Results 11 to 20 of about 14,075,605 (174)

Beta-N-acetylhexosaminidases HEXO1 and HEXO3 are responsible for the formation of paucimannosidic N-glycans in Arabidopsis thaliana. [PDF]

J Biol Chem, 2011
Most plant glycoproteins contain substantial amounts of paucimannosidic N-glycans instead of their direct biosynthetic precursors, complex N-glycans with terminal N-acetylglucosamine residues.
Liebminger E, Veit C, Pabst M, Batoux M, Zipfel C, Altmann F, Mach L, Strasser R.   +7 more
europepmc   +2 more sources

Isolation, Specificity, and Application of β-N-Acetylhexosaminidases from Penicillium crustosum

, 2023
β-N-acetylhexosaminidases have great potential in applied biocatalysis owing to their ability to act on a wide range of natural and modified substrates.
Helena Hronská, Michal Rosenberg, Vladimír Štefuca, Ema Ondrejková, Mária Bláhová   +4 more
core   +1 more source

β-N-Acetylhexosaminidases for Carbohydrate Synthesis via Trans-Glycosylation

, 2020
β-N-acetylhexosaminidases (EC 3.2.1.52) are retaining hydrolases of glycoside hydrolase family 20 (GH20). These enzymes catalyze hydrolysis of terminal, non-reducing N-acetylhexosamine residues, notably N-acetylglucosamine or N-acetylgalactosamine, in N ...
Vuillemin, Marlene, Marlene Vuillemin, Muschiol, Jan, Zeuner, Birgitte, Birgitte Zeuner, Meyer, Anne S., Anne S. Meyer, Jan Muschiol   +7 more
core   +1 more source

Purification of Advanced Therapeutics by Flow‐Through Pseudo‐Affinity Chromatography: Bi‐Specific mABs, Fc Fusion Proteins, and Adeno‐Associated Viruses

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Modern biopharmaceutical manufacturing requires purification platforms capable of processing structurally and functionally diverse products while addressing the challenge of removing persistent and high‐risk host cell proteins (HCPs).
Wenning Chu, Eduardo Barbieri, Arianna Minzoni, Shriarjun Shastry, Jacob P. LeBarre, Qingbo Shu, Taufika Williams, Xuanwen Li, Divya Chandra, Xuankuo Xu, Jerome Bill, David Roush, Joseph Taris, Patrick Gilbert, Andre Kiesewetter, Oliver Rammo, Michael Schulte, Christopher Major, Trina Mouchahoir, Steven Cramer, Cristiana Boi, Ruben G. Carbonell, Abraham Lenhoff, Stefano Menegatti   +23 more
wiley   +1 more source

Optimized Fertilization Balances Microbial Carbon and Nitrogen Use Efficiency: Evidence From Microbial Communities

Land Degradation &Development, EarlyView.
ABSTRACT Optimized fertilization involving reduced mineral N input and organic substitution can alter resource stoichiometry, microbial carbon use efficiency (CUE) and nitrogen use efficiency (NUE). However, how fertilization‐induced resource imbalance reshapes microbial community to regulate CUE and NUE remains unclear. We conducted a field experiment
Yazhen Chen, Yichen Xu, Haobo Xu, Juan Chen, Xiaozhou Huang, Wenqiong Jiang, Jinsong He, Xiaohong Wu, Xinxing He, Jun Wang, Xiaocui Liang, Wende Yan   +11 more
wiley   +1 more source

Backbone structures in human milk oligosaccharides: trans-glycosylation by metagenomic β-N-acetylhexosaminidases

, 2015
This paper describes the discovery and characterization of two novel β-N-acetylhexosaminidases HEX1 and HEX2, capable of catalyzing the synthesis of human milk oligosaccharides (HMO) backbone structures with fair yields using chitin oligomers as β-N ...
Lezyk, Mateusz Jakub, Zeuner, Birgitte, Meyer, A.S., Meyer, Anne S., Difilippo, E., Logtenberg, M. J., Zeuner, B., Logtenberg, M.J., Schols, H.A., Difilippo, Elisabetta, Mikkelsen, Jørn Dalgaard, Mikkelsen, J.D., Nyffenegger, Christian, Schols, H. A., Lezyk, M., Nordvang, Rune Thorbjørn, Nyffenegger, C., Nordvang, R.T.   +17 more
core   +1 more source

Functional and transcriptomic analyses in Neurospora crassa reveal a crucial role for the N‐glycoprotein deglycosylation process in fungal homeostasis

The FEBS Journal, EarlyView.
In Neurospora crassa, a cytosolic GH18 endo‐β‐n‐acetylglucosaminidase (ENGase) functions as an active deglycosylating enzyme. Deletion of this severely alters tolerance to multiple stress conditions and causes extensive transcriptomic reprogramming, including upregulation of a GH20 exo‐β‐n‐acetylhexosaminidase under ER stress, indicating that N. crassa
Anastasios Samaras, Tanim Jabid Hossain, Magnus Karlsson, Georgios Tzelepis   +3 more
wiley   +1 more source

Exploring Diverse Glycone Preferences of Aspergillus rutinosidases

Advanced Synthesis &Catalysis, Volume 368, Issue 11, 3 June 2026.
Rutinosidases from Aspergillus niger (AnRut) and A. oryzae (AoRut) catalyze hydrolysis and transglycosylation with non‐natural glycosides as donors, enabling glycosylation of alcoholic and phenolic acceptors. AnRut exhibits broad glycone promiscuity, while AoRut has a narrower substrate scope.
Jitka Brouzdová, Lucie Petrásková, Katerina Brodsky, Natalia Kulik, Helena Pelantová, Martina Hurtová, Andrea Vopálenská, Kristýna Slámová, Pavla Bojarová, Vladimír Křen   +9 more
wiley   +1 more source

Design and synthesis of naphthalimide group-bearing thioglycosides as novel β-N-acetylhexosaminidases inhibitors

, 2018
GH20 human β-N-acetylhexosaminidases (hsHex) and GH84 human O-GlcNAcase (hOGA) are involved in numerous pathological processes and emerged as promising targets for drug discovery. Based on the catalytic mechanism and structure of the catalytic domains of
Qing Yang, Huizhe Lu, Jianjun Zhang, Shengqiang Shen, Lili Dong, Wei Chen   +5 more
core   +1 more source

Plant Genetic Engineering: Technological Pathways, Application Scenarios, and Future Directions

Advanced Science, Volume 13, Issue 30, 28 May 2026.
This review maps the fast‐evolving landscape of plant genetic engineering, linking enabling platforms with trait‐focused applications in architecture optimization, stress resilience, yield improvement, and quality enhancement. It highlights how genome editing, transgenic strategies, and emerging multi‐gene approaches reshape breeding pipelines, while ...
Peilin Wang, Wenzhi Wang, Dongjiao Wang, Qibin Wu, Youxiong Que   +4 more
wiley   +1 more source