Methodology of generation and purification of anti-beta 2 glycoprotein I antibodies [PDF]
MethodsX, 2019 In this Method Article we are showing the methodology for generation and purification of Anti-Beta 2 Glycoprotein I (β2GPI) antibodies. First β2GPI was purified from human plasma, and recognized by Western Blot and anti-β2GPI antibodies of serum from ...
Marcelo Alarcon +4 more
doaj +6 more sources
beta 2-glycoprotein I and anti-beta 2-glycoprotein I antibodies: Where are we now? [PDF]
Rheumatology, 1997 beta 2-Glycoprotein I (beta 2-GPI), a plasma protein with in vitro anticoagulant properties, has been recognized to have an important role in the antiphospholipid syndrome (APS) as a cofactor and an (co)antigen in ELISA assays. Although beta 2-GPI levels
ISENBERG, DA +2 more
core +3 more sources
Immune Complexes of Beta-2-Glycoprotein I and IgA Antiphospholipid Antibodies Identify Patients With Elevated Risk of Thrombosis and Early Mortality After Heart Transplantation [PDF]
Frontiers in Immunology, 2019 Background: The presence of anti-Beta 2 glycoprotein antibodies (aB2GP1) of IgA isotype is common in patients with functional impairment of the organs in which B2GP1 is elaborated.
Manuel Serrano +9 more
doaj +3 more sources
Renal Transplantation Dramatically Reduces IgA Anti-beta-2-glycoprotein I Antibodies in Patients with Endstage Renal Disease [PDF]
Journal of Immunology Research, 2014 IgA anti-beta-2-glycoprotein I (aB2GPI) antibodies have been related to vascular pathology in the general population and mainly in hemodialyzed patients (prevalence 33%) in whom an elevated incidence of thrombosis and mortality is found. In this paper we
Manuel Serrano +9 more
doaj +4 more sources
Posttranslational forms of beta 2-glycoprotein I in the pathogenesis of the antiphospholipid syndrome. [PDF]
Thromb J, 2016 The antiphospholipid syndrome (APS) is an autoimmune disease characterised by a procoagulant state that predisposes to recurrent thrombosis and miscarriages. Two major discoveries have advanced our understanding of the underlying complex pathogenesis of the APS. The first was the discovery that beta-2 glycoprotein-1 (β2GPI) is the major auto antigen in
El-Assaad F +2 more
europepmc +6 more sources
In vivo modulation of angiogenesis by beta 2 glycoprotein I [PDF]
Journal of Autoimmunity, 2010 Beta 2 glycoprotein I (β2GPI) is the major auto antigen in the antiphospholipid syndrome but also interacts with fibrinolytic and angiogenic proteins. The aim of this study was to examine the angiogenic potential of β2GPI in vivo in β2GPI deficient mice utilizing angiogenic assays. β2GPI deficient mice show increased microvessel formation in comparison
Freda H Passam, S A Krilis
exaly +6 more sources
Pretransplant IgA-Anti-Beta 2 Glycoprotein I Antibodies As a Predictor of Early Graft Thrombosis after Renal Transplantation in the Clinical Practice: A Multicenter and Prospective Study. [PDF]
Front Immunol, 2018 Background Graft thrombosis is a devastating complication after renal transplantation. We recently described the association of anti-beta-2-glycoprotein-I (IgA-ab2GP1) antibodies with early graft loss mainly caused by thrombosis in a monocenter study ...
Morales JM +14 more
europepmc +4 more sources
Anticardiolipin antibodies recognize beta 2-glycoprotein I structure altered by interacting with an oxygen modified solid phase surface. [PDF]
Journal of Experimental Medicine, 1994 Anticardiolipin antibodies (aCL) derived from the sera of individuals exhibiting the antiphospholipid syndrome (APS) directly bind to beta 2- glycoprotein I (beta 2-GPI), which is adsorbed to an oxidized polystyrene surface.
Eiji Matsuura, D A Triplett, T Koike
exaly +3 more sources
Interaction of the antiphospholipid syndrome autoantigen beta-2 glycoprotein I with DNA and neutrophil extracellular traps. [PDF]
Clin Immunol, 2023 Beta-2 glycoprotein I (β2GPI) is a phospholipid-binding plasma protein and prominent autoantigen in antiphospholipid syndrome (APS). Here, we tested the hypothesis that β2GPI might bind to not only phospholipids, but also cell-free DNA and neutrophil ...
Kmeťová K +13 more
europepmc +2 more sources
Lysine residues control the conformational dynamics of beta 2-glycoprotein I [PDF]
Physical Chemistry Chemical Physics, 2018 We report on a strategy to permanently open up the closed conformation of soluble blood protein beta 2-glycoprotein I by chemical acetylation of lysine residues.
Ina Buchholz +3 more
semanticscholar +6 more sources