Bifidobacterium Transformation
The protocol presented in this chapter describes a generic method for electrotransformation of Bifidobacterium spp., outlining a technique that is ideal for conferring selective properties onto strains as well as allowing the user to introduce or knock out/in selected genes for phenotypic characterization purposes.
Hoedt, Emily C. +5 more
openaire +3 more sources
Bifidobacterium cuniculi, Bifidobacterium choerinum, Bifidobacterium boum, and Bifidobacterium pseudocatenulatum: Four New Species and Their Deoxyribonucleic Acid Homology Relationships [PDF]
Among the several thousand bifidobacteria of our collection, 244 strains from the feces of rabbits and suckling pigs, the rumen of cattle, the feces of breast- and bottle-fed infants, the feces of calves, and from sewage are recognized by means of deoxyribonucleic acid (DNA)-DNA hybridization (competition filter method) as belonging to four new ...
V. SCARDOVI +3 more
openaire +1 more source
Understanding how exogenous microbes stably colonize the animal gut is essential to reveal mechanisms of action and tailor effective probiotic treatments. Bifidobacterium species are naturally enriched in the gastrointestinal tract of breast-fed infants.
Britta E. Heiss +9 more
doaj +1 more source
How to choose an appropriate probiotic: a guide for clinicians [PDF]
The annual increase in probiotic usage is nothing short of phenomenal. Given the large volume of information on beneficial microorganisms, it can be challenging for clinicians to select the most suitable probiotic for a patient.
Andreeva I.V. +6 more
doaj +1 more source
The gut microbiota evolves rapidly after birth, responding dynamically to environmental factors and playing a key role in short- and long-term health. Lifestyle and rurality have been shown to contribute to differences in the gut microbiome, including ...
Muriel Derrien +12 more
doaj +1 more source
Persistence of Supplemented Bifidobacterium longum subsp. infantis EVC001 in Breastfed Infants. [PDF]
Attempts to alter intestinal dysbiosis via administration of probiotics have consistently shown that colonization with the administered microbes is transient. This study sought to determine whether provision of an initial course of Bifidobacterium longum
Andra A. Hutton +16 more
core +2 more sources
Comparison of five assays for DNA extraction from bacterial cells in human faecal samples [PDF]
Aim To determine the most effective DNA extraction method for bacteria in faecal samples. Materials and Results This study assessed five commercial methods, that is, NucliSens easyMag, QIAamp DNA Stool Mini kit, PureLink Microbiome DNA purification kit ...
Glorieux, Griet +3 more
core +1 more source
Molecular monitoring of the fecal microbiota of healthy human subjects during administration of lactulose and Saccharomyces boulardii [PDF]
A
Vanhoutte, Tom +5 more
core +2 more sources
Bifidobacterium breve reduces apoptotic epithelial cell shedding in an exopolysaccharide and MyD88-dependent manner [PDF]
Certain members of the microbiota genus Bifidobacterium are known to positively influence host well-being. Importantly, reduced bifidobacterial levels are associated with inflammatory bowel disease (IBD) patients, who also have impaired epithelial ...
K. R. Hughes +8 more
doaj +1 more source
In vitro screening of probiotic lactic acid bacteria and prebiotic glucooligosaccharides to select effective synbiotics [PDF]
Probiotics and prebiotics have been demonstrated to positively modulate the intestinal microflora and could promote host health. Although some studies have been performed on combinations of probiotics and prebiotics, constituting synbiotics, results on ...
Courtin, Céline +6 more
core +2 more sources

