Methodology of generation and purification of anti-beta 2 glycoprotein I antibodies [PDF]
MethodsX, 2019 In this Method Article we are showing the methodology for generation and purification of Anti-Beta 2 Glycoprotein I (β2GPI) antibodies. First β2GPI was purified from human plasma, and recognized by Western Blot and anti-β2GPI antibodies of serum from ...
Marcelo Alarcon +4 more
doaj +4 more sources
beta 2-Glycoprotein I and anti-beta 2-glycoprotein I antibodies: where are we now? [PDF]
Rheumatology, 1997 beta 2-Glycoprotein I (beta 2-GPI), a plasma protein with in vitro anticoagulant properties, has been recognized to have an important role in the antiphospholipid syndrome (APS) as a cofactor and an (co)antigen in ELISA assays. Although beta 2-GPI levels were found to be increased in some patients with APS, the clinical value of measuring beta 2-GPI ...
ISENBERG, DA +2 more
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Beta‐2‐Glycoprotein‐I Deficiency Could Precipitate an Antiphospholipid Syndrome‐like Prothrombotic Situation in Patients With Coronavirus Disease 2019 [PDF]
ACR Open Rheumatology, 2021 Objective Patients with coronavirus disease 2019 (COVID‐19) present coagulation abnormalities and thromboembolic events that resemble antiphospholipid syndrome (APS).
Manuel Serrano +17 more
doaj +2 more sources
B cell-derived anti-beta 2 glycoprotein I antibody mediates hyperhomocysteinemia-aggravated hypertensive glomerular lesions by triggering ferroptosis [PDF]
Signal Transduction and Targeted Therapy, 2023 Hyperhomocysteinemia (HHcy) is a risk factor for chronic kidney diseases (CKDs) that affects about 85% CKD patients. HHcy stimulates B cells to secrete pathological antibodies, although it is unknown whether this pathway mediates kidney injury.
Xing Du +16 more
doaj +2 more sources
Specific domain V reduction of beta-2-glycoprotein I induces protein flexibility and alters pathogenic antibody binding [PDF]
Scientific Reports, 2021 Beta-2-glycoprotein I (β2GPI) is a blood protein and the major antigen in the autoimmune disorder antiphospholipid syndrome (APS). β2GPI exists mainly in closed or open conformations and comprises of 11 disulfides distributed across five domains.
Ina Buchholz +11 more
doaj +2 more sources
Beyond antibodies: Beta-2 glycoprotein I as the unsung guardian of pregnancy. [PDF]
PLoS ONEThe physiological function of beta 2 glycoprotein I (β2GPI) itself is not well understood, other than that it is a primary antigen to anti-phospholipid antibodies in the autoimmune disease antiphospholipid syndrome.
Kazunobu Yagi +6 more
doaj +2 more sources
Correlation between beta-2-glycoprotein I gene polymorphism and anti-beta-2 glycoprotein I antibodies in patients with multibacillary leprosy [PDF]
Archives of Dermatological Research, 2010 Antiphospholipid antibodies, such as anti-beta2-glycoprotein I (beta2GPI), are present in multibacillary leprosy (MB) patients; however, MB patients do not usually present with antiphospholipid antibody syndrome (APS), which is characterized by thromboembolic phenomena (TEP).
Maria José Franco, Brochado +5 more
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Interference of lupus anticoagulant causing antiprothrombin and anti–beta-2-glycoprotein I antibodies on international normalized ratio measurements: comparative analysis of international normalized ratio methods [PDF]
Research and Practice in Thrombosis and HaemostasisBackground: Life-long vitamin K antagonist (VKA) therapy is recommended as a standard of care in antiphospholipid syndrome (APS) patients with thrombosis.
Rachel Gehlen +7 more
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Posttranslational forms of beta 2-glycoprotein I in the pathogenesis of the antiphospholipid syndrome. [PDF]
Thromb J, 2016 The antiphospholipid syndrome (APS) is an autoimmune disease characterised by a procoagulant state that predisposes to recurrent thrombosis and miscarriages. Two major discoveries have advanced our understanding of the underlying complex pathogenesis of the APS. The first was the discovery that beta-2 glycoprotein-1 (β2GPI) is the major auto antigen in
El-Assaad F +2 more
europepmc +5 more sources
PEGylated Domain I of Beta-2-Glycoprotein I Inhibits the Binding, Coagulopathic, and Thrombogenic Properties of IgG From Patients With the Antiphospholipid Syndrome [PDF]
Frontiers in Immunology, 2018 APS is an autoimmune disease in which antiphospholipid antibodies (aPL) cause vascular thrombosis and pregnancy morbidity. In patients with APS, aPL exert pathogenic actions by binding serum beta-2-glycoprotein I (β2GPI) via its N-terminal domain I (DI).
Thomas C. R. McDonnell +14 more
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