by procedures involving extraction, concanavalin A-Sepharose, ammonium sulfate precipitation, DEAE-cellulose, hydroxyapatite, Sepharose 4B, and an affinity chromatography with 2-acetamido-N-(epsilon-aminocaproyl)-2-deoxy-beta-galactopyranosylamine bound to Sepharose.
T, Izumi, K, Suzuki
exaly +3 more sources
Transcriptomic insights into the interplay between polyketide biosynthesis and other secondary metabolite biosynthetic clusters and biological pathways in entomopathogen <i>Beauveria bassiana</i>. [PDF]
Front MicrobiolBackground and aimsThe polyketide synthase gene pks15 plays a critical role in insect virulence and cell wall formation in the entomopathogenic fungus Beauveria bassiana.
Toopaang W +8 more
europepmc +3 more sources
Cracking the “Sugar Code”: A Snapshot of N- and O-Glycosylation Pathways and Functions in Plants Cells [PDF]
, 2021 Glycosylation is a fundamental co-translational and/or post-translational modification process where an attachment of sugars onto either proteins or lipids can alter their biological function, subcellular location and modulate the development and ...
Bacic, Antony +7 more
core +2 more sources
Protein Glycosylation in Bryophytes Differs Subtly from That in Vascular Plants [PDF]
, 2022 Glycosylation substantially contributes to the physicochemical properties of proteins, and hence also cell walls. Moreover, they are key factors for the recognition of free or cell-bound glycoproteins by internal and external interaction partners.
Altmann, Friedrich, Stenitzer, David
core +2 more sources
Structural and functional insight into human O-GlcNAcase [PDF]
, 2017 O-GlcNAc hydrolase (OGA) removes O-linked N-acetylglucosamine (O-GlcNAc) from a myriad of nucleocytoplasmic proteins. Through co-expression and assembly of OGA fragments, we determined the three-dimensional structure of human OGA, revealing an unusual ...
Roth, Christian +9 more
core +6 more sources
Vacuolar targeting of recombinant antibodies in Nicotiana benthamiana [PDF]
, 2020 Plant-based platforms are extensively used for the expression of recombinant proteins, including monoclonal antibodies. However, to harness the approach effectively and leverage it to its full potential, a better understanding of intracellular processes ...
Lareu, Jorge Fabricio +6 more
core +1 more source
Sweet modifications modulate plant development [PDF]
, 2021 Plant development represents a continuous process in which the plant undergoes morphological, (epi)genetic and metabolic changes. Starting from pollination, seed maturation and germination, the plant continues to grow and develops specialized organs to ...
De Coninck, Tibo +4 more
core +2 more sources
Purification and characterization of an extracellular beta-n-acetylhexosaminidase from Paecilomyces persicinus [PDF]
Journal of Bacteriology, 1979 Both beta-N-acetylglucosaminidase nad beta-N-acetylgalactosaminidase activities were detected in the culture fluids of Paecilomyces persicinus P-10 after growth in a soybean meal-corn meal medium. The active material was purified by means of protamine sulfate fractionation and ultrafiltration, followed by ion exchange and gel chromatography.
L A, Eriquez, M A, Pisano
openaire +2 more sources
Prediction of Human Gut Bacterial Secreted Proteins and their Interactions with Human Colonic Proteins [PDF]
, 2023 The gut microbiome is known to interact with the human host and deviations from a healthy gut microbiome can have serious repercussions for host health, but our knowledge of bacterial mediators of these interactions lags behind our ability to identify ...
Velez-Cortes, Florencia
core +2 more sources
In vitro Validation of Chimeric β-Galactosylceramidase Enzymes With Improved Enzymatic Activity and Increased Secretion [PDF]
, 2020 Globoid Cell Leukodystrophy (GLD) is a lysosomal storage disease (LSD) caused by inherited defects of the β-galactosylceramidase (GALC) gene. The infantile forms display a rapid and aggressive central and peripheral nervous system (CNS and PNS ...
Cascino F +4 more
core +1 more source